Kronik Parsiyel Üreter Obstrüksiyonu Olan Tek Böbrekli Sıçanlarda Akut Dehidratasyonun Böbrek Üzerine Etkileri

Öz

Amaç: Bu çalışmada, deneysel olarak oluşturulmuş parsiyel üreter obstrüksiyonu bulunan tek böbrekli sıçanlarda dehidratasyonun böbrek üzerindeki etkileri değerlendirildi. Gereç ve Yöntemler: Yirmi adet erkek Wistar albino sıçan, her biri beş hayvandan oluşan dört gruba rastgele ayrıldı: Grup 1, sağ nefrektomi (kontrol); Grup 2, sağ nefrektomi + dehidratasyon; Grup 3, sağ nefrektomi + sol parsiyel üreter obstrüksiyonu; Grup 4, sağ nefrektomi + sol parsiyel üreter obstrüksiyonu + dehidratasyon. Kronik parsiyel üreter obstrüksiyonu, sol üreterin dört hafta süreyle psoas kası içine yerleştirilmesiyle oluşturuldu. Dehidratasyon, 48 saatlik su kısıtlaması ile sağlandı. Bulgular: Hematokrit, idrar yoğunluğu ve kan üre düzeyleri dehidratasyon uygulanan gruplarda anlamlı olarak artarken, kan kreatinin düzeyi yalnızca Grup 4’te anlamlı şekilde yükseldi (p=0.01). Sintigrafik değerlendirme, glomerüler filtrasyon hızının tüm deney gruplarında azaldığını ve en düşük değerin Grup 4’te olduğunu gösterdi (p=0.01). Tüm deney gruplarında kontrol grubuna kıyasla Tmax ve renal perfüzyon süresinde anlamlı uzama ve renal tutulumda azalma izlendi. Bu değişiklikler en belirgin olarak Grup 4’te gözlendi (p=0.01). Histopatolojik incelemede papiller ve tübüler deformiteler, granülosit birikimi ve medüller ödem gibi bulguların Grup 4’teki örneklerde diğer gruplara göre daha belirgin olduğu görüldü. Sonuç: Tek böbrekli sıçanlarda kronik parsiyel üreter obstrüksiyonu, böbreğin yapı ve fonksiyonunu olumsuz etkilemiş; buna akut dehidratasyonun eklenmesi ise bu hasarı daha da şiddetlendirmiştir.

Anahtar Kelimeler

• Kısmi üreter tıkanıklığı
• dehidratasyon
• böbrek fonksiyonu
• histopatoloji
• böbrek

Effects of Acute Dehydration on the Kidney in Solitary Kidney Rats With Chronic Partial Ureteral Obstruction

Abstract

Aim: In this study, we evaluated the effects of dehydration on the kidney in rats with a single kidney subjected to experimentally-induced partial ureteral obstruction. Materials and Methods: Twenty male Wistar albino rats were randomly assigned to four groups of five animals each: Group 1, right nephrectomy (control); Group 2, right nephrectomy + dehydration; Group 3, right nephrectomy + left partial ureteral obstruction; and Group 4, right nephrectomy + left partial ureteral obstruction + dehydration. Chronic partial ureteral obstruction was induced by embedding the left ureter into the psoas muscle for four weeks. Dehydration was achieved via 48-hour water restriction. Results: Hematocrit, urine density, and blood urea levels were significantly increased in the dehydration groups, whereas blood creatinine was significantly elevated only in Group 4 (p=0.01). Scintigraphic evaluation demonstrated that glomerular filtration rate decreased in all experimental groups, with the lowest value observed in Group 4 (p=0.01). All experimental groups showed significant prolongation of Tmax and renal perfusion time and a decrease in renal uptake compared to the control group. These changes were most pronounced in Group 4 (p=0.01). Histopathological examination revealed that alterations such as papillary and tubular deformities, granulocyte accumulation, and medullary edema were more pronounced in rat samples from Group 4 compared to the other groups. Conclusion: Chronic partial ureteral obstruction in rats with a single kidney adversely affected renal structure and function, and the addition of acute dehydration further exacerbated this damage.

Keywords

• Partial ureteral obstruction
• dehydration
• renal function
• histopathology
• kidney

Kaynakça

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