Typing of Methicillin-Resistant Staphylococcus aureus Isolate from Healthcare Workers in Larestan, Iran

Yıl 2018, Cilt: 08 Sayı: 01, 1 - 7, 15.03.2018

Mehdi Ebadi Hossein Ashrafi

https://doi.org/10.5799/jmid.393890

Cited By: 2

Öz

Objective:
The aim of this study was to determine the antimicrobial
susceptibility and cassette chromosome typing in Methicillin-Resistant Staphylococcus aureus (MRSA) isolated
from healthcare workers.

Methods:
230 nasal swabs were collected from healthcare workers of
three hospitals in Larestan, Iran. Antibiotic susceptibility was determined by
disk diffusion method according to CLSI guideline. The minimum Inhibitory
concentration (MIC) of vancomycin and screening test with methicillin were
measured by E-test and agar screen plate procedures. Cefoxitin disc diffusion
test was performed. MRSA isolates were selected and investigated for mecA gene and SCCmec typing by multiplex-PCR, then five methods were compared.

Results:
In total, 37 S.
aureus were isolated, 28 were defined as MRSA. Frequency of SCCmec types 9 SCCmec I, 8 SCCmec IV, 5 SCCmec II 4 SCCmec V 2 SCCmec III. In
this study, 75% of isolates were CA-MRSA,
25% were HA-MRSA. Evaluation of
antibiotic resistance showed the greatest resistance to penicillin and the
lowest resistance was observed to vancomycin and by E-test method 28.5% of
isolates were intermediate resistance to vancomycin. However, screening test
detected 92.8% resistance with oxacillin and 28 of isolates were resistant with
Cefoxitin disc diffusion. For these 28 isolates mecA was positive.

Conclusion:
As a result, in our S.
aureus isolates methicillin resistance was75.7%. The most frequent type was
SCCmec I. Our result showed high
rates of antibiotic resistance specially to methicillin in the S. aureus isolated hospitals that is a
serious warning to the treatment of infection caused by this bacterium. J Microbiol Infect Dis 2018; 8(1):1-7

Keywords:
Methicillin-resistant
Staphylococcus aureus, SCCmec, mecA

Anahtar Kelimeler

Methicillin-resistant Staphylococcus aureus, SCCmec, mecA

Kaynakça

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