İndüklenmiş pluripotent kök hücreler ve hücre tedavisi

Banu - İskender; Halit Canatan

doi:10.5799/ahinjs.01.2013.04.0346

Induced pluripotent stem cells and cell therapy

Yıl 2013, , 550 - 561, 01.12.2013

Banu - İskender Halit Canatan

https://doi.org/10.5799/ahinjs.01.2013.04.0346

Öz

Human embryonic stem cells are derived from the inner cell mass of a blastocyst-stage embryo. They hold a huge promise for cell therapy with their self-renewing ability and pluripotency, which is known as the potential to differentiate into all cell types originating from three embryonic germ layers. However, their unique pluripotent feature could not be utilised for therapeutic purposes due to the ethical and legal problems during derivation. Recently, it was shown that the cells from adult tissues could be reverted into embryonic state, thereby restoring their pluripotent feature. This has strenghtened the possiblity of directed differentition of the reprogrammed somatic cells into the desired cell types in vitro and their use in regenerative medicine. Although these cells were termed as induced pluripotent cells, the mechanism of pluripotency has yet to be understood. Still, induced pluripotent stem cell technology is considered to be significant by proposing novel approaches in disease modelling, drug screening and cell therapy. Besides their self-renewing ability and their potential to differentiate into all cell types in a human body, they arouse a great interest in scientific world by being far from the ethical concerns regarding their embryonic counterparts and their unique feature of being patient-specific in prospective cell therapies. In this review, induced pluripotent stem cell technology and its role in cell-based therapies from past to present will be discussed. J Clin Exp Invest 2013; 4 (4): 550-561

Anahtar Kelimeler

Induced pluripotent stem cells, pluripotent stem cells, embryonic stem cells, regenerative medicine

Kaynakça

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Gurdon JB, Melton DA. Nuclear reprogramming in cells. Science 2008;322:1811-1815.
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Okita K, Hong H, Takahashi K, Yamanaka S. Gen- eration of mouse-induced pluripotent stem cells with plasmid vectors. Nat Protoc 2010;5:418-428.
Woltjen K, Michael IP, Mohseni P, et al. piggyBac transposition reprograms fibroblasts to induced plu- ripotent stem cells. Nature 2009;458:766-770.
Osteil P, Tapponnier Y, Markossian S, et al. Induced pluripotent stem cells derived from rabbits exhibit some characteristics of naïve pluripotency. Biol Open 2013;2:613-628.
Liu H, Zhu F, Yong J, et al. Generation of induced pluripotent stem cells from adult rhesus monkey fibro- blasts. Cell Stem Cell 2008;3:587-590.
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İndüklenmiş pluripotent kök hücreler ve hücre tedavisi

Yıl 2013, , 550 - 561, 01.12.2013

Banu - İskender Halit Canatan

https://doi.org/10.5799/ahinjs.01.2013.04.0346

Öz

İnsan embriyonik kök hücreleri embriyonun blastosist aşamasındaki iç hücre kitlesinden elde edilir. Kendi kendilerini sınırsız yenileyebilme özelliklerinin yanında pluripotent olmaları, yani üç farklı embriyonik tabakadan köken alan hücre tiplerine farklılaşabilme potansiyelleri ile hücre tedavisine yönelik büyük umut vaat etmektedirler. Ancak derivasyonlarındaki etik ve yasal problemler nedeniyle, pluripotent özelliklerinin tedavi amaçlı kullanımı henüz gerçekleştirilememektedir. Son yıllarda, erişkin dokulardan elde edilen hücrelerin yeniden programlanarak embriyonik karakter taşıyan pluripotent özellikteki hücrelere dönüşebilecekleri gösterilmiştir. Böylelikle, yeniden programlanan somatik hücrelerin, in vitro olarak istenilen hücre tipine yönlendirilmiş farklılaştırılması ve rejeneratif tıp alanında kullanılma ihtimali kuvvetlenmiştir. Pluripotent özellik kazandırılan bu hücreler indüklenmiş pluripotent kök hücreler olarak tanımlanmış olsa da, pluripotent özelliğin mekanizması henüz tam anlamıyla açıklanamamıştır. Yine de, indüklenmiş pluripotent kök hücre teknolojisi, insan hastalık modellerinin çalışılması, yeni ilaç geliştirilmesi ve hücre tedavisine yönelik yeni yaklaşımlar önermesi açısından önem arzetmektedir. Kendi kendilerini yenileyebilmeleri ve insan vücudundaki tüm hücre tiplerine farklılaşabilme potansiyellerinin yanında, embriyonik kök hücrelerin neden olduğu etik kaygılardan uzak olmaları ve olası hücre tedavisi uygulamalarında hastaya özgü tasarlanabilecek olmaları nedeniyle son yıllarda bilim dünyasında büyük ilgi uyandırmaktadırlar. Bu derlemede geçmişten günümüze indüklenmiş pluripotent kök hücre teknolojisine ve bu hücre grubunun hücre tedavisindeki yerine değinilecektir.

