Kronik Parsiyel Üreter Obstrüksiyonu Olan Tek Böbrekli Sıçanlarda Akut Dehidratasyonun Böbrek Üzerine Etkileri

Yıl 2025, Cilt: 8 Sayı: 4, 519 - 523, 31.12.2025

Aziz Serhat Baykara , Mete Kaya , Çiğdem Arslan Alıcı

https://doi.org/10.36516/jocass.1831678

https://izlik.org/JA89ET85ZM

Öz

Amaç: Bu çalışmada, deneysel olarak oluşturulmuş parsiyel üreter obstrüksiyonu bulunan tek böbrekli sıçanlarda dehidratasyonun böbrek üzerindeki etkileri değerlendirildi.
Gereç ve Yöntemler: Yirmi adet erkek Wistar albino sıçan, her biri beş hayvandan oluşan dört gruba rastgele ayrıldı: Grup 1, sağ nefrektomi (kontrol); Grup 2, sağ nefrektomi + dehidratasyon; Grup 3, sağ nefrektomi + sol parsiyel üreter obstrüksiyonu; Grup 4, sağ nefrektomi + sol parsiyel üreter obstrüksiyonu + dehidratasyon. Kronik parsiyel üreter obstrüksiyonu, sol üreterin dört hafta süreyle psoas kası içine yerleştirilmesiyle oluşturuldu. Dehidratasyon, 48 saatlik su kısıtlaması ile sağlandı.
Bulgular: Hematokrit, idrar yoğunluğu ve kan üre düzeyleri dehidratasyon uygulanan gruplarda anlamlı olarak artarken, kan kreatinin düzeyi yalnızca Grup 4’te anlamlı şekilde yükseldi (p=0.01). Sintigrafik değerlendirme, glomerüler filtrasyon hızının tüm deney gruplarında azaldığını ve en düşük değerin Grup 4’te olduğunu gösterdi (p=0.01). Tüm deney gruplarında kontrol grubuna kıyasla Tmax ve renal perfüzyon süresinde anlamlı uzama ve renal tutulumda azalma izlendi. Bu değişiklikler en belirgin olarak Grup 4’te gözlendi (p=0.01). Histopatolojik incelemede papiller ve tübüler deformiteler, granülosit birikimi ve medüller ödem gibi bulguların Grup 4’teki örneklerde diğer gruplara göre daha belirgin olduğu görüldü.
Sonuç: Tek böbrekli sıçanlarda kronik parsiyel üreter obstrüksiyonu, böbreğin yapı ve fonksiyonunu olumsuz etkilemiş; buna akut dehidratasyonun eklenmesi ise bu hasarı daha da şiddetlendirmiştir.

Anahtar Kelimeler

Kısmi üreter tıkanıklığı , dehidratasyon , böbrek fonksiyonu , histopatoloji , böbrek

Etik Beyan

Çalışma, Harran Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu tarafından onaylanmış ve Harran Üniversitesi Bilimsel Araştırma Projeleri Ofisi tarafından desteklenmiştir (2002/9). Tüm hayvan prosedürleri Laboratuvar Hayvanlarının Bakımı ve Kullanımı Kılavuzu'na uygun olarak gerçekleştirilmiş ve ARRIVE yönergelerine uyulmuştur.

Destekleyen Kurum

Harran Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

2002/9

Teşekkür

Çalışmamızın sintigrafik analizlerine katkılarından dolayı Dr. Ahmet Kara’ya ve patolojik inceleme ve analizlerdeki desteklerinden dolayı Dr. Hasan İlyas Özardalı’ya teşekkür ederiz.

Effects of Acute Dehydration on the Kidney in Solitary Kidney Rats With Chronic Partial Ureteral Obstruction

https://izlik.org/JA89ET85ZM

Öz

Aim: In this study, we evaluated the effects of dehydration on the kidney in rats with a single kidney subjected to experimentally-induced partial ureteral obstruction.
Materials and Methods: Twenty male Wistar albino rats were randomly assigned to four groups of five animals each: Group 1, right nephrectomy (control); Group 2, right nephrectomy + dehydration; Group 3, right nephrectomy + left partial ureteral obstruction; and Group 4, right nephrectomy + left partial ureteral obstruction + dehydration. Chronic partial ureteral obstruction was induced by embedding the left ureter into the psoas muscle for four weeks. Dehydration was achieved via 48-hour water restriction.
Results: Hematocrit, urine density, and blood urea levels were significantly increased in the dehydration groups, whereas blood creatinine was significantly elevated only in Group 4 (p=0.01). Scintigraphic evaluation demonstrated that glomerular filtration rate decreased in all experimental groups, with the lowest value observed in Group 4 (p=0.01). All experimental groups showed significant prolongation of Tmax and renal perfusion time and a decrease in renal uptake compared to the control group. These changes were most pronounced in Group 4 (p=0.01). Histopathological examination revealed that alterations such as papillary and tubular deformities, granulocyte accumulation, and medullary edema were more pronounced in rat samples from Group 4 compared to the other groups.
Conclusion: Chronic partial ureteral obstruction in rats with a single kidney adversely affected renal structure and function, and the addition of acute dehydration further exacerbated this damage.

Anahtar Kelimeler

Partial ureteral obstruction , dehydration , renal function , histopathology , kidney

Etik Beyan

The study was approved by the Local Ethics Committee for Animal Experiments of Harran University and was supported by the Harran University Scientific Research Projects Office (2002/9). All animal procedures were performed in accordance with the Guide for the Care and Use of Laboratory Animals and adhered to the ARRIVE guidelines.

