THE INFLUENCE OF RADIOTHERAPY ON CIRCULATING miRNA EXPRESSION LEVELS AND HEMORHEOLOGICAL PROPERTIES IN PROSTATE CANCER

Yıl 2017, Cilt: 3 Sayı: 1, 42 - 49, 30.03.2017

Nazlı Eyvan Topçu Özden Fatma Behice Cinemre Hakan Cinemre Selim Ögüt Nurten Bahtiyar Didem Karaçetin Haldun Şükrü Erkal Birsen Aydemir

Öz

Background: In prostate cancer, radiotherapy has therapeutic
effect and increase treatment efficacy and thus the survival rate. However
radio-resistant tumors may relapse and metastases. In cancer cells, some genes
effected by radiation has direct effects on results of radiotherapy. MikroRNAs
(miRNAs) are the molecules which regulate some processes related with internal
and external stresses. It has been showed that radiation resulted in some
changes on synthesis of miRNAs and so, cellular responses in tumor cells. The
aim of our study was to investigate expression levels of miR-20a and miR-106b
and some biochemical parameters, and plasma viscosity in patients with prostate
cancer, before and after radiotherapy.

Materials
and Methods: 35 patients
who admitted to Radiation Oncology Department for prostat cancer radiotherapy
were included in this study. Blood samples were obtained before and after
radiotherapy. miR-20a and miR-106b expressions were analyzed by using
quantitative reverse-transcription polymerase chain reaction (qRT-PZR). Plasma
viscosity values were measured by using Harkness capillary viscometer. Blood
albumin and total protein levels were analyzed by autoanalyser system.
Leukocyte, lymphocyte, erythrocyte, platelet and neutrophil counts were
measured by automatic cell counter.

Results:
We found that miR-20a
and miR-106b expression levels, and plasma viscosity values increased in the
patients after radiotherapy. We also found that erythrocyte and platelet
counts, albumin and total protein levels did not significantly change while PSA,
fPSA, leukocyte, lymphocyte and neutrophil counts significantly decreased in
patients after radiation treatment. From Pearson’s rank correlation analysis, we
found that miR-20a expression levels positively correlated with miR-106b
expression levels after radiotherapy group (r=0.722, p=0.01). The levels of
fPSA correlated significantly with miR-106b expression levels before
radiotherapy group (r=-0.598, p=0.014). Additionally, the leukocyte count
correlated significantly with miR-20a expression levels before radiotherapy
group (r=−0.474, p=0.035).  

 

 

 

 

 

Conclusion: Our findings showed that expression levels of some
miRNAs such as miR-20a and miR-106b and hemorheological parameters were changed in prostate cancer patient after
radiation treatment. These changes might be an important factor for cancer treatment
and metastasis. Effects of these changes on prostate cancer patients must be
clarified with further studies. 

Anahtar Kelimeler

Radiotherapy, prostate cancer, miR-20a, miR-106b, plasma viscosity

Kaynakça

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