Effect of Biochanin-A on The Cerebellum in Cerebral Ischemia Reperfusion Injury

Yıl 2024, , 47 - 51, 11.03.2024

Öner Avınca , Özge Kaplan , Fırat Aşır

https://doi.org/10.36516/jocass.1425526

Öz

ABSTRACT

Objective: To examine the protective effect of Biochanin A (BCA) on the cerebellum in cerebral ischemia reperfusion injury.
Material and Metodhs: 24 Wistar albino female rats were divided into 3 groups. Control, Ischemia reperfusion (IR), IR +BCA (20 mg/kg intraperitoneal) group. A micro bulldog clamp was placed on the left common carotid artery of the rats and cerebral ischemia was applied for 2 hours. After cerebral ischemia, the clamp was removed and reperfusion was performed for 24 hours. After 7 days, all rats were decapitated and the protective effects of Biochanin A on the cerebellum were evaluated by immunohistochemically analyses.
Results: In the IR group; S100 expression was also observed positive in neurons and neuroglia in the pia mater, molecular layer, Purkinje cells in the ganglionic layer and granule cells in the granular layer, and neuroglia in the white matter. In the IR+ Biochanin A group, it was observed that the immune activity in the granule cells and Purkinje cells in the granular layer was reduced compared to the IR group.
Conclusion: We suggest that Biochanin A treatment has a potential therapeutic role in alleviating inflammation in the cerebellum after cerebral ischemia reperfusion.

Anahtar Kelimeler

Cerebral ischemia, Biochanin A, S100

SEREBRAL İSKEMİ REPERFÜZYON HASARINDA BİYOKANİN-A'NIN SEREBELLUM ÜZERİNDEKİ ETKİSİ

Öz

ÖZET

Amaç:Serebral iskemi reperfüzyon hasarında Biochanin A'nın (BCA) serebellum üzerindeki koruyucu etkisini incelemek.
Materyal ve Metod: 24 Wistar albino dişi sıçan 3 gruba ayrıldı. Kontrol, İskemi reperfüzyon (İR), İR +BCA (20 mg/kg intraperitoneal) grubu. Sıçanların sol ana karotid arterine bir mikro bulldog klemp yerleştirildi ve 2 saat boyunca serebral iskemi uygulandı. Serebral iskemi sonrasında klemp çıkarıldı ve 24 saat boyunca reperfüzyon uygulandı. 7 gün sonra tüm sıçanlar dekapite edildi ve Biochanin A'nın serebellum üzerindeki koruyucu etkileri immünohistokimyasal analizlerle değerlendirildi.
Bulgular: IR grubunda; pia mater, moleküler tabaka, ganglionik tabakada Purkinje hücreleri ve granüler tabakada granül hücreleri ve beyaz cevherde nörogliadaki nöron ve nöroglialarda S100 ekspresyonu pozitif olarak gözlendi. IR+ Biyokanin A grubunda, granül hücrelerdeki ve granüler tabakadaki Purkinje hücrelerindeki immün aktivitenin IR grubuna kıyasla azaldığı gözlenmiştir.
Sonuç: Biochanin A tedavisinin serebral iskemi reperfüzyonundan sonra serebellumdaki enflamasyonu hafifletmede potansiyel bir terapötik rolü olduğunu düşünüyoruz.

Anahtar Kelimeler

Serebral iskemi, Biyokanin A, S100

Kaynakça

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