Antifungal Susceptibility Pattern among Candida species: An Evaluation of Disc Diffusion and Micro Broth Dilution Method

Yıl 2018, Cilt: 08 Sayı: 03, 97 - 102, 15.09.2018

Abirami Lakshmy Jayachandran Radhika Katragadda Thyagarajan Ravinder Leela Vajravelu Lavanya Manorajan S. Hemalatha Kaliappan Shanmugam

https://doi.org/10.5799/jmid.458457

Cited By: 4

Öz

Objective: Candida species are emerging as a significant pathogen certain
species of Candida like Candida krusei
are inherently resistant to azoles. In vitro susceptibility testing is
essential for guiding therapy. The present study aims to study the antifungal
susceptibility pattern of Candida isolates by disc diffusion and micro broth
dilution method and to evaluate the degree of agreement between both the
techniques.

Methods: Candida
isolated from specimens like Oropharyngeal swabs, blood, pus and wound swabs
were included in the study. Speciation was done as per standard microbiological
methods. Antifungal resistance was determined by disc diffusion method for
fluconazole, Itraconazole, amphotericin B, nystatin and clotrimazole.
Microbroth dilution method was performed for fluconazole, Itraconazole and
amphotericin B. The degree of agreement between both the methods for the
resistant isolates was analysed by deriving the kappa value.

Results: Out
of the 156 Candida isolates obtained, Candida
albicans was the most common species isolated. By disc diffusion method
fluconazole and Itraconazole showed an overall resistance of 34 (21.7%) and 27
(17.3%), respectively. Using micro broth dilution method, Fluconazole and
Itraconazole had a resistance percentage of 35 (22.4%) and 29 (18.5%),
respectively. There was a good agreement between both the methods in detecting
the percentage of resistant isolates for fluconazole and Itraconazole (κ=0.9).

Conclusion: It is essential to perform susceptibility testing for
all the Candida isolates for providing crucial information about the resistance
pattern and help in choosing the appropriate antifungal drug for therapy. Disc
diffusion method which is easy to perform can be utilized for day to day
practice. J Microbiol Infect Dis 2018; 8(3):97-101

Anahtar Kelimeler

Broth micro dilution, Candida, Disc diffusion

Kaynakça

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