Kadmiyum ile Oksidatif Strese Maruz Kalan Sıçanların Beyin Dokusunda Astaksantinin Etkisi;Biyokimyasal, Histopatolojik Değerlendirme

Öz

Bu çalışmada, ratlarda kadmiyum (Cd), ile meydana gelen beyin hasarını önlemede astaksantinin (AST),

koruyucu etkisinin değerlendirilmesi amaçlandı. 32 adet Winstar albino cinsi rat kontol, Kadmiyum (Cd), Astaksantin

(AST), Kadmiyum (Cd)+Astaksantin (AST), olmak üzere dört gruba ayrıldı. 30. Günün sonunda rat beyin dokuları

alındı. Beyin doku homojenatında malondialdehit (MDA), Glutatyon (GSH) seviyeleri ve süperoksitdismutaz (SOD)

enzim aktivitesi ölçülerek, histopatolojik inceleme yapıldı. Beyin dokusu MDA, düzeyleri değerlendirildiğinde

kontrol grubu ile kıyaslandığında Cd uygulaması yapılan grupta (p<0,01) ve yine Cd+AST uygulanan grupta artış

(p<0,05) olduğu belirlendi. Cd uygulaması yapılan gruba oranla Cd+AST grubunda görülen azalmanın istatistiksel

olarak önemli olduğu belirlenmişdir (p<0,01). GSH düzeyleri değerlendirildiğinde, Cd ve Cd+AST grupları,

kontrol grubu ile kıyaslandığında gruplar arasında istatistiksel olarak azalışın olduğu belirlendi (p<0,05). SOD

enzim aktivitesi değerlendirildiğinde ise Cd ve Cd+AST gruplarında, kontrol grubuna kıyasla görülen azalışın

(p<0,01) istatistiksel olarak önemli olduğu, Cd grubuna oranla, Cd+AST uygulaması yapılan grupta SOD düzeyinde

görülen artışın önemli olduğu belirlendi (p<0,05). Histopatalojik bulgular değerlendirildiğinde, Serebral korteks

ve hipokampusta histopatolojik bulgular gruplar arasında farklılık gösterdi. Kontrol ve AST uygulanan gruplarda

beyinde normal histolojik yapı gözlenirken, Cd uygulanan grupta ciddi lezyonlar görüldü. Cd+AST grubunda hafif

dejeneratif lezyonlar gözlendi. Sonuç olarak, Cd uygulamasına bağlı olarak artan MDA düzeyi, AST uygulması ile

azaldı. Cd uygulaması ile azalan GSH düzeyi ve SOD enzim aktivitesi AST uygulaması ile arttı. Aynı zamanda AST

uygulmasının histopatolojik lezyonları azalttığı gözlemlendi. Bundan dolayı, AST’nin Cd kaynaklı beyin oksidatif

hasara karşı koruyucu olarak kullanılabileceği düşünülmektedir.

Anahtar Kelimeler

• Astaksantin,Kadmiyum,Oksidatif stres

Effect Of Astaxanthin On Rat Brains Against Oxidative Stress Induced By Cadmium:Biochemical, Histopathological Evaluation

Öz

Aim of this study is to evaluate protective impact of Astaxanthin (AST) on rats with experimental

brain injury induced with Cadmium (Cd). 32 male Wistar albino rats were divided into four groups as Control,

Cadmium, Astaxanthin (AST), Cadmium (Cd)+Astaxanthin (AST). Rat brain tissues were obtained at the end

of 30th day. Malondialdehyde (MDA), glutathione (GSH) levels and superoxide dismutase (SOD) enzyme

activities were measured in brain homogenates and histopathological examination was performed. MDA levels

were improvement in cadmium administered group (p<0.01) as well as Cd+AST administered group (p<0.05)

compared to control group. In addition a substantial reduction Cd+AST group was observed compared to cadmium

administered group (p<0.01). GSH level shows a decrease in Cd and Cd+AST groups compared to control (p<0.05).

SOD enzyme activity was found significantly lower in Cd and Cd+AST groups compared to control (p<0.01). In

addition, increase of SOD in Cd+AST group compared to cadmium group was also found significant (p<0.05).

Histopathological findings in the cerebral cortex and hippocampus were different between groups. In the control

and AST administered groups, normal histological structure was observed in the brain, while severe lesions were

seen in the Cd administered group and in the Cd+AST group only mild degenerative lesions were observed.

As a result, elevated MDA level due to Cd administration was attenuated with AST administration. Decreased GSH

level and SOD enzyme activity due to Cd administration was increased with AST administration. In addition, AST

administration decreased histopathological lesions. Consequently, it is thought that AST may be used for protection

against brain oxidative damage due to Cd.

Anahtar Kelimeler

• Astaxanthin,cadmium,oxidative stress

Kaynakça

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