Çevresel Kirletici Etofenproks'un Zebra Balıklarında (Danio rerio) Subletal Genotoksik Etkileri

Yıl 2020, Cilt: 4 Sayı: 1, 14 - 18, 15.03.2020

Neslihan Ağırbaşlı Aysel Çağlan Günal Gülsüm Koçak Aylin Sepici Dinçel

https://doi.org/10.35333/JOHSE.2020.190

Öz

Bu çalışmada ekotoksikolojik araştırmalarda model
organizmalardan olan zebra balığı (Danio rerio), etofenproksun
sucul ekosistemler üzerindeki öldürücü etkilerini belirlemek
için kullanılmıştır. Ester olmayan sentetik piretroid
etofenproks (2-(4-etoksifenil)-2-metilpropil 3-fenoksibenzileter
phenoxybenzylether), haşere kontrol programları ile direkt su
aracılığı ile ya da dolaylı olarak yağmur suları ve yüzey suları
ile vücuda alınabilir. Deney grupları 96. saat LC50 değeri 1/10
(8.1 μg/L) ve 1/100 (0.81 μg/L) dozunda etofenproksa 48 ve
96 saat boyunca maruz bırakılmıştır. Oksidatif DNA hasarını
değerlendirmek için tüm vücut zebra balıkları homojenize edilerek
DNA izolasyonu yapıldı. Daha sonra DNA örnekleri hidrolize
edilerek, oksidatif hasar 8-hidroksi-2’deoksiguanozin (8OHdG,
ng/g doku) olarak enzim immun yöntem ile ölçülmüştür. Kontrol
grubu ile karşılaştırıldığında her iki grupta, düşük ve yüksek her
iki dozda 8OHdG düzeyleri yüksek gözlendi. DNA hasar düzeyi
96. saat yüksek ve düşük doz etofenproksa maruz bırakılan grup
ile 48. saat etofenproksa maruz kalan grup ile karşılaştırıldığında
her iki dozda istatistiksel olarak anlamlı yüksek bulundu. Sonuç
olarak subletal konsantrasyonlarda etofenproksa maruziyetin
zebra balıklarında akut genotoksik etki gösterdiği ve doku hasarına
yol açtığı, maruziyet süresinin devamı ile tamir mekanizmalarının
etkin olabileceği düşünülmektedir.

Anahtar Kelimeler

Etofenproks, Zebra balığı, DNA hasarı, Çevresel kirletici

Destekleyen Kurum

Gazi Üniversitesi Bilimsel Proje Birimi

Proje Numarası

01/2015-38

The Sublethal Genotoxic Effects of Environmental Pollutants of Etofenprox on Zebrafish (Danio rerio)

Öz

ecotoxicological research, was used to determine the sublethal
effects of etofenprox on aquatic ecosystems. Non-ester synthetic
pyrethroid etofenprox (2-(4-ethoxyphenyl)-2-methylpropyl
3-phenoxybenzylether) can be taken into the body either by direct
water or indirectly with rainwater and surface waters of pest control
programs. Experimental groups were exposed to etofenprox for 48
and 96 hours at the 96th hour LC50 1/10 (8.1 μg/L) and 1/100 (0.81
μg/L) dose. In order to evaluate genomic oxidative DNA damage,
whole body zebra fish were homogenized and DNA isolation was
performed. DNA samples are then hydrolyzed and the oxidative
damage was measured by commercial kit as EIA. Compared to
the control group, low and high doses of 8OHdG in both groups
were high. DNA damage level was found to be statistically
significantly higher in both doses compared to the 96th hour group
exposed to high and low dose etofenprox and the 48th hour group
exposed to etofenprox. As a result, it is suggested that the sublethal
concentrations of etofenprox has acute genotoxic effect in zebra
fish and causes tissue damage and related with the duration of
exposure repair mechanisms may be effective.

Anahtar Kelimeler

Etofenprox, Zebra fish, DNA damage, Environmental pollutants

Proje Numarası

01/2015-38

Kaynakça

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