Bicarbonate may alters bacterial susceptibility to antibiotics by targeting Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus

Yıl 2019, Cilt: 9 Sayı: 3, 245 - 248, 30.09.2019

Sevgi Kesıcı , Mehmet Demırcı Ugur Kesıcı

https://doi.org/10.16899/jcm.599259

Öz

Backgrounds/aims: Acute acidemia is a common clinical
condition in critical diseases. Acidemia is associated with poor prognosis in
case of persistence. In the case of metabolic acidosis, it is beneficial to
increase the pH by administering sodium bicarbonate (NaHCO3) since cell
functions are impaired. The aim of this study was to investigate the
antibacterial efficacy of NaHCO3 used in metabolic acidosis, especially in
critically ill patients in intensive care units, and to reveal its contribution
to antimicrobial therapy for possible concomitant sepsis.

Method: S.aureus ATCC 29213, P. aeruginosa ATCC
27853 and E. coli ATCC 25922 strains were seeded into liquid Müller Hinton
medium (Oxoid, UK) and the in-vitro effect of Group C (Control - 1 mL sterile
saline) and Group B (Sodium Bicarbonate - NaHCO3) on these bacteria following
24 hours of incubation at 37 degrees^C was investigated. Following
the use of Epoch spectrophotometer (BioTek Inst. Inc. Vermont, USA) for the 0.
and 24. hours, the growth in wells was analyzed in CFU / mL and log10 CFU/mL by
comparison with the standard curve.

Results:From the start to the 24. hour, there was
a significant decrease in bacterial colony numbers of S.aureus ATCC 29213, P.
aeruginosa ATCC 27853 and E. coli ATCC 25922 strains in Group B when compared
to the control group (p <0.01).
The intra-group antibacterial efficacy comparison revealed a significant
decrease in bacterial colony numbers in Group B between 0-24 hours (p<0.01). There was a significant
increase in all bacterial colony numbers in the control group (p = 0.04).

Conclusion: In our study, NaHCO3 was found to show strong
antibacterial efficacy against P. aeruginosa, E. coli and S. aureus. Taking
these results into consideration, it should be kept in mind that the use of
NaHCO3 in the treatment of severe metabolic acidosis especially seen in septic
patients in intensive care units will also contribute to sepsis treatment
because of its antibacterial effect potential. We believe that the results of
this study, if supported by clinical studies, may contribute to the improvement
of treatment efficacy and lower treatment costs in critical patients in the
intensive care unit in the case of metabolic acidosis and sepsis.  

Anahtar Kelimeler

Antibacterial, sodium bicarbonate, Pseudomonas Aeruginosa, Staphylococcus aureus, Escherichia Coli

Kaynakça

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