Transcriptional expression of PmrB and arnA within polymyxin-resistant nosocomial isolates of Pseudomonas aeruginosa from India

Yıl 2018, , 61 - 68, 20.06.2018

Sayani Roy Deepjyoti Paul Rajkumari Elizabeth Debadatta Dhar Atanu Chakarvarty Amitabha Bhattacharjee

https://doi.org/10.5799/jmid.434595

Öz

ABSTRACT

Objective: The polymyxin group of antibiotics is considered to be
one of the most effective antimicrobial agents against many serious pathogenic
bacteria, but the excessive use of these antibiotics has led to the development
of drug resistance among bacteria. This study was designed to characterize polymyxin-resistant
P. aeruginosa and to explore the role
of PmrB and arnA in resistant phenotype.

Methods:
mRNA and cDNA of five selected polymyxin-resistant strains representing
different MIC range; isolated under normal condition of  strain growth, after treating sample/media
with FeCl3 and MgCl2 alone, or after treating with FeCl3 and polymyxin
antibiotic. The transcriptional expression was observed for PmrB and arnA by quantitative real time RT-PCR in reference to P. aeruginosa PAO1. The presence of
plasmid mediated colistin resistance determinants mcr-1 was screened by PCR. Susceptibility of the strains was
determined by disc-diffusion method and DNA fingerprinting was carried out by
performing REP-PCR.

Results: A
down regulated expression of PmrB and
arnA was observed even after the
unique induction with FeCl3 and MgCl2. All the isolates were found to be
resistant against cefepime and different clonal patterns of resistance were
found among the isolates.

Conclusion: This study has drawn a new insight into polymyxin
resistance which will help in the detection and control of infections caused by
multidrug resistant P. aeruginosa.
The low susceptibility rate to aminoglycoside, piperacllin-tazobactam and
ciprofloxacin was found and in addition, detection of PmrB and arnA as
molecular markers in the follow up of infections caused by multidrug resistant P. aeruginosa. J Microbiol Infect Dis 2018; 8(2):61-68.

Anahtar Kelimeler

arnA, mcr-1, PmrB, Polymyxin, Pseudomonas aeruginosa, Multidrug resistance

Kaynakça

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