Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection

Yıl 2018, En çok Erişilen Makaleler, 68 - 75, 05.06.2018

Fariha Akhter Chowdhury, Et Al.

https://doi.org/10.5455/jicm.27.20171014

Öz

Background: Urinary
tract infection (UTI) is frequently encountered by female population where the
episode of occurrence increases with advancing age. Escherichia coli, a common UTI causing organism, retains
glutathione defense mechanisms that may allow the organism to withstand host
oxidative immune response as well as the harsh physiological environment of
urinary tract. The aim of this study was to analyze glutathione reductase (GR)
activity of UTI causing E. coli under
stressful condition. Material and
Methods: E. coli isolates from
urine samples of UTI female patients of different ages were sampled. Samples of
isolated E. coli were grown in
conditions with and without oxidative stress where the stressful condition was
induced by hydrogen peroxide (H₂O₂). In both conditions
measurement of reduced glutathione level of the isolates was performed
calorimetrically using microplate reader assay. Results: Samples from elderly patients showed that GSH level
significantly altered, the value of GSH (mM/mg) are 5.01±0.48 and 5.20±0.64 in
normal and under stressful condition respectively, which showed increase of GSH
level by 3.83% (p = 0.024) under oxidative stress condition. On the other hand,
in case of adult patients, GSH (mM/mg) level found to be decreased by 5.11% (p
= 0.011) with the values of 5.08±0.1 and 4.82±0.18 respectively for normal and
stressed condition. Conclusion: Our
data suggesting an association of GR activity with patient’s age, may signify
that E. coli isolates respond
differently to oxidative stressful environment in different age-related
physiological changes and could help to uncover ways to gain better insight
into E. coli pathogenesis of UTI
predominance in aged population.

Anahtar Kelimeler

Aged, Escherichia coli, glutathione reductase, oxidative stress, reduced glutathione (GSH), Urinary tract infections (UTI)

Kaynakça

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