Association of a single nucleotide polymorphism in the SUR1 gene with type 2 diabetes and obesity in Turkish patients

Yıl 2011, Cilt: 2 Sayı: 2, 161 - 167, 01.06.2011

Osman Evliyaoglu Enver Sancaktar Erkan Söğüt Mustafa Kemal Başaralı Nuriye Uzuncan Baysal Karaca

https://doi.org/10.5799/ahinjs.01.2011.02.0232

Öz

Objectives: Sulfonylurea receptor 1 (SUR1) gene codes the SUR1 protein that plays a key role in glucose-induced in-sulin secretion. In this study, we have investigated the relationship between the polymorphism in exon 31 of the SUR1 gene and type 2 diabetes, and its association with obesity in Turkish diabetic patients. Materials and methods: A total of 90 patients (45 males and 45 females, aged 43-70 years old) who were followed up in the diabetes outpatient clinic were enrolled in the study. Serum glucose, triglyceride, cholesterol and blood HbA1c levels were determined. The SNPs in exon 31 were examined by a polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) method. Results: A significant increase in the frequency of the A allele was observed in type 2 diabetic patients compared with that of the control group (41% vs. 24%, p

Anahtar Kelimeler

Type 2 diabetes, obesity, SUR1 gene polymorphism, RFLP

Türk Toplumunda Tip 2 Diyabet'in ve Obezitenin, SUR1 genindeki tek nükleotit polimorfizmi ile birlikteliği

Öz

Amaç: Sulfonylurea receptor 1 (SUR1) geni SUR1 proteinini kodlayarak glukozla indüklenen insülin sekresyonunda anahtar rolü oynar. Bu çalışmanın amacı, SUR1 geninin 31. ekzonundaki polimorfizm ile Tip 2 diyabet arasındaki ilişkiyi ve diyabetik hastalardaki obezite ile beraberliğini araştırmaktır. Gereç ve yöntem: Diyabet polikliniğinde takip edilen 90 hasta (45 kadın, 45 erkek ) çalışmaya dahil edildi. Serumda glukoz, trigliserid, kolesterol ve tam kanda HbA1c değerleri belirlendi. 31. Egzondaki SNP'ler ise polymeraz zincir reak-siyon / restriksiyon fragment uzunluk polymorphism (PCR-RFLP) yöntemiyle belirlendi. Bulgular: Kontrol grubu ile karşılaştırıldığında Tip 2 diyabetik hastalarda A allel frekansının artmış olduğu gözlendi (%41 ve %24, p

Anahtar Kelimeler

Tip 2 diyabet, obezite, SUR1 gen polimorfizmi, RFLP

Kaynakça

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