The Importance of Cited-1 and HIF-1α Immune Activity of Granulosa Cells in IVF Treatment

Yıl 2024, Cilt: 7 Sayı: 3, 132 - 135, 30.09.2024

Umut Sari , İlhan Özdemir , Dilek Doğan Baş , Şamil Öztürk

Öz

Aim: The aim of this study was to investigate the Cited-1 and HIF-1α immune activity in granulosa cells in follicular development in patients who underwent IVF for infertility.
Materials and Methods: This study was conducted on 40 patients who were admitted to the
assisted reproductive program with the complaint of infertility at the Gazi Yaşargil Training and Research Hospital Obstetrics and Gynecology Clinic IVF center between January 2022 and November 2022 and had primary or secondary infertility while starting the Ovum-Pick-Up (OPU) procedure. The fluid containing the granulosa cells was centrifuged at 3000 rpm for 10 min. The samples were fixed and processed for routine paraffine wax tissue embedding protocol. Sections were taken from paraffin blocks and immune stained with Cited-1 and HIF-1α. The preparations were examined under the microscope.
Results: HIF-1α expression was positive in membrane of granulosa cells. The nuclei were apoptotic and pyknotic. Cited1 expression was positive in membrane of granulosa cells. the cells were pyknotic.
Conclusion: The high level of HIF-1α immunopositivity and negative Cited1 immunoreactivity in the immunohistochemical staining after the granulosa cells around the oocytes collected from female patients admitted to the IVF clinic and diagnosed with infertility showed that granulosa cell viability may be important on oocyte quality.

