Gama Radyasyonun Amitriptyline Hydrochloride Üzerinde Oluşturduğu Yapısal Bozuklukların Elektron Paramanyetik Rezonans (EPR) ve Simülasyon Tekniği ile İncelenmesi

Öz

Bu çalışmada, depresyon semptomlarının tedavisinde sıkça kullanılan ilaç etkin maddesi Amitriptyline Hydrochloride gama radyasyonuna maruz bırakılarak oluşan paramanyetik bozukluk EPR spektroskopisi ile çalışılmıştır. Örneğin, dozimetrik malzeme olarak kullanıma uygunluğu, radikal sönüm bilgileri, mikrodalga güç değerlerinde doyum bilgileri ve doz-cevap eğrisi oda sıcaklığında araştırılmıştır. İlaç örneği farklı sıcaklık aralıklarında da ölçülmüştür. Ayrıca gama radyasyonuna maruz bırakılmayan örnekte EPS sinyali gözlenmemiştir. Spektrum simülasyonu (benzetişimi) yapılarak radyasyon sonucunda oluşan radikalin spektroskopik özellikleri belirlenmiştir.

Anahtar Kelimeler

• EPR
• Dozimetri
• Gama ışınlama

Analysis of Structural Defects Caused by Gamma Radiation on Amitriptyline Hydrochloride Through Electron Paramagnetic Resonance (EPR) and Simulation Technique

Abstract

In this study, the suitable of usage as a dosimetric material gamma-irradiated Amitriptyline Hydrochloride, which is used in treatment of symptoms of depression diseases, was studied with EPR spectroscopy. Signal fading, microwave saturation behavior and dose-response curve information were investigated at room temperature. Moreover, the drug sample was also measured at different temperature ranges, EPM signal was not detected in unirradiated samples. The spectroscopic properties of radical induced by irradiation were also determined by spectrum simulation.

Keywords

• EPR
• Dosimetry
• Gamma irradiation

Kaynakça

1. Abbar, J. C., Lamani, S. D., & Nandibewoor, S. T. (2011). Ruthenium (III) Catalyzed Oxidative Degradation of Amitriptyline-A Tricyclic Antidepressant Drug by Permanganate in Aqueous Acidic Medium. Journal of Solution Chemistry, 40(3), 502–520. https://doi.org/10.1007/s10953-011-9655-9
2. Basly, J., & Bernard, M. (1997). Radio sterilization dosimetry by ESR spectroscopy: Ritodrine hydrochloride and comparison with other sympathomimetics. International Journal of Pharmaceutics, 149(1), 85–91. https://doi.org/10.1016/s0378-5173(96)04855-7
3. Basly, J., Longy, I., & Bernard, M. (1997). ESR identification of radiosterilized pharmaceuticals: latamoxef and ceftriaxone. International Journal of Pharmaceutics, 158(2), 241–245. https://doi.org/10.1016/s0378-5173(97)00257-3
4. Bhat, R., & Karim, A. A. (2009). Effect of ionizing radiation on some quality attributes of nutraceutically valued lotus seeds. International Journal of Food Sciences and Nutrition, 60(sup4), 9–20. https://doi.org/10.1080/09637480802241626
5. Cassella, J. V., & Davis, M. (1985). Fear-enhanced acoustic startle is not attenuated by acute or chronic imipramine treatment in rats. Psychopharmacology/Psychopharmacologia, 87(3), 278–282. https://doi.org/10.1007/bf00432707
6. Damian, G. (2003). EPR investigation of γ-irradiated anti-emetic drugs. Talanta, 60(5), 923–927. https://doi.org/10.1016/s0039-9140(03)00153-x
7. Finčur, N. L., Grujić-Brojčin, M., Šćepanović, M. J., Četojević-Simin, D. D., Maletić, S. P., Stojadinović, S., et al., (2021). UV-driven removal of tricyclic antidepressive drug amitriptyline using TiO2 and TiO2/WO3 coatings. Reaction Kinetics, Mechanisms and Catalysis, 132(2), 1193–1209. https://doi.org/10.1007/s11144-021-01936-7
8. Ghasemi, A., & Bagheri, A. (2020). Effects of alkyl chain length on synergetic interaction and micelle formation between a homologous series of n-alkyltrimethylammonium bromides and amphiphilic drug propranolol hydrochloride. Journal of Molecular Liquids, 298, 111948. https://doi.org/10.1016/j.molliq.2019.111948 Gibella, M., Crucq, A., & Tilquin, B. (1993). Détection RPE de l’irradiation de médicaments. Journal De Chimie Physique/Journal De Chimie Physique Et De Physico-chimie Biologique, 90, 1041–1053. https://doi.org/10.1051/jcp/1993901041

9. Jeon, M., Jun, B. M., Kim, S., Cho, J., Park, C. M., Choong, C. E., Jang, M., & Yoon, Y. (2021). Sonodegradation of amitriptyline and ibuprofen in the presence of Ti3C2Tx MXene. Journal of Hazardous Materials Letters, 2, 100028. https://doi.org/10.1016/j.hazl.2021.100028
10. Osawa, R. A., Barrocas, B. T., Monteiro, O. C., Oliveira, M. C., & Florêncio, M. H. (2020). Visible light photocatalytic degradation of amitriptyline using cobalt doped titanate nanowires: Kinetics and characterization of transformation products. Journal of Environmental Chemical Engineering, 8(1), 103585. https://doi.org/10.1016/j.jece.2019.103585
11. Polat, M., & Korkmaz, M. (2006). Effect of radiation on solid paracetamol: ESR identification and dosimetric features of γ–irradiated paracetamol. Radiation Effects and Defects in Solids, 161(1), 51–62. https://doi.org/10.1080/10420150500467471
12. Smyth, W. F., Leslie, J. C., McClean, S., Hannigan, B., McKenna, H. P., Doherty, B., et al., (2006). The characterisation of selected antidepressant drugs using electrospray ionisation with ion trap mass spectrometry and with quadrupole time‐of‐flight mass spectrometry and their determination by high‐performance liquid chromatography/electrospray ionisation tandem mass spectrometry. Rapid Communications in Mass Spectrometry/RCM. Rapid Communications in Mass Spectrometry, 20(11), 1637–1642. https://doi.org/10.1002/rcm.2485.