RNAemia and Clinical Outcome in COVID-19 Patients

Jyotsna Agarwal; Vikramjeet Singh; Jaya Garg; Ritu Karoli2; Swasti Tiwari; Shareh Naqvi; Anupam Das; Manodeep Sen

doi:10.5799/jmid.993839

RNAemia and Clinical Outcome in COVID-19 Patients

Abstract

Objectives: Objectives: SARS-CoV-2 virus dynamics in the human body and its correlation with disease severity and biomarkers have not been elucidated. This study aims to evaluate the viral load in the blood sample of COVID-19 patients and viral load association with disease severity and progression. Methods: Blood samples from COVID-19 positive inpatients were collected sequentially, at admission, and during hospitalization. Depending on the clinical presentation, the patients were classified into mild, moderate, and severe categories. The blood samples were subjected to SARS-CoV-2 Real-time polymerase chain reaction (RT-PCR); positive samples were then tested for viral load using digital drop RT-PCR. Patients were followed up until they were discharged. Results: Of 83 enrolled patients, 15 (18.1%) were mild, 45 (54%) were moderate, and 23 (27.7%) had severe symptoms. The blood PCR in 21 patients was positive (rate was 25.3); one was mild, four moderate, and 16 severe on the day of admission. The mean cycle threshold (CT) of RNAemia in these 21 patients was 24.7+3.1. The viral load was significantly higher in patients with severe symptoms than others. Serum ferritin, D-dimer, and plasma fibrinogen were found raised along with lymphopenia in all patients. Viremia was found persistently detectable (or even rising) in patients in the severe category and associated with a fatal outcome. Conclusion: A prolonged elevation of D-dimer, serum ferritin, and plasma fibrinogen together with lymphopenia lead to a greater risk of mortality and morbidity. Quantitative estimation of virus in blood has a clinical significance which may help in prognostication and management of patients of COVID-19. J Microbiol Infect Dis 2021; 11(3):116-123.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Jyotsna Agarwal Bu kişi benim
India

Vikramjeet Singh Bu kişi benim
India

Jaya Garg Bu kişi benim
India

Ritu Karoli2 Bu kişi benim
India

Swasti Tiwari Bu kişi benim
Palestine

Shareh Naqvi Bu kişi benim
India

Anupam Das Bu kişi benim
India

Manodeep Sen Bu kişi benim
India

Yayımlanma Tarihi

15 Eylül 2021

Gönderilme Tarihi

8 Şubat 2021

Kabul Tarihi

16 Ağustos 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 11 Sayı: 03

DOI

https://doi.org/10.5799/jmid.993839

IZ

https://izlik.org/JA93RG95AE

Kaynak Göster

RIS / Bibtex

APA

Agarwal, J., Singh, V., Garg, J., Karoli2, R., Tiwari, S., Naqvi, S., Das, A., & Sen, M. (2021). RNAemia and Clinical Outcome in COVID-19 Patients. Journal of Microbiology and Infectious Diseases, 11(03), 116-123. https://doi.org/10.5799/jmid.993839

AMA

1.Agarwal J, Singh V, Garg J, vd. RNAemia and Clinical Outcome in COVID-19 Patients. Journal of Microbiology and Infectious Diseases. 2021;11(03):116-123. doi:10.5799/jmid.993839

Chicago

Agarwal, Jyotsna, Vikramjeet Singh, Jaya Garg, vd. 2021. “RNAemia and Clinical Outcome in COVID-19 Patients”. Journal of Microbiology and Infectious Diseases 11 (03): 116-23. https://doi.org/10.5799/jmid.993839.

EndNote

Agarwal J, Singh V, Garg J, Karoli2 R, Tiwari S, Naqvi S, Das A, Sen M (01 Eylül 2021) RNAemia and Clinical Outcome in COVID-19 Patients. Journal of Microbiology and Infectious Diseases 11 03 116–123.

IEEE

[1]J. Agarwal vd., “RNAemia and Clinical Outcome in COVID-19 Patients”, Journal of Microbiology and Infectious Diseases, c. 11, sy 03, ss. 116–123, Eyl. 2021, doi: 10.5799/jmid.993839.

ISNAD

Agarwal, Jyotsna - Singh, Vikramjeet - Garg, Jaya - Karoli2, Ritu - Tiwari, Swasti - Naqvi, Shareh - Das, Anupam - Sen, Manodeep. “RNAemia and Clinical Outcome in COVID-19 Patients”. Journal of Microbiology and Infectious Diseases 11/03 (01 Eylül 2021): 116-123. https://doi.org/10.5799/jmid.993839.

JAMA

1.Agarwal J, Singh V, Garg J, Karoli2 R, Tiwari S, Naqvi S, Das A, Sen M. RNAemia and Clinical Outcome in COVID-19 Patients. Journal of Microbiology and Infectious Diseases. 2021;11:116–123.

MLA

Agarwal, Jyotsna, vd. “RNAemia and Clinical Outcome in COVID-19 Patients”. Journal of Microbiology and Infectious Diseases, c. 11, sy 03, Eylül 2021, ss. 116-23, doi:10.5799/jmid.993839.

Vancouver

1.Jyotsna Agarwal, Vikramjeet Singh, Jaya Garg, Ritu Karoli2, Swasti Tiwari, Shareh Naqvi, Anupam Das, Manodeep Sen. RNAemia and Clinical Outcome in COVID-19 Patients. Journal of Microbiology and Infectious Diseases. 01 Eylül 2021;11(03):116-23. doi:10.5799/jmid.993839