Destekleyen Kurum

Harran University Scientific Research Projects Office

Proje Numarası

2002/9

Teşekkür

We would like to thank Nuclear Medicine Specialist Dr. Ahmet Kara for his contributions to the scintigraphic analysis of our study and Pathology Specialist Dr. Hasan İlyas Özardalı for his support in the pathological examinations and analyses.

Kaynakça

• 1.Manjunath AS, Hofer MD. Urological emergencies. Med Clin North Am. 2018;102(2):373-385. [Crossref]
• 2.Rishor-Olney CR, Hinson MR. Obstructive uropathy. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan- [updated 2022 Aug 22].
• 3.La Scola C, Ammenti A, Bertulli C, Bodria M, Brugnara M, Camilla R, et al. Management of the congenital solitary kidney: consensus recommendations of the Italian Society of Pediatric Nephrology. Pediatr Nephrol. 2022;37(9):2185-2207. [Crossref]
• 4.Sancak EB, Turkon H, Silan C. Attenuation of partial unilateral ureteral obstruction-induced renal damage with hyperbaric oxygen therapy in a rat model. Int Braz J Urol. 2017;43(5):946-956. [Crossref]
• 5.Lameire N, Van Biesen W, Vanholder R. Acute renal failure. Lancet. 2005;365:417-430. [Crossref]
• 6.Yang J, Sun BG, Min HJ, Son YB, Kim TB, Lee J, et al. Impact of acute kidney injury on long-term adverse outcomes in obstructive uropathy: a multicenter study. Sci Rep. 2021;11(1):23639. [Crossref]
• 7.Chapman CL, Johnson BD, Parker MD, Hostler D, Pryor RR, Schlader Z. Kidney physiology and pathophysiology during heat stress and the modification by exercise, dehydration, heat acclimation, and aging. Temperature (Austin). 2020;8(2):108-159. [Crossref]
• 8.Lacey J, Corbett J, Forni L, Hooper L, Hughes F, Minto G, et al. A multidisciplinary consensus on dehydration: definitions, diagnostic methods, and clinical implications. Ann Med. 2019;51(3-4):232-251. [Crossref]
• 9.Fumihiko S, Carlos RJ, Keegan R. Climate change and nephrology. Nephrol Dial Transplant. 2023;38(1):41-48. [Crossref]
• 10.Groen In 't Woud S, Reuver N, Feitz WFJ, Quaedackers JSLT, Nijman R, Steffens M, et al. Compensatory hypertrophy in paediatric patients with unilateral ureteropelvic junction obstruction. Eur Urol Open Sci. 2021;34:10-16. [Crossref]
• 11.Claesson G, Josephson S, Robertson B. Experimental partial ureteric obstruction in newborn rats. VII. Are the long-term effects on renal morphology avoided by release of the obstruction? J Urol. 1986;136:1330-1338. [Crossref]
• 12.Bratt CG, Aurell M, Nilson S. Renal function in patients with hydronephrosis. Br J Urol. 1977;49:249-252. [Crossref]
• 13.Scalera G, Tarozzi G. Sapid solutions and food intake in repeated dehydration and rehydration periods in rats. Exp Physiol. 2001;86(4):489-498. [Crossref]
• 14.Shapiro JI, Cosby RL, Chan L. 31P nuclear magnetic resonance spectral changes in obstructed or dehydrated kidney. Kidney Int. 1989;35:830-835. [Crossref]
• 15.Lucarelli G, Mancini V, Galleggiante V, et al. Emerging urinary markers of renal injury in obstructive nephropathy. Biomed Res Int. 2014;2014:303298. [Crossref]
• 16.Josephson S, Ericson AC, Sjöquist M. Experimental obstructive hydronephrosis in newborn rats. VI. Long-term effects on glomerular filtration and distribution. J Urol. 1985;133:391-396. [Crossref]
• 17.Claesson G, Swenson L, Robertson B. Experimental obstructive hydronephrosis in newborn rats. XI. One-year follow-up study of renal function and morphology. J Urol. 1989;142:1602-1608. [Crossref]
• 18.Ozturk H, Dokucu AI, Otcu S, Gezici A, Ketani A, Yildiz FR, et al. The protective effects of captopril and nitric oxide on the solitary kidney after chronic partial ureteric obstruction. BJU Int. 2001;88:93-99. [Crossref]
• 19.Cichocki P, Filipczak K, Plachcinska A, Kusmierek J. Kidney efficiency index quantitative parameter of a dynamic renal scintigraphy. II. Usefulness in the diagnosis of obstructive nephropathy. Nucl Med Rev Cent East Eur. 2020;23(2):84-88. [Crossref]
• 20.Błaszczyk M, Cichocki P, Bieńkiewicz M, Dąbrowski J, Płachcińska A, Kuśmierek J. Assessment of the relation between pelvicalyceal dilatation in ultrasound and features of obstructive uropathy in dynamic renal scintigraphy. Nucl Med Rev Cent East Eur. 2018;21(2):96-99.
• 21.Jang SJ, Choi BS, Choi SH. Evaluation of renal function in an obstructed ureter model using 99mTc-DMSA. In Vivo. 2020;34(5):2431-2435. [Crossref]
• 22.Martínez-Klimova E, Aparicio-Trejo OE, Tapia E, Pedraza-Chaverri J. Unilateral ureteral obstruction as a model to investigate fibrosis-attenuating treatments. Biomolecules. 2019;9(4):141. [Crossref]
• 23.Chevalier RL, Forbes MS, Thornhill BA. Ureteral obstruction as a model of renal interstitial fibrosis and obstructive nephropathy. Kidney Int. 2009;75(11):1145-1152. [Crossref]