Anticancer Properties of Aqueous and Nonaqueous Propolis Extracts
Abstract
|
Glioblastomas are
characterized by the highest degree of invasion and the worst survival
prediction because of the lack of efficient therapy that would not be harmful
for sensitive and vitally-important surrounding brain tissue. Propolis is a
multicomponent substance known for numerous biological effects including
antioxidant-related stimulation of immune system and tissue regeneration as
well as anticancer activity. The aim of this study was to investigate the
effects on viability of glioblastoma (C6 rat glioma cell line) and healthy
brain cells (primary cerebellar
granule cells) of aqueous (AqEP), polyethylene glycol-aqueous (Pg-AqEP)
and ethanolic (EEP) propolis extracts. After 24h
treatment, all the extracts tested decreased C6 cell viability in a
concentration-dependent manner. Statistically significant increase in the
number of dead cells was achieved at the following concentrations of phenolic
compounds: 15-18 µg/ml for AqEP, 10 µg/ml for Pg-AqEP and 5 - 7 µg/ml EEP. We
have also tested how same propolis extracts influence viability of primary cerebellar granule cell cultures
comprised of 76±3 % neurons, 16±2 % astrocytes and 8±2% microglia and found
no decrease in cell viability after 24h treatment. In conclusion,
propolis extracts exert selective cytotoxicity against C6 cells while
preserving healthy brain cells and could be recommended as adjuvant of
glioblastoma therapy as well as safe food supplements for cancer prevention. |
Keywords
Kaynakça
- .