Fibrinojen Tayin Yöntemlerinin Karşılaştırılması: Tarihsel Pencereden Bakış (Modifiye Stirland, Clauss Kronometrik ve Sodyum Sülfit ile Fraksiyonizasyon Yöntemlerinin Karşılaştırılması)

Öz

Günümüzde otoanalizörlerde kullanılan Clauss kronometrik yönteminin manuel versiyonu, daha eski bir metotla, Modifiye Stirland yöntemi ile karşılaştırılmıştır. Fibrinojenin sodyum sülfit ile presipitasyonu ve ardından fraksiyone edilen fibrinojenin Biüret yöntemi ile protein olarak ölçülmesi referans yöntem olarak kullanılmıştır. Çalışma aynı zamanda otoanalizörler ile rutin laboratuvar çalışmalarına dahil olan uzman ve öğrencilerin fibrinojen testlerinin tarihsel evrimi hakkında fikir oluşturmasına yardımcı olacaktır. Biüret yöntemine dayalı referans ve Modifiye Stirland yöntemi karşılaştırıldığında, arasında anlamlı fark bulunmuştur (t= -6,075; p<0.05). Referans ve Clauss kronometrik yöntemi arasındaki fark istatistiksel yönden anlamlı bulunmamıştır (t=-1.833; p= 0.076). Modifiye Stirland ve referans yöntemi arasındaki korelasyon yüksek düzeyde anlamlı (r= 0.898; p<0.001); referans ve Clauss kronometrik yöntemi arasındaki korelasyon da yüksek düzeyde anlamlı bulunmuştur. (r= 0.845; p<0.001). Her yöntemin deney içi tekrarlanabilirliği için averaj varyasyonlar hesaplanmıştır. Bu değerler Biüret yöntemine dayalı referans, modifiye Stirland ve Clauss kronometrik yöntemi için sırasıyla % 5.77; %6.78 ve %2.56 dür. Fibrinojen düzeylerinin ölçümünde manuel versiyonu kullanılmış olan Clauss kronometrik yöntemi, Modifiye Stirland yöntemine oranla daha başarılı bulunmuştur. Manuel olarak uygulanan Clauss kronometrik yöntemi günümüzde otoanalizörlerde çalışılan bir metod şeklinde günlük biyokimya laboratuvar tıbbında yerini korumaktadır.

Anahtar Kelimeler

• Fibrinojen
• yöntem karşılaştırma
• klinik kimya
• analiz

Comparison of Fibrinogen Determination Methods Looking from the Historical Window (Comparison Of Modified Stirland, Clauss Chronometric and Sodium Sulfite Fractionation Methods)

Öz

The manual version of the Clauss chronometric method which is used in autoanalysers today, was compared with the Modified Stirland method, which was older than Clauss chronometric method. Fractionation of fibrinogen which was precipitated with sodium sulfite followed determination as a protein by Biuret method was used as the reference method. The study will also help experts and students involved in routine laboratory work with autoanalysers to provide insight into the historical evolution of fibrinogen testing.
Significant difference was found between the reference method which was based on determination of fibrinogen by Biuret reagent and the Modified Stirland method (t= -6,075; p<0.05). The difference between the Reference and Clauss chronometric method was not statistically significant (t=-1.833; p=0.076). The correlation between the modified Stirland and the reference method is highly significant (r= 0.898; p<0.001); Correlation between reference and Clauss chronometric method was also found highly significant. (r = 0.845; p <0.001). Average variations were calculated for each method. These values are 5.77%. 6.78% and 2.56%, respectively, for reference, Modified Stirland and Clauss chronometric method; The Clauss chronometric method, which was used in the manual version for measuring fibrinogen levels, was found to be more successful than the Modified Stirland method. Clauss chronometric method, which was applied manually, still maintains its place in daily biochemistry laboratory medicine as in fully automated auto analyzers.

Anahtar Kelimeler

• Fibrinogen
• method comparison
• chemistry
• clinical chemistry

Kaynakça

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