Akut Difenokonazol’ün Daphnia magna üzerindeki Genotoksik Etkileri

Öz

Difenokonazol, tarımda sıkça kullanılan bir fungisit olup; sucul canlılar için zararlı olabilmektedir. Difenokonazol’un sucul canlılarda olan toksisitesi henüz iyi bilinmemektedir. Triazol mantar ilaçları arasında, Difenokonazol (PEN) birçok ülkede en yaygın kullanılan fungusitlerden biridir. Bu çalışmada, Daphnia magna üzerinde farklı dozlarda Difenokonazolün genotoksik etkisi araştırıldı. Ayrıca Kontrol Grubu ve diğer doz grublarıyla yapılan çalışma, Ekonomik İşbirliği ve Kalkınma Örgütü (OECD) standartları 202 ve 212 (OECD, 2012, 2009) tarafından belirlenen standart metodolojiye uygun olarak gerçekleştirildi. Çalışmada, Daphnia magna 10 gün boyunca kontrol grubu (0 mg/L) ve üç farklı dozda Difenokonazole (1.00, 1.5, 2.5 mg/L) maruz bırakıldı. Deneyin sonunda, dokuların Hasar Frekansı (%), Arbitrary Birim (%), ve Genetik Hasar İndeksi (%) belirlemek için Comet testi kullanıldı. Doz gruplarından 2.5 mg L-1 grubu, Daphnia magna üzerinde en yüksek hasar frekansına sahip olduğu(45.33±1.52) (p<0.001) tespit edildi. Elde ettiğimiz bulguların Difenokonazol’un maruziyeti sonrasında Daphnia magna'da DNA hasar düzeylerinde önemli bir artış olduğunu göstermesine bağlı olarak kullanılan bu fungisitin daphnidler için genotoksik olabileceğini işaret etmiştir.

Anahtar Kelimeler

• pestisit
• Difenkonazol
• komet test
• DNA Hasarı

Genotoxic Effects of Acute Difenoconazole Exposure on Daphnia magna

Öz

Difenoconazole, a fungicide often used in agriculture, is harmful to aquatic organisms. However, its toxicity to aquatic organisms is not yet well recognized. Among the triazole fungicides, Difenoconazole (PEN) is one of the most extensively used in many countries. In this study, the genetic impacts of different Difenoconazole doses on Daphnia magna was investigated. Experiments involving the control group and treatments were executed in compliance with the standard methodology outlined by the Economic Cooperation and Development Organization (OECD) standards 202 and 212 (OECD, 2012, 2009). In the present study, Daphnia magna were exposed to a control group (0 mg/L) and three different doses of (1.00, 1.5, 2.5 mg/L) difenoconazole for a period of 10 days. At the end of the experiment, comet assay was used to determine the damage frequency (%), Arbitrary unit (%), and Genetic damage index (%) of tissues. The 2.5 mg L-1 group showed notably greater damage frequencies (45.33±1.52) on Daphnia magna (p<0.001). Our findings indicated a considerable increase in DNA strand breakage in Daphnia magna after exposure to difenoconazole, indicating that the fungicide is genotoxic to daphnids.

Anahtar Kelimeler

• Pesticide,
• Difenoconazole,
• Comet Assay
• DNA Damage

Kaynakça

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