Effects of Curcumin and Doxorubicin on the Viability of Neuroblastoma Cancer Cell Line

Yıl 2024, , 191 - 194, 30.09.2024

İlker Ünlü , İlhan Özdemir , Şamil Öztürk , M. Cudi Tuncer

https://doi.org/10.36516/jocass.1520583

Öz

Aim: Neuroblastoma has a very important place among childhood diseases, and despite all the methods used in treatment, it is very difficult to prevent neuroblastoma invasion. The number of studies showing that curcumin, the most active component of turmeric, is not toxic, is increasing day by day. In this study, the anticancer activities of curcumin (Cur), one of the important active compounds, were demonstrated in the Neuroblastoma cancer cell line (Na2B).
Method: Neuroblastoma cell line was used in the study. To determine the IC50 doses of Dox and Cur, Na2B cells line were cultivated with an automatic pipette. MTT analysis was performed to analyze cell survival (viability). Inhibition levels in the cells were determined at 24 and 48 hours.
Results: While the IC50 of Na2B cell proliferation was approximately 124.5 uM at the 48th hour in Dox-treated cells, the IC50 value of Cur at the 48th hour was found to be 224.6 uM.
Conclusion: These results showed that Cur could be an alternative agent in the treatment of neuroblastoma, and its fewer side effects compared to other chemotherapeutic agents such as Dox would increase its preferability.

Anahtar Kelimeler

Neuroblastoma, Na2B cell line, cancer, MTT, curcumin

Etik Beyan

Sayın Editör, Çalışmamız in vitro yapıldığı için etik onay gerektirmemektedir.