Anahtar Kelimeler

İndüklenmiş pluripotent kök hücreler, pluripotent kök hücreler, embriyonik kök hücreler, rejeneratif tıp

Kaynakça

Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Em- bryonic stem cell lines derived from human blasto- cysts. Science 1998;282:1145-1147.
Condic ML, Rao M. Alternative sources of pluripotent stem cells: ethical and scientific issues revisited. Stem Cells Dev 2010;19:1121-1129.
Gurdon JB, Melton DA. Nuclear reprogramming in cells. Science 2008;322:1811-1815.
Cowan CA, Atienza J, Melton DA, Eggan K. Nuclear re- programming of somatic cells after fusion with human embryonic stem cells. Science 2005;309:1369-1373.
Wilmut I, Schnieke AE, Mcwhir J, Campbell KHS. Vi- able offspring derived from fetal and adult mammalian cells. Nature 1997;385:810-813.
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663-676.
Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861-872.
Stadtfeld M, Nagaya M, Utikal J, et al. Induced plu- ripotent stem cells generated without viral integration. Science 2008;322:945-949.
Yu J, Vodyanik MA, Smuga-Otto K, et al. Induced plu- ripotent stem cell lines derived from human somatic cells. Science 2007;318:1917-1920.
Fusaki N, Ban H, Nishiyama A, et al. Efficient induc- tion of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci 2009;85:348-462.
Okita K, Hong H, Takahashi K, Yamanaka S. Gen- eration of mouse-induced pluripotent stem cells with plasmid vectors. Nat Protoc 2010;5:418-428.
Woltjen K, Michael IP, Mohseni P, et al. piggyBac transposition reprograms fibroblasts to induced plu- ripotent stem cells. Nature 2009;458:766-770.
Osteil P, Tapponnier Y, Markossian S, et al. Induced pluripotent stem cells derived from rabbits exhibit some characteristics of naïve pluripotency. Biol Open 2013;2:613-628.
Liu H, Zhu F, Yong J, et al. Generation of induced pluripotent stem cells from adult rhesus monkey fibro- blasts. Cell Stem Cell 2008;3:587-590.
Jia F, Wilson KD, Sun N, et al. A nonviral minicircle vector for deriving human iPS cells. Nat Methods 2010;7:197-199.
Huangfu D, Osafune K, Maehr R, et al. Induction of pluripotent stem cells from primary human fi- broblasts with only Oct4 and Sox2. Nat Biotechnol 2008;26:1269-1275.
Kim D, Kim CH, Moon JI, et al. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell 2009;4:472- 476.
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Toplam 124 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	Türkçe
Bölüm	Derleme
Yazarlar	Banu - İskender Bu kişi benim Halit Canatan Bu kişi benim
Yayımlanma Tarihi	1 Aralık 2013
Yayımlandığı Sayı	Yıl 2013

Kaynak Göster

APA	İskender, B. .-., & Canatan, H. (2013). İndüklenmiş pluripotent kök hücreler ve hücre tedavisi. Journal of Clinical and Experimental Investigations, 4(4), 550-561. https://doi.org/10.5799/ahinjs.01.2013.04.0346
AMA	İskender B, Canatan H. İndüklenmiş pluripotent kök hücreler ve hücre tedavisi. J Clin Exp Invest. Aralık 2013;4(4):550-561. doi:10.5799/ahinjs.01.2013.04.0346
Chicago	İskender, Banu -, ve Halit Canatan. “İndüklenmiş Pluripotent kök hücreler Ve hücre Tedavisi”. Journal of Clinical and Experimental Investigations 4, sy. 4 (Aralık 2013): 550-61. https://doi.org/10.5799/ahinjs.01.2013.04.0346.
EndNote	İskender B-, Canatan H (01 Aralık 2013) İndüklenmiş pluripotent kök hücreler ve hücre tedavisi. Journal of Clinical and Experimental Investigations 4 4 550–561.
IEEE	B. .-. İskender ve H. Canatan, “İndüklenmiş pluripotent kök hücreler ve hücre tedavisi”, J Clin Exp Invest, c. 4, sy. 4, ss. 550–561, 2013, doi: 10.5799/ahinjs.01.2013.04.0346.
ISNAD	İskender, Banu - - Canatan, Halit. “İndüklenmiş Pluripotent kök hücreler Ve hücre Tedavisi”. Journal of Clinical and Experimental Investigations 4/4 (Aralık 2013), 550-561. https://doi.org/10.5799/ahinjs.01.2013.04.0346.
JAMA	İskender B-, Canatan H. İndüklenmiş pluripotent kök hücreler ve hücre tedavisi. J Clin Exp Invest. 2013;4:550–561.
MLA	İskender, Banu - ve Halit Canatan. “İndüklenmiş Pluripotent kök hücreler Ve hücre Tedavisi”. Journal of Clinical and Experimental Investigations, c. 4, sy. 4, 2013, ss. 550-61, doi:10.5799/ahinjs.01.2013.04.0346.
Vancouver	İskender B-, Canatan H. İndüklenmiş pluripotent kök hücreler ve hücre tedavisi. J Clin Exp Invest. 2013;4(4):550-61.

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