Ecophysiological Study on Soil Cyanobacterium in Combination of pH and Salinity Conditions at Limited Irradiance

Abstract

The aim of this research was to study ecophysiological cyanobacterium Hapalosiphon sp. FS56 collected from paddy fields of Golestan province in north of Iran. In this attempt, the purified strain was incubated in the BG011 culture medium under illumination (2 μmol quanta.m-2 .S-1) with pH 7.5 and 25 2 C temperature. In order to assess photosynthesis and morphological parameters such as growth rate, pigment and phycobilisome content, some treatments under the combination effects of pH (5,7,9), salinity condition (0, 0.25, 0.5% and 1%) at the limited irradiance (2 μmol quanta.m-2 .S-1) intensities were implemented. The Results showed that Hapalosiphon sp. Fs56 had the highest growth rate under 0.5% salinity (g/l-1), and light-saturated photosynthetic rate peaked at this salinity concentration as well. This optimal growth rate obtained at pH 9 indicated that this soil cyanobacterium is more susceptible to alkaline conditions. It should be noted that this cyanobacterium showed changes for appearance and morphology under various environmental conditions pH and salinity.

Keywords

• Cyanobacteria,Hapalosiphon,Salinity,Light,Acidity and alkalinity

Sınırlı Işınımda pH ve Tuzluluk Koşullarının Kombinasyonu ile Toprak Siyanobakteri Üzerinde Ekofizyolojik Bir Araştırma

Öz

Bu araştırmanın amacı İran'ın kuzeyinde Golestan eyaletinin çeltik tarlalarından toplanan ekofizyolojik siyanobakteri Hapalosiphon sp. FS56 nın araştırılmasıdır. Bu denemede saflaştırılmış örnekleri, BG011 kültür ortamı pH 7.5 ve 25 ° C sıcaklık koşullarında aydınlatma altında (2 umol kuant.m-2.S-1) inkübe edildi. Fotosentez ve büyüme hızı, pigment ve fikobilizom içeriği gibi morfolojik parametreleri değerlendirmek için sınırlı ışınlamada koşullarında (2 µmol quanta.m-2.S-1); pH (5,7,9) ve farklı tuzluluk durumu (% 0,025,% 0,5 ve% 1) kombinasyonları uygulanarak etkileri incelenmiştir. Sonuçlar, Hapalosiphon sp. Fs56 nın % 0.5 tuzluluk (g / l-1) altında en yüksek büyüme oranına sahip olduğunu ve doygun ışık fotosentez oranında bu tuzluluk konsantrasyonunda en yüksek noktaya ulaştığını gösterdi. PH 9'da elde edilen bu optimal büyüme oranı, bu toprak siyanobakterisinin alkalin koşullarına daha duyarlı olduğunu göstermiştir. Bu siyanobakterinin, çeşitli çevresel pH ve tuzluluk koşulları altında görünümünde ve morfolojisinde değişiklikler gösterdiği fark edilmelidir.

Anahtar Kelimeler

• Siyanobakteri,hapalasifon,tuzluluk,ışık,asidete ve baziklik,morfoloji,İran

Kaynakça

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