Insulin-like growth factor-1 receptor expression in oral squamous cell carcinoma

Abstract

Objectives: The Insulin-like growth factor-I receptor (IGF-1R) plays critical roles in cancer development, proliferation, motility and survival. IGF-1R over expression is frequently found in various tumours and is often associated with an aggressive phenotype. Hence, the aim of the present study was to examine the expression of IGF-1R in normal oral mucosa, fibroepithelial polyps, dysplastic oral mucosa and well-differentiated squamous cell carcinomas. Materials and methods: A 3-layered streptavidin peroxidase immunohistochemical method was used to detect the expression of IGF-1R in normal oral mucosa, fibroepithelial polyps, dysplastic oral mucosa and well-differentiated squamous cell carcinomas. Results: All squamous cell carcinomas (15 out of 15 patients) showed intense immunoreactivity for IGF-1R. Moderate immunoreactivity was seen in dysplastic oral lesions (12 out of 12 lesions) with positive staining in the prickle cell layer. The staining distribution in the benign lesions (14 out of 14 lesions) was weaker and similar to that seen in normal oral mucosa (10 out of 10 samples) when compared to squamous cell carcinomas and dysplastic lesions. Conclusions: Our results demonstrate increased IGF-1 receptor expression in oral squamous cell carcinomas which suggests that IGF-1 may have an important role in the development of oral cancer. J Clin Exp Invest 2011; 2 (4): 354-361

Keywords

• Growth factors, insulin-like growth factor receptor, epithelial dysplasia, Oral squamous cell carcinoma, immunohistochemistry

Insulin-like growth factor-1 receptor expression in oral squamous cell carcinoma

Öz

Amaç: İnsülin benzeri büyüme faktörü-1 reseptörü (IGF-1R)\'ün kanser gelişiminde, proliferasyon ve sağkalımda önemli rolleri vardır. IGF-1R aşırı salınımı sıklıkla çeşitli tümörlerde gözlenir ve çoğunlukla agresif fenotiple birliktedir. Bu çalışmanın amacı normal ağız içi mukozası, fibroepitelyal polipler, displastik ağız mukozası ve iyi diferensiye yassı epitel hücreli karsinom\'da IGF-1R ekspresyonunu araştırmaktır. Gereç ve yöntem: IGF-1R ekspresyonunu normal ağız içi mukozası, fibroepitelyal polipler, displastik ağız mukozası ve iyi diferensiye yassı epitel hücreli karsinom\'da araştırmak için üç basamaklı bir streptavidin peroksidaz immunohistokimyasal yöntem kullanıldı. Bulgular: Yassı epitel hücreli karsinomların tümü (15 hasta) IGF-1R için yoğun immunoreaktivite gösterdi. Orta düzeyde immun reaktivite “prickle” hücre lezyonu tabakasıyla birlikte displastik ağız içi lezyonlarda (12 hastadan 12\'sinde) görüldü. Selim lezyonlarda (14 hasta) boyama dağılımı daha zayıftı ve yassı epitel hücreli karsinom ve displastik lezyonlarla kıyaslandığında, normal ağız mukozasında (10 örnek) görülene benzer şekilde idi. Sonuç: Bulgularımız, ağız içi yassı epitel hücreli karsinom ve oral kanser gelişiminde önemli rolleri olabileceğini telkin eder tarzda, IGF-1R\'nin ekspresyonunun arttığını göstermektedir.

Anahtar Kelimeler

• Büyüme faktörleri, insülin-benzer büyüme reseptörü, epitelyal displazi, ağız içi yassı epitel hücreli karsinom, immunohistokimya

Kaynakça

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