Term ve Preterm Prematüre Membran Rüptürü Olan Plasentalarda IL-6 Ekspresyonunun Araştırılması

Öz

Amaç: Bu çalışmada, preterm ve term prematüre membran rüptürü (EMR) tanısı almış hastaların plasentalarındaki IL-6 ekspresyonu immünohistokimyasal ve in silico analiz yoluyla araştırılmıştır. Gereç ve Yöntemler: XXX Üniversitesi Kadın Hastalıkları ve Doğum Kliniği'nde doğum yapan 40 sağlıklı hasta ve 40 preterm ve 40 term EMR tanısı almış hastanın plasentaları histolojik doku gömme protokolüne tabi tutulmuştur. Dokularda IL-6 immün boyama yapılmıştır. IL-6'nın EMR’deki rolünün altında yatan olası moleküler mekanizmaları araştırmak için biyoinformatik analiz yapılmıştır. Sonuç: IL-6 ekspresyonu kontrol grubunda genel olarak negatifti ancak hem preterm EMR hem de term EMR gruplarında anlamlı olarak daha yüksekti ancak preterm ve term PROM grupları arasında anlamlı bir fark yoktu. Preterm EMR grubunda, IL-6 sitotrofoblast, sinsitiyotrofoblast ve fibrinoid birikimi olan alanlarda pozitif olarak kaydedildi ancak villus bağ dokusu hücrelerinde ve vasküler endotelde negatifti. EMR grubunda, trofoblastik tabaka, sinsitiyal düğümler ve fibrinoid birikiminde yoğun IL-6 ekspresyonu gözlendi, ancak villöz stromal ve vasküler endotel hücrelerinde genellikle negatifti. IL-6 ile ilişkili potansiyel EMR hedeflerinin zenginleştirme analizi, lipopolisakkarit, bakteri kökenli molekül ve biyotik uyaranlara hücresel yanıt gibi mikrobiyal yanıtlarla ilgili önemli yollar ortaya koydu ve IL-6'nın EMR’yi baskın olarak bu yollar aracılığıyla modüle edebileceğini gösterdi. Sonuç: Kontrol grubuyla karşılaştırıldığında, preterm PROM ve term PROM gruplarında önemli histopatolojik değişiklikler ve artmış IL-6 ekspresyonu gözlendi ve in-silico analiz, EMR’nin IL-6 yoluyla plasenta iltihabını ve hasarını artırdığını gösterdi.

Anahtar Kelimeler

• Prematüre Membran Rüptürü
• Terim
• Preterm
• histopatoloji
• IL-6

Investigation of IL-6 Expression in Placentas with Term and Preterm Premature Membrane Rupture

Abstract

Aim: This study investigated IL-6 expression in placentas of patients diagnosed with preterm and term premature rupture of membranes (PROM) via immunohistochemical and in silico analysis. Materials and Methods: Placentas of 40 healthy patients and 40 preterm and 40 term patients diagnosed with term PROM who gave birth in Gynecology and Obstetrics Clinic of XXX University were subjected to histological tissue embedding protocol. IL-6 immunostaining was performed on tissues. Bioinformatic analysis was performed to explore the potential molecular mechanisms underlying role of IL-6 role in PROM. Results: IL-6 expression was generally negative in control group however significantly higher in both preterm PROM and term PROM groups, but no significant difference in between preterm and term PROM groups. In preterm PROM group, IL-6 was recorded as positive in areas with cytotrophoblast, syncytiotrophoblast and fibrinoid accumulation, but was negative in villus connective tissue cells and vascular endothelium. In EMR group, intense IL-6 expression was observed in trophoblastic layer, syncytial nodes and fibrinoid accumulation, but was generally negative in villous stromal and vascular endothelial cells. Enrichment analysis of IL-6-associated potential PROM targets revealed significant pathways related to microbial responses such as cellular response to lipopolysaccharide, molecule of bacterial origin and biotic stimuli, indicating that IL-6 may modulate PROM predominantly through these pathways. Conclusion: Compared to control group, significant histopathological changes and increased IL-6 expression were observed in preterm PROM and term PROM groups, with in-silico analysis, indicating PROM increased placental inflammation and damage via IL-6.

Keywords

• premature rupture of membrane
• term
• preterm
• histopathology
• IL-6

Kaynakça

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