Protective Effect of Taxifolin in The Prevention of Cardiac Tissue Damage in Liver Ischemia and Reperfusion Injury: Experimental Study

Abstract

Aim: Liver ischemia and reperfusion (I/R) is a serious, irreversible health problem in clinical practice. Taxifolin (Tax) is an easy to obtain and use agent found in maritime pine bark, Douglas fir bark and Siberian larch wood. In this study, we examined the protective efficacy of Taxifolin in the correction of cardiac tissue damage that may develop in liver I/R damage. Methods: In our study, a total of 28 Wistar Albino rats, 8-10 weeks old, weighing 250-300 grams, were used. Group 1 (n=7): control group, Group 2 (n=7): Tax group with 50 mg/kg dose orally for 3 weeks, Group 3 (n=7): Liver I/R group for 30 minutes ischemia and 120 minutes of reperfusion were performed. Group 4 (n=7): Tax+Liver I/R group. Results: In our study, MDA analysis was performed to evaluate oxidative stress. In the statistical analysis of MDA values, we observed that there was a statistically significant difference between the serum MDA values of the Tax group and the Tax+Liver I/R group, and the MDA level of the Tax group was lower (p<0.05). In myocyte damage scoring, we observed that the liver I/R group had the highest damage score, while the damage score of the Tax+Liver I/R group was significantly lower than the I/R group (p<0.05). Conclusion: As a result of our study, we observed that there was an increase in serum MDA levels as a result of liver I/R and histopathological changes occurred in the heart tissue. However, Taxifolin has been successful in ameliorating this situation.

Keywords

• Taxifolin
• Oxidative Stress
• Liver ischemia and reperfusion
• Heart

KARACİĞER İSKEMİ VE REPERFÜZYON HASARINDA KALP DOKUSUNDA MEYDANA GELEN HASARIN ÖNLENMESİNDE TAKSİFOLİN’İN KORUYUCU ETKİNLİĞİ: DENEYSEL ÇALIŞMA

Öz

Amaç: Karaciğer iskemi ve reperfüzyon (I/R) klinik uygulamalarında geri dönüşümsüz ciddi bir sağlık sorunudur. Taksifolin (Tax) deniz çamı kabuğunda, Douglas köknar kabuğunda ve Sibirya karaçam ağacında, bulunan elde edilmesi ve kullanılması kolay bir ajandır. Bizde yaptığımız bu çalışmanın sonucunda karaciğer I/R hasarında gelişebilecek kalp dokusu hasarının düzeltilmesinde Taksifolin’in koruyucu etkinliğini incelemeye karar verdik. Yöntemler: Çalışmamızda ortalama 8-10 haftalık, 250-300 gram ağırlıklarında toplam 28 adet Wistar Albino cinsi ratlar kullanıldı. Grup 1 (n=7): kontrol grubu, Grup 2 (n=7): Tax grubu olup 50 mg/kg doz ile oral yoldan 3 hafta süreyle, Grup 3 (n=7): Karaciğer I/R grubunda ise 30 dakika iskemi ve 120 dakika reperfüzyon uygulandı. Grup 4 (n=7): Tax+Karaciğer I/R grubu idi. Bulgular: Çalışmamızda oksidatif stresi değerlendirmek için MDA analizi yapıldı. MDA değerlerinin istatistiksel analizinde; Tax grubu ile Tax+Karaciğer I/R grubunun serum MDA değerleri arasında istatistiksel olarak anlamlı fark olduğu, Tax grubunun MDA düzeyinin daha düşük olduğu görüldü (p<0.05). Myosit hasarı skorlamasında; en yüksek hasar skorlamasının karaciğer I/R grubunda olduğu izlenirken, Tax+Karaciğer I/R grubunun hasar skorlamasının I/R grubundan anlamlı olarak daha düşük olduğu izlendi (p<0.05). Sonuç: Çalışmamızın sonucunda karaciğer I/R sonucu serum MDA düzeylerinde artış olduğu ve kalp dokusunda histopatolojik değişikliklerin meydana geldiği görülmüştür. Fakat bu durumun düzeltilmesinde Taksifolin başarılı şekilde etkinlik göstermiştir

Anahtar Kelimeler

• Taksifolin
• Oksidatif Stres
• Karaciğer iskemi ve reperfüzyon
• Kalp

Kaynakça

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