Akkeçilerde İnsülin Benzeri Büyüme Faktörü-I’in (IGF-I) Laktasyon Dönemindeki Değişimi

Year 2019, , 1193 - 1200, 01.06.2019

Naciye Melis Terzi , Erkan Pehlivan , Gürsel Dellal

https://doi.org/10.21597/jist.469393

Abstract

Bu araştırmada, Ankara Üniversitesi Ziraat Fakültesi Zootekni Bölümü Hayvancılık İşletmesinde yetiştirilen 6 baş 2 yaşlı ve 5 baş 3 yaşlı olmak üzere toplam 11 baş dişi Akkeçide laktasyon döneminde IGF-I’in değişimi incelenmiştir. Çalışmada, IGF-I konsantrasyonlarını belirlemek için laktasyon dönemi boyunca keçilerin boyun toplardamarından her iki haftada bir düzenli olarak kan örnekleri alınmıştır. Kan örnekleri 4000 devir/dak’da 5 dakika süreyle santrifüj edilmiş ve serumlar -20 °C’de laboratuvar analizleri gerçekleştirilene kadar muhafaza edilmiştir. Kan serumunda IGF-I analizleri enzim immunoassay (EIA) yöntemiyle gerçekleştirilmiştir. Araştırmanın gerçekleştirildiği döneme ait meteorolojik veriler Meteoroloji Genel Müdürlüğü’nden temin edilmiş ve bu değerlerden sıcaklık-nem indeksi (SNİ) hesaplanmıştır. Araştırmada, IGF-I konsantrasyonları bakımından dönemler ve yaş grupları arasındaki farklılıklar, tekrarlanan ölçümlü varyans analizi tekniği ile değerlendirilmiştir. İstatistik analiz sonucunda, sadece incelenen dönemler arasındaki farklılıklar istatistik olarak önemli (P<0.01) bulunmuştur. Sonuç olarak, Akkeçilerde laktasyon döneminde, IGF-I konsantrasyonlarının fotoperiyot değişimlerinden önemli düzeyde etkilendiği ve gün uzunluğundaki artışa bağlı olarak IGF-I salınımının arttığı belirlenmiştir.

Keywords

Akkeçi, IGF-I, laktasyon, fotoperiyot, sıcaklık, SNİ

Supporting Institution

Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

16L0447003

Thanks

Bu çalışma, Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından “16L0447003” kodlu proje ile desteklenmiştir.

Change of Insulin-Like Growth Factor-1 in Lactation Period in White Goats

Abstract

In this study, change of IGF-I in lactation period was investigated on 11 heads female white goat (6 heads 2 aged and 5 heads 3 aged) raised at the farm of Department of Animal Science, Faculty of Agriculture, Ankara University, Turkey. To determine IGF-I concentrations, the blood samples were regularly taken from jugular vein of goats in every two weeks during lactation period. The blood samples were centrifuged at 4000xg for 5 min. and serum was stored at -20 °C until analysis time. IGF-I analysis in the blood serum were performed by enzyme immunoassay (EIA) method. Climatic values of the experimental period were obtained from the local meteorological service and the temperature-humidity index (THI) was calculated. In the study, the observed IGF-I concentrations with respect to periods and age groups was analyzed by the repeated measures of ANOVA analysis. Significant differences (P<0.01) were found among the periods for IGF-I concentrations. In conclusion IGF-I concentrations were significantly influenced by photoperiod changes and IGF-I releases were depend on increased length of day during lactation period in White goats.

Keywords

IGF-I, White goat, lactation, photoperiod, temperature, THI

Project Number

16L0447003

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