Change of Insulin-Like Growth Factor-1 in Lactation Period in White Goats

Öz

In this study, change of IGF-I in lactation period was investigated on 11 heads female white goat (6 heads 2 aged and 5 heads 3 aged) raised at the farm of Department of Animal Science, Faculty of Agriculture, Ankara University, Turkey. To determine IGF-I concentrations, the blood samples were regularly taken from jugular vein of goats in every two weeks during lactation period. The blood samples were centrifuged at 4000xg for 5 min. and serum was stored at -20 °C until analysis time. IGF-I analysis in the blood serum were performed by enzyme immunoassay (EIA) method. Climatic values of the experimental period were obtained from the local meteorological service and the temperature-humidity index (THI) was calculated. In the study, the observed IGF-I concentrations with respect to periods and age groups was analyzed by the repeated measures of ANOVA analysis. Significant differences (P<0.01) were found among the periods for IGF-I concentrations. In conclusion IGF-I concentrations were significantly influenced by photoperiod changes and IGF-I releases were depend on increased length of day during lactation period in White goats.

Anahtar Kelimeler

• IGF-I,White goat,lactation,photoperiod,temperature,THI

Akkeçilerde İnsülin Benzeri Büyüme Faktörü-I’in (IGF-I) Laktasyon Dönemindeki Değişimi

Öz

Bu araştırmada, Ankara Üniversitesi Ziraat Fakültesi Zootekni Bölümü Hayvancılık İşletmesinde yetiştirilen 6 baş 2 yaşlı ve 5 baş 3 yaşlı olmak üzere toplam 11 baş dişi Akkeçide laktasyon döneminde IGF-I’in değişimi incelenmiştir. Çalışmada, IGF-I konsantrasyonlarını belirlemek için laktasyon dönemi boyunca keçilerin boyun toplardamarından her iki haftada bir düzenli olarak kan örnekleri alınmıştır. Kan örnekleri 4000 devir/dak’da 5 dakika süreyle santrifüj edilmiş ve serumlar -20 °C’de laboratuvar analizleri gerçekleştirilene kadar muhafaza edilmiştir. Kan serumunda IGF-I analizleri enzim immunoassay (EIA) yöntemiyle gerçekleştirilmiştir. Araştırmanın gerçekleştirildiği döneme ait meteorolojik veriler Meteoroloji Genel Müdürlüğü’nden temin edilmiş ve bu değerlerden sıcaklık-nem indeksi (SNİ) hesaplanmıştır. Araştırmada, IGF-I konsantrasyonları bakımından dönemler ve yaş grupları arasındaki farklılıklar, tekrarlanan ölçümlü varyans analizi tekniği ile değerlendirilmiştir. İstatistik analiz sonucunda, sadece incelenen dönemler arasındaki farklılıklar istatistik olarak önemli (P<0.01) bulunmuştur. Sonuç olarak, Akkeçilerde laktasyon döneminde, IGF-I konsantrasyonlarının fotoperiyot değişimlerinden önemli düzeyde etkilendiği ve gün uzunluğundaki artışa bağlı olarak IGF-I salınımının arttığı belirlenmiştir.

Anahtar Kelimeler

• Akkeçi,IGF-I,laktasyon,fotoperiyot,sıcaklık,SNİ

Kaynakça

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