Yıl 2016, Cilt 6 , Sayı 4, Sayfalar 184 - 189 2016-12-01

Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis

Massoud Hajia HAJİA [1]


Objectives: The aim of this study was to analyze the isolated cholera strains at outbreak 2013 for studying the their similarity and compare their homology in order to find out the route of infection either emerge from abroad or reemerge from inside native strains. Methods: All diagnosed V. cholerae isolates were entered to the study after re-identification at referral laboratory of Health Ministry based on standard procedures. These specimens were examined for specific serogroups by O1 polyvalent and Ogawa/Inaba nonspecific antisera and tested by MIC Test Strip Method against Ciprofloxacin, Nalidixic Acid, Cefixime, Ampicillin, Tetracycline, Trimethoprim-Sulfamethoxazole, and Erythromycin. Results: A total of 257 clinical Vibrio cholerae was isolated in an outbreak of Iran at 2013. The dominant causative type was Inaba. In Antibiotic susceptibility test isolates were 100% resistant to all except Erythromycin that just 23% of strains were sensitive. Homology of isolates was investigated through genotyping by PFGE method and their clonality was compared with previous isolated Iranian native strain. Overall 92% of analyzed strains showed a homolog pattern. These strains were located in 8 clusters. Although isolated strains at 2011 had 80 % homology with recent isolates, located in complete distinct cluster than all strains isolated at 2013. PFGE analysis revealed no dissimilarity between those stains resistant and sensitive to Erythromycin. Conclusion: This study confirmed that isolated Inaba strains at 2013 had different clonality pattern in PFGE than previously identified, suggested have foreign route from the neighboring countries. J Microbiol Infect Dis 2016;6(4): 184-189  
Vibrio cholera, Outbreak, Inaba, PFGE
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Yazar: Massoud Hajia HAJİA

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Yayımlanma Tarihi : 1 Aralık 2016

Bibtex @araştırma makalesi { jmid328934, journal = {Journal of Microbiology and Infectious Diseases}, issn = {2146-3158}, eissn = {2146-9369}, address = {}, publisher = {Sağlık Araştırmaları Derneği}, year = {2016}, volume = {6}, pages = {184 - 189}, doi = {10.5799/jmid.328934}, title = {Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis}, key = {cite}, author = {HAJİA, Massoud Hajia} }
APA HAJİA, M . (2016). Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis. Journal of Microbiology and Infectious Diseases , 6 (4) , 184-189 . DOI: 10.5799/jmid.328934
MLA HAJİA, M . "Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis". Journal of Microbiology and Infectious Diseases 6 (2016 ): 184-189 <https://dergipark.org.tr/tr/pub/jmid/issue/30452/328934>
Chicago HAJİA, M . "Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis". Journal of Microbiology and Infectious Diseases 6 (2016 ): 184-189
RIS TY - JOUR T1 - Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis AU - Massoud Hajia HAJİA Y1 - 2016 PY - 2016 N1 - doi: 10.5799/jmid.328934 DO - 10.5799/jmid.328934 T2 - Journal of Microbiology and Infectious Diseases JF - Journal JO - JOR SP - 184 EP - 189 VL - 6 IS - 4 SN - 2146-3158-2146-9369 M3 - doi: 10.5799/jmid.328934 UR - https://doi.org/10.5799/jmid.328934 Y2 - 2017 ER -
EndNote %0 Journal of Microbiology and Infectious Diseases Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis %A Massoud Hajia HAJİA %T Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis %D 2016 %J Journal of Microbiology and Infectious Diseases %P 2146-3158-2146-9369 %V 6 %N 4 %R doi: 10.5799/jmid.328934 %U 10.5799/jmid.328934
ISNAD HAJİA, Massoud Hajia . "Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis". Journal of Microbiology and Infectious Diseases 6 / 4 (Aralık 2016): 184-189 . https://doi.org/10.5799/jmid.328934
AMA HAJİA M . Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis. J Microbil Infect Dis. 2016; 6(4): 184-189.
Vancouver HAJİA M . Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis. Journal of Microbiology and Infectious Diseases. 2016; 6(4): 189-184.