Expression of E-cadherin in oral epithelial dysplasia and oral squamous cell carcinoma: An in vivo study

Abstract

Objectives: There is evidence that E-cadherins may also play a role in progression of dysplasia to cancer. The aim of this study was to investigate the expression of E-cadherin during the process of oral carcinogenesis by comparing their expression in normal and oral dysplastic epithelium (OED) with oral squamous cell carcinoma (OSSC). Materials and methods: The patients who were clinical suspected of having premalignant lesion and oral squamous cell carcinoma were included in the study after the histopathological diagnosis. Totally 20 cases of OED and 20 Cases of OSSC were included in the study. From each case the following data were recorded: patient\'s age and sex, tumour location, TNM classification and clinical stage (I-IV). After preliminary examination of sections stained with haematoxylin/eosin, representative parts of each piece were selected and sectioned at 5 micron for immunohistochemical study. Results: There was reduced expression of E-cadherin from mild to severe degree of dysplasia suggesting the negative correlation with E-cadherin expression. However, there was no significant correlation between E-cadherin expression, clinical TNM stage and prognosis score. Conclusion: The E-cadherin expression is reduced with increase grade of dysplasia and in oral squamous cell carcinoma. The reduced expression of E-cadherin may be a reliable indicator of increase in invasiveness in oral squamous cell carcinoma. Reduced E-cadherin expression can be considered a useful marker for progression of dysplasia to oral cancer. J Clin Exp Invest 2011; 2 (4): 347-353

Keywords

• E-cadherin, oral epithelial dysplasia, oral squamous cell carcinoma

Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma

Öz

Amaç: E-cadherin\'in displazinin kansere dönüşmesinde rol oynayabileceğine dair bulgular vardır. Bu çalışmanın amacı E-cadherin\'in ağız içi normal mukoza, yassı epitel hücreli karsinom ve displastik epitelde ekspresyonunu karşılaştırarak oral karsinogenez ile ilişkisini araştırmaktır. Gereç ve yöntem: Klinik olarak oral premalin lezyon ve yassı epitel karsinom şüphesi bulunan hastalar, histopatolojik tanıyı takiben çalışmaya alındı. Toplam olarak oral epitelyal displazili 20 hasta ve oral yassı epitel hücreli karsinomu bulunan 20 hasta çalışmaya alındı. Her hastada aşağıdaki değişkenler kaydedildi: Hastanın yaşı, cinsiyeti, tümör lokalizasyonu, TNM evresi ve klinik evre (I-IV). Hematoksilen-Eozin\'le boyanmış kesitlerin ilk muayenesini takiben, her parçadan bir kısım alınarak 5 mikronluk kesitler halinde immunohistokimyasal çalışmaya alındı. Bulgular: E-cadherin ekspresyonu ile displazi arasında negatif bir bağlantıyı telkin eder tarzda, hafiften ağıra değişen derecedeki displazide E-cadherin ekspresyonu azalmış bulundu. Ayrıca E-cadherin ekspresyonu ile klinik tümör evresi ve prognoz skoru arasında anlamlı bir bağıntı saptanmadı. Sonuç: Oral yassı hücreli karsinomda displazi derecesi arttıkça E-cadherin ekspresyonu azalmaktadır. Azalmış E-cadherin ekspresyonu ağız içi yassı epitel hücreli karsinoma\'da invazivlikte artışın güvenilir bir belirteci olabilir. Azalmış E-cadherin ekspresyonu displazini oral kansere dönüşmesini gösteren faydalı bir belirteç olarak düşünülebilir.

Anahtar Kelimeler

• E-cadherin, oral epitelyal displazi, oral yassı epitel hücreli karsinoma

Kaynakça

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