KARACİĞER İSKEMİ VE REPERFÜZYONA BAĞLI BÖBREK HASARLARININ ÖNLENMESİNDE TAKSİFOLİN’İN ETKİNLİĞİ

Öz

Amaç: Karaciğer rezeksiyonu ve transplantasyonu gibi cerrahi işlemler sırasında yaklaşık %10 gibi bir oranda iskemi/reperfüzyon (I/R) hasarı gibi ciddi komplikasyonlar görülebilmektedir. Bu çalışmada böylesi bir komplikasyonun karaciğer dışında böbrek dokusu gibi bir organda meydana getireceği hasarın düzeltilmesinde Taksifolin’in etkinliğini histolojik ve biyokimyasal açıdan incelemeyi planladık. Yöntem: Çalışmamızda ortalama 8-10 haftalık, 250-300 gram ağırlıklarında toplam 28 adet Wistar Albino cinsi ratlar kullandı. Grup 1 (n=7): kontrol grubu, Grup 2 (n=7): Taksifolin grubu olup 50 mg/kg doz ile oral yoldan 3 hafta süreyle, Grup 3 (n=7): Karaciğer I/R grubunda ise 30 dakika iskemi ve 120 dakika reperfüzyon uygulandı. Grup 4 (n=7): Taksifolin+Karaciğer I/R grubu idi. Bulgular: Çalışmamızın histolojik bulgularında karaciğer I/R uygulanan grubun böbrek dokularına atrofi, tübül epitelerinde dejenerasyon ve TNF-α ekspresyonlarında artış izlendi. ayrıca bu grubun böbrek fonksiyon testlerinde de bozulmalar izlendi. Tax+Karaciğer I/R grubunda ise, Karaciğer I/R grubuna kıyasla hem histolojik hemde biyokimyasal bazda anlamlı fark olduğu ve olumlu yönde etkilendiği gözlendi (p<0.05). Sonuç: Karaciğer iskemi ve reperfüzyonu sonucu böbrek dokularının fonksiyonlarında ve histolojik görünümlerinde bozulmalar izlenirken, oluşan bu olumsuz etkilerin giderilmesinde Taksifolin’in etkin olduğu görüldü.

Anahtar Kelimeler

• Karaciğer iskemi ve reperfüzyon
• Taksifolin
• Histopatoloji
• İmmunohistokimya
• Oksidatif stres

Efficacy of Taxifolin in The Prevention of Renal Injury Due to Liver Ischemia and Reperfusion

Abstract

Aim: During surgical procedures such as liver resection and transplantation, ischemia/reperfusion (I/R) injury and related complications may occur at a rate of approximately 10%. Our study, we planned to investigate histologically and biochemically the efficacy of Taxifolin in the prevention of renal tissue damage in liver ischemia reperfusion. Methods: A total of 28 Wistar Albino rats with an average age of 8-10 weeks and weights of 250-300 grams were used in our study. Group 1 (n=7): control group, Group 2 (n=7): Taxifolin group; Taxifolin was administered orally at a dose of 50 mg/kg for 3 weeks, Group 3 (n=7): Liver I/R group, 30 minutes ischemia and 120 minutes reperfusion was performed. Group 4 (n=7): Taxifolin+Liver I/R group. Results: Kidney tissues of the liver I/R group showed atrophy, degeneration of tubule epithelium and increased TNF-α expression. In addition, deterioration in renal function tests was also monitored in this group. In the Taxifolin+Liver I/R group, a significant difference was observed on both histologic and biochemical basis compared to the Liver I/R group and a positive effect was observed (p<0.05). Outcome: As a consequence of hepatic ischemia and reperfusion, impairment in the function and histological appearance of renal tissues was observed and Taxifolin was monitored to be effective in eliminating these adverse effects.

Keywords

• liver ischemia and reperfusion
• taxifolin
• histopathology
• immunohistochemistry
• oxidative stress

Kaynakça

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