PRELIMINARY STUDY OF DNA ЕXTRACTION FROM BULGARIAN HONEYS AND ITS AMPLIFICATION BY PCR FOR BOTANICAL IDENTIFICATION

Abstract

Some current commonly used methods for identification of botanical origin of honey involve melissopalynological analysis or other analytical methods. However, these methods can be less sensitive and time-consuming. DNA-based methods have become popular due to their quickness and reliability. In this respect, the purpose on the present study is to compare two DNA extraction kits – DNeasy Plant Mini Kit (Qiagen) and GeneJET Genomic DNA Purification Mini Kit (Thermo Scientific) and CTAB method combined with different pre-treatment procedures and applied to 3 multifloral and 2 monofloral honey samples. The DNA extraction and amplification was carried out for all honey samples with similar results in each replication. The extracted DNA was amplified by PCR using plant specific primers rbcL gene and trnH-psbA spacer. The result obtained confirming that the DNA extracted has good quality for analysis of PCR products and that it can be used for botanical identification of honey. 

Keywords

• rbcL,trnH-psbA spacer,DNA еxtraction,Honey,Botanical identification

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