Anahtar Kelimeler

Granulosa cells, Oocyte, Cited-1, HIF-1α

Kaynakça

• 1.Brązert M, Kranc W, Chermuła B, et al. Human ovarian granulosa cells isolated during an ivf procedure exhibit differential expression of genes regulating cell division and mitotic spindle formation. J Clin Med. 2019; 8(12): 2026. https://doi.org/10.3390/jcm8122026.
• 2.Cecconi S, Rossi G, Oddi S, et al. Role of major endocannabinoid-binding receptors during mouse oocyte maturation. Int J Mol Sci. 2019; 20(12): E2866. https://doi.org/10.3390/ijms20122866.
• 3.Hoffmann-Dishon N, Barnett-Izhaki Z, Zalko D, et al. Endocrine-disrupting chemical concentrations in follicular fluid and follicular reproductive hormone levels. J Assist Reprod Genet. 2024 Apr 1. https://doi.org/10.1007/s10815-024-03133-6.
• 4.Zhu L, Shen S, Pan C, et al. Bovine FRAS1: mRNA expression profile, genetic variations, and significant correlations with ovarian morphological traits, mature follicle, and corpus luteum. Animals (Basel). 2024; 14(4): 597. https://doi.org/10.3390/ani14040597.
• 5.Rams-Pociecha I, Mizia PC, Piprek RP. Histological Analysis of Gonadal Ridge Development and Sex Differentiation of Gonads in Three Gecko Species. Biology (Basel). 2023; 13(1): 7. https://doi.org/10.3390/biology13010007.
• 6.Huang Y, Cheng Y, Zhang M, et al. Oxidative stress and inflammatory markers in ovarian follicular fluid of women with diminished ovarian reserve during in vitro fertilization. J Ovarian Res. 2023 ;16(1): 206. https://doi.org/10.1186/s13048-023-01293-0.
• 7.Ersahin A, Celik O, Gungor ND, et al. Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation. Reprod Biol Endocrinol. 2024; 22(1): 6. https://doi.org/10.1186/s12958-023-01176-5.
• 8.Zhang Z, Shi C, Wang Z. Therapeutic Effects and Molecular Mechanism of Chlorogenic Acid on Polycystic Ovarian Syndrome: Role of HIF-1alpha. Nutrients. 2023;15(13):2833. http://doi/10.3390/nu15132833.
• 9.Azad MB, Chen Y, Henson ES, et al.. Hypoxia induces autophagic cell death in apoptosis-competent cells through a mechanism involving BNIP3. Autophagy. 2008;4(2):195-204. http://doi.org/10.4161/auto.5278
• 10.Brown AC, Muthukrishnan SD, Guay JA, et al. Role for compartmentalization in nephron progenitor differentiation. Proc Natl Acad Sci USA. 2013;110(12):4640-5. http://doi.org/10.1073/pnas.1213971110
• 11.Durgun C, Aşir F. Effect of ellagic acid on damage caused by hepatic ischemia reperfusion in rats. Eur Rev Med Pharmacol Sci. 2022; 26: 8209-15. https://doi.org/10.26355/eurrev_202211_30352
• 12.Tukur HA, Aljumaah RS, Swelum AAA, et al. The making of a competent oocyte-a review of oocyte development and its regulation. J. Anim. Reprod. Biotechnol. 2020; 35: 2-11. https://doi.org/10.12750/JARB.35.1.2.
• 13.Eppig JJ. Intercommunication between mammalian oocytes and companion somatic cells. Bioessays. 1991; 13(11): 569-74. https://doi.org/10.1002/bies.950131105.
• 14.Arıkan FB, Sagsoz N. Effects of obesity on the serum BMP15, GDF9, and kisspeptin concentrations in women of reproductive age. J Med Biochem. 2023; 42(3): 392-400. https://doi.org/10.5937/jomb0-37329.
• 15.Kumar H, Choi DK. Hypoxia inducible factor pathway and physiological adaptation: a cell survival pathway? Medi. Inflamm. 2015: 584758. https://doi.org/10.1155/2015/584758.
• 16.Lee JW, Ko J, Ju C, et al. Hypoxia signaling in human diseases and therapeutic targets. Exp Mol Med. 2019; 51: 1-13. https://doi.org/10.1038/s12276-019-0235-1.
• 17.Tang Z, Xu R, Zhang Z, et al. HIF-1α Protects Granulosa Cells From Hypoxia-Induced Apoptosis During Follicular Development by Inducing Autophagy. Front Cell Dev Biol. 2021; 9: 631016. https://doi.org/10.3389/fcell.2021.631016.
• 18.Baddela VS, Sharma A, Michaelis M, et al. HIF1 driven transcriptional activity regulates steroidogenesis and proliferation of bovine granulosa cells. Sci Rep. 2020; 10(1): 3906. https://doi.org/10.1038/s41598-020-60935-1.
• 19.Gerstner JR, Landry CF. Expression of the transcriptional coactivator CITED1 in the adult and developing murine brain. Dev Neurosci. 2007; 29(3): 203-12. https://doi.org/10.1159/000096389.
• 20.Yahata T, Shao W, Endoh H, et al. Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein. Genes Dev. 2001; 15(19): 2598-612. https://doi.org/10.1101/gad.906301.
• 21.Hatzirodos N, Irving-Rodgers HF, Hummitzsch K, et al. Transcriptome profiling of granulosa cells of bovine ovarian follicles during growth from small to large antral sizes. BMC Genomics. 2014; 15: 24. https://doi.org/10.1186/1471-2164-15-24.
• 22.Zhou J, Yao W, Li C, et al. Administration of follicle-stimulating hormone induces autophagy via upregulation of HIF-1α in mouse granulosa cells. Cell Death Dis. 2017 Aug 17;8(8):e3001. http://doi.org/10.1038/cddis.2017.371
• 23.Kim J, Bagchi IC, Bagchi MK. Signaling by hypoxia-inducible factors is critical for ovulation in mice. Endocrinology. 20069;150(7):3392-400. https://doi.org/10.1210/en.2008-0948
• 24. Li C, Zhou J, Liu Z. FSH prevents porcine granulosa cells from hypoxia-induced apoptosis via activating mitophagy through the HIF-1α-PINK1-Parkin pathway. FASEB J. 2020;34(3):3631-45. http://doi.org/10.1096/fj.201901808RRR
• 25.Tang Z, Xu R, Zhang Z. HIF-1α Protects Granulosa Cells From Hypoxia-Induced Apoptosis During Follicular Development by Inducing Autophagy. Front Cell Dev Biol. 2021;9:631016. http://doi.org/10.3389/fcell.2021.631016