Mycobacterium tuberculosis ve Mycobacterium bovis protein profillerinin karşılaştırmalı çalışması

Öz

Amaç: İlaç direncine rağmen Mycobacterium bovis ve Mycobacterium tuberculosis (MTB) hala dünyadaki küresel sağlık sorunlarından iki tanesi olarak kabul edilmektedir. Bu çalışmada, tuberküloz tanısı için etkili biyobelirteçler elde etmek amacıyla Mycobacterium bovis ve MTB protein profilleri arasında bir karşılaştırma yapılmıştır.

Yöntemler: Klinik örnekler, balgam ve gastrik lavaj (ve diğer örnekler), N-asetil-L-sistein-sodyum hidroksit yöntemleri ile işlendi ve sonuç olarak Lowenstein-Jensen ortamı üzerinde kültürlendi. Mycobacterium tuberculosis ve bovis suşları biyokimyasal testlere ve duyarlılık test sistemine göre ayrıldı. Koloniler 7H9 ortamında büyütülmüş ve membran ve sekretuar proteinler ekstrakte edilmiş, amonyum sülfat ve soğutulmuş alkol metotları ile saflaştırılmıştır.

Bulgular: Protein içerikleri Bradford metodu ile ölçüldü. Her bir suşta protein bantlarının karşılaştırması tek boyutlu bir elektroforez ile gerçekleştirilmiştir. Bant ayırma membranı proteinlerindeki iki suş arasındaki temel farklılık, 45 ve 60 KDa'da ve ayrıca 45 ve 14 KDa'dan daha az olarak gözlemlenebildi.

Sonuç: Sonuçlar, protein bantlarındaki farklılıkların tuberküloz tanısı için protein etkin biyobelirteç olarak kullanılabileceğini gösterdi. Hızlı tuberküloz teşhisi için ileri tetkiklerde tuberküloz’a karşı antikor kullanmalıyız.

Anahtar Kelimeler

• Mycobacterium bovis,Mycobacterium tuberculosis,Membran ve sekretuvar protein

Comparative study of Mycobacterium tuberculosis and Mycobacterium bovis protein profiles

Abstract

Aim: Despite the drug resistance Mycobacterium bovis and Mycobacterium tuberculosis (MTB) are still regarded as two of the global health problems in the world. In the present study, a comparison was made between protein profiles of Mycobacterium bovis and MTB in order to achieve effective biomarkers for diagnosis of tuberculosis. 

Methods: The clinical samples, sputum and gastric lavage (and the other samples) were processed by N-acetyl-L-cysteine-sodium hydroxide methods and consequently were cultured on Lowenstein–Jensen medium. Mycobacterium tuberculosis and bovis strains were distinguished according to the biochemical tests and susceptibility testing system. Colonies were grown in 7H9 medium and membrane and secretory proteins were extracted, purified by ammonium sulfate and refrigerated alcohol methods. 

Results: The protein contents were measured by Bradford method. Comparison of protein bands in each strain were performed by one dimensional electrophoresis. 

The major discrepancy between the two strains in the banding separation membrane proteins could be observed in 45 and 60 KDa and also less than 45 and 14 KDa. 

Conclusion: The results showed that discrepancy in the proteins bands could be used as protein effective biomarker for tuberculosis diagnosis. We should use antibody against tuberculosis for further investigation for rapid tuberculosis diagnosis.

Keywords

• Mycobacterium bovis,Mycobacterium tuberculosis,Membrane and Secretory protein

Kaynakça

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