Kaynakça

• 1.Nakazawa A. Biological categories of neuroblastoma based on the international neuroblastoma pathology classification for treatment stratification. Pathol Int. 2021;71(4):232-44. https://doi.org/10.1111/pin.13085
• 2.Muller AG, Sarker SD, Saleem IY, et al. Delivery of natural phenolic compounds for the potential treatment of lung cancer. Daru. 2019;27(1):433-49. https://doi.org/10.1007/s40199-019-00267-2
• 3.Peng Y, Ao M, Dong B, et al. Anti-Inflammatory Effects of Curcumin in the Inflammatory Diseases: Status, Limitations and Countermeasures. Drug Des Devel Ther. 2021;15:4503-25. https://doi.org/10.2147/DDDT.S327378
• 4.Mansouri K, Rasoulpoor S, Daneshkhah A, et al. Clinical effects of curcumin in enhancing cancer therapy: A systematic review. BMC Cancer. 2020;20(1):791. https://doi.org/10.1186/s12885-020-07256-8
• 5.Khan AQ, Ahmed EI, Elareer N, et al. Curcumin-Mediated Apoptotic Cell Death in Papillary Thyroid Cancer and Cancer Stem-Like Cells through Targeting of the JAK/STAT3 Signaling Pathway. Int J Mol Sci. 2020;21(2):438. https://doi.org/10.3390/ijms21020438
• 6.Sohn SI, Priya A, Balasubramaniam B, et al. Biomedical Applications and Bioavailability of Curcumin-An Updated Overview. Pharmaceutics. 2021;13(12):2102. https://doi.org/10.3390/pharmaceutics13122102
• 7.Aborehab NM, Osama N. Effect of Gallic acid in potentiating chemotherapeutic effect of Paclitaxel in HeLa cervical cancer cells. Cancer Cell Int. 2019;19:154. https://doi.org/10.1186/s12935-019-0868-0
• 8.Gong C, Xie Y, Zhao Y, et al. Comparison of two regimens of weekly paclitaxel plus gemcitabine in patients with metastatic breast cancer: propensity score-matched analysis of real-world data. Ther Adv Drug Saf. 2022;13:20420986221146411. https://doi.org/10.1177/20420986221146411
• 9.Bayat Mokhtari R, Baluch N, Morgatskaya E, et al. Human bronchial carcinoid tumor initiating cells are targeted by the combination of acetazolamide and sulforaphane. BMC Cancer. 2019;19(1):864. https://doi.org/10.1186/s12885-019-6018-1
• 10.Frazier AL, Stoneham S, Rodriguez-Galindo C, et al. Comparison of carboplatin versus cisplatin in the treatment of paediatric extracranial malignant germ cell tumours: a report of the Malignant Germ Cell International Consortium. Eur J Cancer. 2018;98:30-7. https://doi.org/10.1016/j.ejca.2018.03.004
• 11.Jiang S, Pan AW, Lin TY, et al. Paclitaxel Enhances Carboplatin-DNA Adduct Formation and Cytotoxicity. Chem Res Toxicol. 2015;28(12):2250-2. https://doi.org/10.1021/acs.chemrestox.5b00422
• 12.Şen B, Toros P, Sönmez PK, et al. The Effect of Herbal Medicine on Neuroblastoma Cell Line in Culture. Proceedings. 2017;1(10):1012. https://doi.org/10.3390/proceedings1101012
• 13.Sönmez PK, Albayrak G, Özkut M, et al. Antiproliferative and Apoptotic Effects of the Medicinal Plants on Breast Cancer Cell Lines. Proceedings. 2017; 1(10):1021. https://doi.org/10.3390/proceedings1101021
• 14.Prathipati P, Pathania AS, Chaturvedi NK, et al. SAP30, an oncogenic driver of progression, poor survival, and drug resistance in neuroblastoma. Mol Ther Nucleic Acids. 2022;35(2):101543. https://doi.org/10.1016/j.omtn.2022.03.014
• 15.Fidler IJ, Kripke ML. The challenge of targeting metastasis. Cancer Metastasis Rev. 2015;34(4):635-41. https://doi.org/10.1007/s10555-015-9586-9
• 16.Baston C, Parosanu AI, Mihai M, et al. Tumor-to-Tumor Metastasis of Lung Cancer to Kidney Cancer: A Review of the Literature and Our Experience. Diagnostics (Basel). 2024;14(5):553. https://doi.org/10.3390/diagnostics14050553
• 17.Calabriso N, Massaro M, Scoditti E, et al. Red Grape Skin Polyphenols Blunt Matrix Metalloproteinase-2 and -9 Activity and Expression in Cell Models of Vascular Inflammation: Protective Role in Degenerative and Inflammatory Diseases. Molecules. 2016;21(9):1147. https://doi.org/10.3390/molecules21091147
• 18.Yang ZJ, Huang SY, Zhou DD, et al. Effects and Mechanisms of Curcumin for the Prevention and Management of Cancers: An Updated Review. Antioxidants (Basel). 2022;11(8):1481. https://doi.org/10.3390/antiox11081481
• 19.Kabir MT, Rahman MH, Akter R, et al. Potential Role of Curcumin and Its Nanoformulations to Treat Various Types of Cancers. Biomolecules. 2021;11(3):392. https://doi.org/10.3390/biom11030392,
• 20.Joshi P, Verma K, Kumar Semwal D, et al. Mechanism insights of curcumin and its analogues in cancer: An update. Phytother Res. 2023 Dec;37(12):5435-63. https://doi.org/10.1002/ptr.7983
• 21.Macharia JM, Mwangi RW, Rozmann N, Zsolt K, Varjas T, Uchechukwu PO, Wagara IN, Raposa BL. Medicinal plants with anti-colorectal cancer bioactive compounds: Potential game-changers in colorectal cancer management. Biomed Pharmacother. 2022;153:113383. https://doi.org/10.1016/j.biopha.2022.113383
• 22.González-Rodríguez M, Ait Edjoudi D, Cordero-Barreal A, et al. Oleocanthal, an Antioxidant Phenolic Compound in Extra Virgin Olive Oil (EVOO): A Comprehensive Systematic Review of Its Potential in Inflammation and Cancer. Antioxidants (Basel). 2023;12(12):2112. https://doi.org/10.3390/antiox12122112
• 23.Olgierd B, Kamila Ż, Anna B, et al. The Pluripotent Activities of Caffeic Acid Phenethyl Ester. Molecules. 2021;26(5):1335. https://doi.org/10.3390/molecules26051335
• 24.Varnamkhasti TJ, Jafarzadeh M, Sadeghizadeh M, et al. Radiosensitizing effect of dendrosomal nanoformulation of curcumin on cancer cells. Pharmacol Rep. 2022;74(4):718-735. https://doi.org/10.1007/s43440-022-00383-y
• 25.Mortazavi Farsani SS, Sadeghizadeh D, Babashah S, et al. The Involvement of Canonical NFκB Pathway in Megakaryocyte Differentiation Induction by Nanocurcumin. Int J Hematol Oncol Stem Cell Res. 2023;17(1):18-27. https://doi.org/10.18502/ijhoscr.v17i1.11709
• 26.Eslami SS, Jafari D, Ghotaslou A, et al. Combined Treatment of Dendrosomal-Curcumin and Daunorubicin Synergistically Inhibit Cell Proliferation, Migration and Induce Apoptosis in A549 Lung Cancer Cells. Adv Pharm Bull. 2023;13(3):539-550.