3D Baskılı PLA-İskelelerin Üzerinde Farklılaştırılmış Mezenkimal Kök Hücrelerin In Vitro ve In Vivo Uyumları

Öz

Üç boyutlu baskı, doku mühendisliği uygulamaları için biyolojik olarak uyumlu ve biyolojik olarak parçalanabilen malzemelerin üretim aracı olarak hızlı bir giriş yaptı. Üç boyutlu baskı, özel biyobozunur implantlar oluşturmayı mümkün kıldı ve polilaktik asit (PLA) en umut verici polimerlerden biridir. Bu çalışmada, 3D baskı PLA polimerleri üzerinde kemik iliği stromal mezenkimal kök hücrelerinden (BMSC) hem kemik hem de kıkırdağa farklılaştırılan hücreler oluşturmayı amaçladık. PLA iskeleleri tasarlanmış, Solidworks Yazılımı kullanılarak 3D olarak basılmış ve Yakın Doğu Üniversitesi'nin özel tesislerinde sterilize edilmiştir. Mezenkimal kök hücreler sıçan kemik iliğinden toplandı ve daha sonra osteoblast veya kondroblastta farklılaştırıldı. Hücrelerin karakterizasyonu Alizarin kırmızısı, Alcian mavisi boyama, osteonektin ve kollajen II kullanılarak indirekt immünositokimya ile analiz edildi. Farklılaştırılmış hücreler, 3D yapı iskelesine ekilerek 2 hafta boyunca kültüre edildi. İn vivo test için, farklılaştırılmış BMSC'leri olan veya olmayan 3D yapı iskelesi, subkutan alanın bağ dokusuna implante edildi. Dört aylık implantasyondan sonra sıçanlar sakrifiye edildi ve tüm numuneler histokimyasal ve immünohistokimyasal olarak incelendi. BMSC'den osteojenik ve kondrojenik farklılaşma 2 haftalık kültür koşulundan sonra gözlendi. Hücreler hem histokimyasal hem de immünohistokimyasal olarak pozitif boyandılar. Hücreler 3D PLA iskelesine aktarıldıktan sonra farklılaşmaları devam etmiş ve hem in vitro hem de in vivo koşullarda histokimyasal ve immünohistokimyasal analizlerden sonra hem kemik hem de kıkırdak oluşumu gözlemlenmiştir. 3D baskılı PLA iskeleleri hem kemik hem de kıkırdak oluşumunu desteklediği ve bu nedenle bu hücrelerin in vivo çalışmalar için rahatlıkla kullanılabileceği gözlenmiştir.

Anahtar Kelimeler

• Kemik
• Kıkırdak
• 3D baskı
• Mezenkimal Kök Hücre

Kaynakça

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