Objectives: The aim of this study was to analyze the isolated cholera strains at outbreak 2013 for studying the their
similarity and compare their homology in order to find out the route of infection either emerge from abroad or reemerge
from inside native strains.
Methods: All diagnosed V. cholerae isolates were entered to the study after re-identification at referral laboratory of
Health Ministry based on standard procedures. These specimens were examined for specific serogroups by O1 polyvalent
and Ogawa/Inaba nonspecific antisera and tested by MIC Test Strip Method against Ciprofloxacin, Nalidixic Acid,
Cefixime, Ampicillin, Tetracycline, Trimethoprim-Sulfamethoxazole, and Erythromycin.
Results: A total of 257 clinical Vibrio cholerae was isolated in an outbreak of Iran at 2013. The dominant causative type
was Inaba. In Antibiotic susceptibility test isolates were 100% resistant to all except Erythromycin that just 23% of strains
were sensitive. Homology of isolates was investigated through genotyping by PFGE method and their clonality was
compared with previous isolated Iranian native strain. Overall 92% of analyzed strains showed a homolog pattern. These
strains were located in 8 clusters. Although isolated strains at 2011 had 80 % homology with recent isolates, located in
complete distinct cluster than all strains isolated at 2013. PFGE analysis revealed no dissimilarity between those stains
resistant and sensitive to Erythromycin.
Conclusion: This study confirmed that isolated Inaba strains at 2013 had different clonality pattern in PFGE than previously
identified, suggested have foreign route from the neighboring countries. J Microbiol Infect Dis 2016;6(4): 184-189
Vibrio cholera, Outbreak, Inaba, PFGE