BibTex RIS Kaynak Göster

Expression of E-cadherin in oral epithelial dysplasia and oral squamous cell carcinoma: An in vivo study

Yıl 2011, , 347 - 353, 01.12.2011
https://doi.org/10.5799/ahinjs.01.2011.04.0070

Öz

Objectives: There is evidence that E-cadherins may also play a role in progression of dysplasia to cancer. The aim of this study was to investigate the expression of E-cadherin during the process of oral carcinogenesis by comparing their expression in normal and oral dysplastic epithelium (OED) with oral squamous cell carcinoma (OSSC). Materials and methods: The patients who were clinical suspected of having premalignant lesion and oral squamous cell carcinoma were included in the study after the histopathological diagnosis. Totally 20 cases of OED and 20 Cases of OSSC were included in the study. From each case the following data were recorded: patient\'s age and sex, tumour location, TNM classification and clinical stage (I-IV). After preliminary examination of sections stained with haematoxylin/eosin, representative parts of each piece were selected and sectioned at 5 micron for immunohistochemical study. Results: There was reduced expression of E-cadherin from mild to severe degree of dysplasia suggesting the negative correlation with E-cadherin expression. However, there was no significant correlation between E-cadherin expression, clinical TNM stage and prognosis score. Conclusion: The E-cadherin expression is reduced with increase grade of dysplasia and in oral squamous cell carcinoma. The reduced expression of E-cadherin may be a reliable indicator of increase in invasiveness in oral squamous cell carcinoma. Reduced E-cadherin expression can be considered a useful marker for progression of dysplasia to oral cancer. J Clin Exp Invest 2011; 2 (4): 347-353

Kaynakça

  • World Health Organization. The World Oral Health Re- port 2003. Geneva: World Health Organization; 2003. pp. 6-7
  • Massano J, Regateiro FS, Januário G, Ferreira A. Oral squamous cell carcinoma: review of prognostic and predictive factors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102(1):67-76
  • Shirayoshi Y, Okada TS, Takeichi M. The calcium-de- pendent cell-cell adhesion system regulates inner cell mass formation and cell surface polarization in early mouse development. Cell 1983; 35(5): 631-8.
  • Sutherland A. Mechanisms of implantation in the mouse: differentiation and functional importance of trophoblast giant cell behavior. Dev Biol 2003;15;258(2):241-51.
  • McNeill H, Ozawa M, Kemler R, Nelson WJ. Novel func- tion of the cell adhesion molecule uvomorulin as an in- ducer of cell surface polarity. Cell 1990;27;62(2):309- 16.
  • Boller K, Vestweber D, Kemler R. Cell-adhesion mol- ecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells. J Cell Biol 1985;100(1):327-32.
  • Takeichi M. Morphogenetic roles of classic cadherins. Curr Opin Cell Biol 1995;7(5):619-27.
  • Christofori G, Semb H. The role of the cell-adhesion molecule E-cadherin as a tumour-suppressor gene. Trends Biochem Sci 1999;24(2):73-6.
  • Warnakulasuriya S, Reibel J, Bouquot J, Dabelsteen E. Oral epithelial dysplasia classification systems: predictive value, utility, weaknesses and scope for im- provement. J Oral Pathol Med 2008; 37(3):127-133.
  • Bryne M, Nielsen K, Koppang HS, Dabelsteen E. Re- producibility of two malignancy grading systems with reportedly prognostic value for oral cancer patients. J Oral Pathol Med 1991;20(8):369-72.
  • Jankowski J, Newham P, Kandemir O, Hirano S, Takeichi M, Pignatelli M. Differential expression of E-cadherin in normal, metaplastic and dysplastic esophageal mucosa - a putative biomarker. Int J On- col 1994;4(2):441-8.
  • Santos-García A, Abad-Hernández MM, Fonseca- Sánchez E, et al. E-cadherin, laminin and collagen IV expression in the evolution from dysplasia to oral squamous cell carcinoma. Med Oral Patol Oral Cir Buccal. 2006;1;11(2):E100-5.
  • Califano J, van der Riet P, Westra W, et al. Genetic pro- gression model for head and neck cancer: implications for field cancerization. Cancer Res 1996;56(11):2488- 92.
  • Hiraguri S, Godfrey T, Nakamura H, et al. Mecha- nisms of inactivation of E-Cadherin in Breast Cancer Cell Lines. Cancer Res 1998;58(9):1972-7.
  • Kudo Y, Kitajima S, Ogawa I, et al. Invasion and me- tastasis of oral cancer cells require methylation of E-Cadherin and or degradation of membranous b- Catenin. Clin Cancer Res 2004;10(16):5455-5463.
  • Yamada K, Jordan R, Mori M, Speight PM. The re- lationship between E-cadherin expression, clinical stage and tumour differentiation in oral squamous cell carcinoma. Oral Dis 1997;3(2):82- 85.
  • Bowie GL, Caslin AW, Roland NJ, Field JK, Jones AS, Kinsella AR. Expression of cell-cell adhesion molecule E-cadherin in squamous cell carcinoma of head and neck. Clin Otolaryngol Allied Sci 1993;8(3);196-201.
  • Kim JM et al, Expression of E-cadherin and clinical correlation in patients with breast carcinoma. J Ko- rean Breast Cancer Soc 2004;7(1):148-53.
  • Williams HK, Sanders DS, Jankowski JA, Landini G, Brown AM. Expression of E-cadherin in oral epithelial dysplasia and squamous cell carcinoma J Oral Pathol Med 1998;27(7):308-17.

Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma

Yıl 2011, , 347 - 353, 01.12.2011
https://doi.org/10.5799/ahinjs.01.2011.04.0070

Öz

Amaç: E-cadherin\'in displazinin kansere dönüşmesinde rol oynayabileceğine dair bulgular vardır. Bu çalışmanın amacı E-cadherin\'in ağız içi normal mukoza, yassı epitel hücreli karsinom ve displastik epitelde ekspresyonunu karşılaştırarak oral karsinogenez ile ilişkisini araştırmaktır. Gereç ve yöntem: Klinik olarak oral premalin lezyon ve yassı epitel karsinom şüphesi bulunan hastalar, histopatolojik tanıyı takiben çalışmaya alındı. Toplam olarak oral epitelyal displazili 20 hasta ve oral yassı epitel hücreli karsinomu bulunan 20 hasta çalışmaya alındı. Her hastada aşağıdaki değişkenler kaydedildi: Hastanın yaşı, cinsiyeti, tümör lokalizasyonu, TNM evresi ve klinik evre (I-IV). Hematoksilen-Eozin\'le boyanmış kesitlerin ilk muayenesini takiben, her parçadan bir kısım alınarak 5 mikronluk kesitler halinde immunohistokimyasal çalışmaya alındı. Bulgular: E-cadherin ekspresyonu ile displazi arasında negatif bir bağlantıyı telkin eder tarzda, hafiften ağıra değişen derecedeki displazide E-cadherin ekspresyonu azalmış bulundu. Ayrıca E-cadherin ekspresyonu ile klinik tümör evresi ve prognoz skoru arasında anlamlı bir bağıntı saptanmadı. Sonuç: Oral yassı hücreli karsinomda displazi derecesi arttıkça E-cadherin ekspresyonu azalmaktadır. Azalmış E-cadherin ekspresyonu ağız içi yassı epitel hücreli karsinoma\'da invazivlikte artışın güvenilir bir belirteci olabilir. Azalmış E-cadherin ekspresyonu displazini oral kansere dönüşmesini gösteren faydalı bir belirteç olarak düşünülebilir.

