Evaluation of resistance to fusidic acid in Staphylococci isolates

Abstract

Objectives: Staphylococci were most prominent factors that responsible for skin, soft tissue and foreign body infections. Gaining resistance to methicillin and various antibiotics in these microorganisms over the years increased morbidity and mortality, especially in hospitalized patients. In such situations number of preferred antibiotics was limited. The aim of this study was to investigate in-vitro susceptibility of fusidic acid to clinic isolates of Staphylococci. Materials and methods: The seventy-seven coagulase negative staphylococci (CNS) and 37 Staphylococcus aureus strains isolated from various clinical specimens were included in this study. Staphylococci were identified with gram stain, catalase and coagulase tests. According to "Clinical and Laboratory Standards Institute (CLSI)" criteria, antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method. Results: The seventy-four percent of the isolated CNS were defined as methicillin sensitive-CNS (MS-CNS), 26% of the isolated CNS were defined as methicillin resistant (MR-CNS). While 40% of MR-CNS was resistant to fusidic acid, fusidic acid resistance was found to be 24% in MS-CNS. In methicilline-sensitive S.aureus strains fusidic acid resistance rate were detected as 13%; and in methicillin-resistant S.aureus strains the rate were 14%. Conclusion: Susceptibility of fusidic acid in Staphylococcus aureus strains were found higher than coagulase-negative staphylococci. Fusidic acid remains as an alternative in the treatment of infections due to staphylococci.

Keywords

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Evaluation of resistance to fusidic acid in Staphylococci isolates

Öz

Anahtar Kelimeler

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Kaynakça

1. Akcay SS, Oguzoglu N, Inan AS, Kucukercan M, Cobanoglu F. Fucidic acid and mupirocin sensitivity of methicillin-resistant Staphylococcus aureus isolated from skin and soft tissue in- fections. Klimik Derg 2005;18:117-120.
2. Eksi F, Gayyurhan ED, Bayram A. Antimicrobial susceptibility of Staphylococcus aureus strains isolated in Gaziantep Uni- versity Hospital. Ankem Derg 2008;22:203-208.
3. Yaman G, Cıkman A, Berktas M, Parlak M, Guducuoglu H, Karahocagil MK. The MLSB, fusidic acid and various antibi- otic resistance rates of nosocomial Staphylococcus aureus isolates. Ankem Derg 2010;24:130-135.
4. Clinical and Laboratory Standards Institute (CLSI) (Çeviri ed. D Gür): Performance standards for antimicrobial susceptibil- ity testing; twenty-first informational supplement. Replaces M100-S16. Pennsylvania, USA 2009.
5. Comite de L’Antibiogramme de la Socit Française de Microbi- ologie Communique: Path Biol 1996;44:1-5.
6. Sengoz G, Yıldırım F, Kart-Yasar K, Sengoz A, Nazlıcan O. Resistance of Staphylococcus strains against fusidic acid and other antibiotics. Ankem Derg 2004;18:105-108.
7. Baysal B, Tuncer I, Erayman B, Arslan U. Susceptibility of clinical isolates of Staphylococcus aureus to fusidic acid and various antibiotics. Infeksiyon Derg 2003;17:27-30.
8. Altun B, Kocagoz S, Hascelik G, Uzun O, Akova M, Unal S. Susceptibilities to fusidic acid and frequently used antibiotics of Staphylococcus strains isolated in various hospitals. Turk Mikrobiyol Cem Derg 2003;33:8-11.

9. Ozcan N, Durmaz CB, Oktar M. Investigation of resistance rates to various antibiotics of Staphylococci strains isolated from the wound samples. XI. Türk Klinik Mikrobiyoloji ve İnfeksiyon Hastalıkları Kongresi, 30 March-3 April, İstanbul- Turkiye, 2003. Klimik Derg 2003;16(suppl 1):350.
10. Ulug M, Ayaz C, Celen MK. Fusidic acid’s place in the treat- ment of chronic staphylococcal osteomyelitis. Anatol J Clin Invest 2009;3:222-226.
11. Moorhouse E, Fenelon L, Hone R. Staphylococcus aureus sensitivity to various antibiotics. Ir J MedSci 1996;165:40.
12. Coombs RR, Menday AP. Fusidic acid in orthopaedic infec- tions due to coagulase-negative staphylococci. Curr Med Res Opin 1985;9:587-590.
13. McLaws F, Chopra I, O’Neill AJ. High prevalence of resis- tance to fusidic acid in clinical isolates of Staphylococcus epidermidis. Antimicrob Chemother 2008;61:1040-1043.
14. Katopodis GD, Grivea IN, Tsantsaridou AJ, et al. Fusidic acid and clindamycin resistance in community-associated, meth- icillin-resistant Staphylococcus aureus infections in children of Central Greece. BMC Infectious Diseases 2010;10:351- 353.
15. Erdemoglu A, Ozsoy MF, Emekdas G, Oncul O, Pahsa A. The resistance of Staphylococci isolated from urine to fu- sidic acid and other antimicrobials. Türk Mikrobiol Cem Derg 2000;30:6-12.
16. Yazgı H, Ertek M, Aktas O. Investigation of fusidic acid sus- ceptibility of Staphylococcus strains isolated from various clinical specimens. Türk Mikrobiyol Cem Derg 2003;33:12- 15.
17. Celen MK, Ayaz C, Ozmen E, Geyik MF, Hosoglu S. Resis- tance to fucidic acid in clinical Staphylococcus aureus iso- lates. Klimik Derg 2005;18;114-116.
18. Dogruman-Al F, Akca G, Sipahi B, Sultan N. Evaluation of antimicrobial resistance in Staphylococcal species isolated from blood cultures. Ankem Derg 2005;19:14-16.
19. Nergiz S, Ozekinci T, Gulhan B, Mese S, Atmaca S. Resis- tance to fusidic acid in methicillin-resistant coagulase nega- tive Staphylococci isolated from various clinical specimens. Ankem Derg 2007;21:228-231.
20. Mert Dinc B, Karabiber N, Aykut Arca E. Macrolide-Lincos- amide-Streptogramin B (MLSB) resistance and fusidic acid susceptibility of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from clinical samples. Türk Hijyen ve Deneysel Biyoloji Derg 2009;66:89-94.