The effects of CAPE on oxidative stress and histopathological values in rats treated with subacute dichlorvos

Abstract

This study was designed to investigate the protective effect of CAPE against dichlorvos induced hepatotoxicity in rats. 40 rats were used in the study, including 10 in each group. Corn oil of 5 mg/kg (solvent) was administered intragastrically to rats in the group 1, dichlorvos of 5 mg/kg was dissolved in corn oil and administered to rats in the group 2 via gavage, dichlorvos (5 mg/kg, via gavage route) and CAPE (10 μM/kg, intraperitoneally) were administered to rats of the group 3 with one-hour interval providing that CAPE treatment was started three days earlier than dichlorvos administration. The rats in the group 4 were administered with 10% solvent ethanol via intraperitoneally. All administrations continued for 15 days and blood of animals was drawn via intracardiac route after they were sacrificed by cervical dislocation under ketamine/xylazine anesthesia. Total antioxidant, total oxidant, and paraoxonase levels were measured from the obtained plasma samples. As a result of dichlorvos administration, paraoxonase activity and total antioxidant levels decreased; whereas, total oxidant levels increased. Histopathological analysis revealed that liver tissue appeared normal in control groups (1 and 4); on the other hand, there were degeneration, congestion, cellular infiltration, and necrotic areas in the group administered with dichlorvos. Even though frequency of lesions decreased, similar lesions were observed in the group with Dichlorvos+CAPE. Consequently, it was determined that while dichlorvos administration increased oxidative stress, CAPE administration had a protective potential increasing antioxidant capacity.

Keywords

• Dichlorvos; CAPE; Hepatotoxicity; Oxidative stress; Subacute administration.

References

1. Akgür SA, Öztürk P, Solak I, Moral AR, Ege B. 2003. Human serum paraoxonase (PON1) activity in acute organophorous insecticite poisoning. Forensic Sci Int. 133:136-140.
2. Alp H, Pinar N, Dokuyucu R, Sahan M, Oruc C, Kaplan I, Senol S, Ceyran AB. 2016. Protective effects of intralipid and caffeic acid phenethyl ester (CAPE) on hepatotoxicity and pancreatic injury caused by dichlorvos in rats. Biochem Genet. 54:803-815.
3. Bardin PG, Van Eeden SF, Moolman JA, Foden AP, Joubert, JR. 1994. Organophosphate and carbamate poisoning. Arc Intern Med. 154(13):1433-1441.