Kaynakça

  • World Health Organization. The World Oral Health Re- port 2003. Geneva: World Health Organization; 2003. pp. 6-7
  • Massano J, Regateiro FS, Januário G, Ferreira A. Oral squamous cell carcinoma: review of prognostic and predictive factors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102(1):67-76
  • Shirayoshi Y, Okada TS, Takeichi M. The calcium-de- pendent cell-cell adhesion system regulates inner cell mass formation and cell surface polarization in early mouse development. Cell 1983; 35(5): 631-8.
  • Sutherland A. Mechanisms of implantation in the mouse: differentiation and functional importance of trophoblast giant cell behavior. Dev Biol 2003;15;258(2):241-51.
  • McNeill H, Ozawa M, Kemler R, Nelson WJ. Novel func- tion of the cell adhesion molecule uvomorulin as an in- ducer of cell surface polarity. Cell 1990;27;62(2):309- 16.
  • Boller K, Vestweber D, Kemler R. Cell-adhesion mol- ecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells. J Cell Biol 1985;100(1):327-32.
  • Takeichi M. Morphogenetic roles of classic cadherins. Curr Opin Cell Biol 1995;7(5):619-27.
  • Christofori G, Semb H. The role of the cell-adhesion molecule E-cadherin as a tumour-suppressor gene. Trends Biochem Sci 1999;24(2):73-6.
  • Warnakulasuriya S, Reibel J, Bouquot J, Dabelsteen E. Oral epithelial dysplasia classification systems: predictive value, utility, weaknesses and scope for im- provement. J Oral Pathol Med 2008; 37(3):127-133.
  • Bryne M, Nielsen K, Koppang HS, Dabelsteen E. Re- producibility of two malignancy grading systems with reportedly prognostic value for oral cancer patients. J Oral Pathol Med 1991;20(8):369-72.
  • Jankowski J, Newham P, Kandemir O, Hirano S, Takeichi M, Pignatelli M. Differential expression of E-cadherin in normal, metaplastic and dysplastic esophageal mucosa - a putative biomarker. Int J On- col 1994;4(2):441-8.
  • Santos-García A, Abad-Hernández MM, Fonseca- Sánchez E, et al. E-cadherin, laminin and collagen IV expression in the evolution from dysplasia to oral squamous cell carcinoma. Med Oral Patol Oral Cir Buccal. 2006;1;11(2):E100-5.
  • Califano J, van der Riet P, Westra W, et al. Genetic pro- gression model for head and neck cancer: implications for field cancerization. Cancer Res 1996;56(11):2488- 92.
  • Hiraguri S, Godfrey T, Nakamura H, et al. Mecha- nisms of inactivation of E-Cadherin in Breast Cancer Cell Lines. Cancer Res 1998;58(9):1972-7.
  • Kudo Y, Kitajima S, Ogawa I, et al. Invasion and me- tastasis of oral cancer cells require methylation of E-Cadherin and or degradation of membranous b- Catenin. Clin Cancer Res 2004;10(16):5455-5463.
  • Yamada K, Jordan R, Mori M, Speight PM. The re- lationship between E-cadherin expression, clinical stage and tumour differentiation in oral squamous cell carcinoma. Oral Dis 1997;3(2):82- 85.
  • Bowie GL, Caslin AW, Roland NJ, Field JK, Jones AS, Kinsella AR. Expression of cell-cell adhesion molecule E-cadherin in squamous cell carcinoma of head and neck. Clin Otolaryngol Allied Sci 1993;8(3);196-201.
  • Kim JM et al, Expression of E-cadherin and clinical correlation in patients with breast carcinoma. J Ko- rean Breast Cancer Soc 2004;7(1):148-53.
  • Williams HK, Sanders DS, Jankowski JA, Landini G, Brown AM. Expression of E-cadherin in oral epithelial dysplasia and squamous cell carcinoma J Oral Pathol Med 1998;27(7):308-17.
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Yazısı
Yazarlar

Monal B. Yuwanati Bu kişi benim

J. V. Tupkari Bu kişi benim

Avadhoot Avadhani Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2011
Yayımlandığı Sayı Yıl 2011

Kaynak Göster

APA Yuwanati, M. B., Tupkari, J. V., & Avadhani, A. (2011). Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma. Journal of Clinical and Experimental Investigations, 2(4), 347-353. https://doi.org/10.5799/ahinjs.01.2011.04.0070
AMA Yuwanati MB, Tupkari JV, Avadhani A. Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma. J Clin Exp Invest. Aralık 2011;2(4):347-353. doi:10.5799/ahinjs.01.2011.04.0070
Chicago Yuwanati, Monal B., J. V. Tupkari, ve Avadhoot Avadhani. “Oral Epitelyal Displazi Ve Oral Yassı hücreli Karsinomada E-Cadherin Ekspresyonu: İn Vivo Bir çalışma”. Journal of Clinical and Experimental Investigations 2, sy. 4 (Aralık 2011): 347-53. https://doi.org/10.5799/ahinjs.01.2011.04.0070.
EndNote Yuwanati MB, Tupkari JV, Avadhani A (01 Aralık 2011) Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma. Journal of Clinical and Experimental Investigations 2 4 347–353.
IEEE M. B. Yuwanati, J. V. Tupkari, ve A. Avadhani, “Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma”, J Clin Exp Invest, c. 2, sy. 4, ss. 347–353, 2011, doi: 10.5799/ahinjs.01.2011.04.0070.
ISNAD Yuwanati, Monal B. vd. “Oral Epitelyal Displazi Ve Oral Yassı hücreli Karsinomada E-Cadherin Ekspresyonu: İn Vivo Bir çalışma”. Journal of Clinical and Experimental Investigations 2/4 (Aralık 2011), 347-353. https://doi.org/10.5799/ahinjs.01.2011.04.0070.
JAMA Yuwanati MB, Tupkari JV, Avadhani A. Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma. J Clin Exp Invest. 2011;2:347–353.
MLA Yuwanati, Monal B. vd. “Oral Epitelyal Displazi Ve Oral Yassı hücreli Karsinomada E-Cadherin Ekspresyonu: İn Vivo Bir çalışma”. Journal of Clinical and Experimental Investigations, c. 2, sy. 4, 2011, ss. 347-53, doi:10.5799/ahinjs.01.2011.04.0070.
Vancouver Yuwanati MB, Tupkari JV, Avadhani A. Oral epitelyal displazi ve oral yassı hücreli karsinomada E-cadherin ekspresyonu: İn vivo bir çalışma. J Clin Exp Invest. 2011;2(4):347-53.