Reserpin ile indüklenen deneysel fibromiyalji modelinde timokinonun analjezik etkisi

Öz

Amaç: Fibromiyalji, yaygın ağrı ve duyusal aşırı duyarlılık ile karakterize kronik bir ağrı sendromudur ve etkili, iyi tolere edilen tedavi seçenekleri halen sınırlıdır. Bu çalışmanın amacı, sıçanlarda reserpin ile indüklenen deneysel fibromiyalji modelinde timokinonun (TQ) analjezik etkisini değerlendirmektir. Yöntem: Erkek Wistar sıçanlar rastgele üç gruba ayrıldı (her grup için n = 6): kontrol, reserpin ve reserpin + timokinon. Fibromiyalji benzeri ağrı, üç ardışık gün boyunca günde bir kez subkutan reserpin (1 mg/kg) uygulanmasıyla oluşturuldu. Son reserpin enjeksiyonundan dört gün sonra timokinon (100 mg/kg) oral yoldan uygulandı. Mekanik allodini ve termal hiperaljezi, sırasıyla von Frey filament testi ve sıcak plaka testi kullanılarak; başlangıçta ve tedaviyi takiben 30, 60, 120 ve 180. dakikalarda değerlendirildi. Davranışsal değerlendirmeler, grup dağılımından habersiz bir araştırmacı tarafından gerçekleştirildi. İstatistiksel analizlerde parametrik olmayan yöntemler kullanıldı. Bulgular: Başlangıçta mekanik ve termal nosiseptif eşikler açısından gruplar arasında anlamlı bir fark saptanmadı. Timokinon tedavisi, 60, 120 ve 180. dakikalarda hem kontrol hem de yalnızca reserpin verilen gruplara kıyasla mekanik çekilme eşiklerinde anlamlı bir artışa yol açtı (p < 0,01) ve bu durum reserpinle indüklenen mekanik allodininin belirgin biçimde azaldığını gösterdi. Buna karşılık, sıcak plaka gecikme süreleri açısından herhangi bir zaman noktasında gruplar arasında istatistiksel olarak anlamlı fark gözlenmedi; ancak ileri zaman değerlendirmelerinde timokinon uygulanan grupta sayısal olarak daha yüksek gecikme süreleri dikkati çekti. Sonuç: Timokinon, reserpin ile indüklenen fibromiyalji modelinde mekanik allodini üzerinde anlamlı bir analjezik etki göstermektedir ve fibromiyaljiye eşlik eden ağrının tedavisinde potansiyel yeni bir terapötik ajan olabileceğini düşündürmektedir. Etki mekanizmasının ve modaliteye özgü analjezik profilinin aydınlatılması için ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• Fibromiyalji
• Timokinon
• Rezerpin
• Mekanik Allodini
• Deneysel ağrı modeli

Proje Numarası

This study was supported by the Selçuk University Scientific Research Projects Coordination Office (Project No: 24401114).

The analgesic effect of thymoquinone in the reserpine-induced experimental fibromyalgia model

Öz

Aim: Fibromyalgia is a chronic pain syndrome characterized by widespread pain and sensory hypersensitivity, for which effective and well-tolerated treatments remain limited. This study aimed to evaluate the analgesic effect of thymoquinone (TQ) in a reserpine-induced experimental fibromyalgia model in rats. Methods: Male Wistar rats were randomly allocated into three groups (n = 6 per group): control, reserpine, and reserpine + thymoquinone. Fibromyalgia-like pain was induced by subcutaneous administration of reserpine (1 mg/kg) once daily for three consecutive days. Thymoquinone (100 mg/kg) was administered orally four days after the final reserpine injection. Mechanical allodynia and thermal hyperalgesia were assessed using the von Frey filament test and the hot-plate test, respectively, at baseline and at 30, 60, 120, and 180 minutes following treatment. Behavioral assessments were performed by an investigator blinded to group allocation. Non-parametric statistical analyses were applied. Results: Baseline mechanical and thermal nociceptive thresholds did not differ significantly among groups. Thymoquinone treatment resulted in a significant increase in mechanical withdrawal thresholds compared with both control and reserpine-only groups at 60, 120, and 180 minutes (p < 0.01), indicating a marked attenuation of reserpine-induced mechanical allodynia. In contrast, no statistically significant differences were observed between groups in hot-plate latency times at any time point, although numerically higher latencies were noted in the thymoquinone-treated group at later assessments. Conclusions: Thymoquinone exerts a significant analgesic effect on mechanical allodynia in a reserpine-induced fibromyalgia model, suggesting its potential as a novel therapeutic agent for fibromyalgia-associated pain. Further studies are warranted to clarify its mechanism of action and modality-specific analgesic profile.

Anahtar Kelimeler

• Fibromyalgia
• Thymoquinone
• Reserpine
• Mechanical allodynia
• Experimental pain model

Destekleyen Kurum

This study was supported by the Selçuk University Scientific Research Projects Coordination Office (Project No: 24401114).

Proje Numarası

This study was supported by the Selçuk University Scientific Research Projects Coordination Office (Project No: 24401114).

Kaynakça

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