BORİK ASİT VE PROPOLİS ENJEKTE EDİLEN YETİŞKİN SPRAGUE DAWLEY SIÇAN KARACİĞERLERİNİN WDXRF SPEKTROMETRESİ İLE ELEMENTAL ANALİZİ

Öz

Reaktif oksijen türleri (ROT) veya serbest radikallerin üretimi oksidatif stresten kaynaklanır ve oksijen veya diğer moleküller tarafından oluşturulurlar. Aşırı miktarda serbest radikaller lipitlere, proteinlere ve nükleik asitlere zarar verebilir ve karsinojeneze neden olabilir. İlaçlarda ve endüstri malzemelerinde borik asit ve propolis yoğun bir şekilde kullanılmakta ve bunların antioksidan rolleri son zamanlarda dikkat çekmektedir. Bu çalışmada, cerrahi işlemle düzensizliğe maruz kalmış ve sağlıklı yetişkin Sprague Dawley sıçan karaciğerine enjekte edilen Borik Asit (14 mg / kg) ve Propolisin (100, 200 mg / kg) karaciğerlerin iyon dengesine ve elemental kompozisyonuna etkisi araştırılmıştır. Bu amaçla Dalgaboyu Ayrımlı X-ışını Fluoresans (WDXRF) spektrometresi ile sağlıklı karaciğer dokularının elemental kompozisyonu elde edildi ve iyon konsantrasyonlarına etkisi araştırıldı. Karaciğer örneklerinde Na, Mg, Si, P, S, Cl, K, Ca, Fe, Zn, Pd ve Br majör ve minör elementlerin varlığı düşük hata oranı ile bulundu. Numunelere tek başına enjekte edilen propolis ve borik asitin Fe, Mg, Zn, Ca ve Cl elementlerinin konsantrasyonunu sağlıklı doku değerlerine yaklaştırdığı bulundu. Bu sonuç borik asit ve propolisin elementlerin üzerindeki oksidatif stresi azaltma yeteneğine atfedilebilir.

Anahtar Kelimeler

• WDXRF
• Sprague Dawley Sıçan
• Borik Asit
• Propolis
• Karaciğer

ELEMENTAL ANALYSIS OF BORIC ACID AND PROPOLIS INJECTED ADULT SPRAGUE DAWLEY RAT LIVERS BY WDXRF SPECTROMETER

Abstract

The production of reactive oxygen species (ROS) or free radicals arise from oxidative stress and they are formed by oxygen or other molecules. Excessive amounts of free radicals may damage lipids, proteins, and nucleic acids and may cause carcinogenesis. Boric acid and propolis are used extensively in pharmaceuticals and industrial materials, and their antioxidant roles have recently attracted attention. In this study, the effects of Boric Acid (14 mg / kg) and Propolis (100, 200 mg / kg) injected into exposed to vascular disorder by surgical procedure and healthy adult Sprague Dawley rat liver on the ion balance and elemental composition of the livers were investigated. For this purpose, elemental composition of healthy liver tissues was obtained by Wavelength Dispersion X-ray Fluorescence (WDXRF) spectrometry and its effect on ion concentrations was investigated. The presence of Na, Mg, Si, P, S, Cl, K, Ca, Fe, Zn, Pd and Br major and minor elements in liver samples were found with low error rates. It was found that propolis and boric acid injected into the samples alone brought the concentration of Fe, Mg, Zn, Ca and Cl elements closer to the values of healthy tissue. This result can be attributed to the ability of boric acid and propolis to reduce oxidative stress on the elements.

Keywords

• WDXRF
• Sprague Dawley Rat
• Boric Acid
• Propolis
• Liver

Kaynakça

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