Kril yağının deneysel priapizm modelinde iskemik reperfüzyon hasarı üzerine koruyucu etkileri

Yıl 2019, , 371 - 376, 28.05.2019

Engin Kölükçü , Nihat Uluocak , Velid Unsal

https://doi.org/10.28982/josam.560609

Öz

Amaç: Mevcut çalışmanın amacı ratlara uyarlanmış priapizm modelinde krill yağının priapizm kaynaklı iskemi-reperfüzyon hasarı üzerindeki etkilerinin değerlendirilmesidir.    

Yöntemler: Toplam 24 adet rat her bir grupta 8 rat bulunacak şekilde randomize edildi. Grup 1 kontrol grubu olarak belirlendi. Deneysel priapizm modeli Grup 2 ve 3'teki ratlara 1 saat süreyle uygulandı ve ardından iskemi-reperfüzyon hasarını değerlendirmek için priapizm 30 dakika süre ile sonlandırıldı. Grup 1 ve 2 deki ratlara standart yem ve musluk suyu verildi. Grup 3: Aynı beslenme prosedürü deney hayvanlarına uygulandı, ancak bir ay boyunca krill yağı ile takviye edildi. Deney sonunda süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px), malondialdehid (MDA), nitrik oksit (NO), protein karbonil (PC) düzeylerinin belirlenmesi amacı ile tüm ratlara penektomi uygulanarak vena kava inferiordan kan örneği alındı.

Bulgular: Penil dokuların biyokimyasal değerlendirmesinde Grup 3’teki MDA ve PC seviyeleri Grup 2’e göre anlamlı derecede düşük gözlemlenirken GSH-Px aktiviteleri anlamlı düzeyde yüksek olarak izlendi (sırasıyla P=0.001, P=0.005, P=0.003). Serum analiz sonuçları ise Grup 3’teki MDA ve NO seviyelerini Grup 2’e göre anlamlı derecede düşük olduğunu gösterirken SOD aktivitesini anlamlı düzeyde yüksek olduğunu ortaya koydu (sırasıyla P=0.011, P=0.001, P=0.009).

Sonuç: Bu çalışmada krill yağının; kavernosal dokuda gelişen priapizm ile indüklenen iskemi-reperfüzyon hasarına karşı koruyucu etkinliği biyokimyasal kanıtlara dayanılarak gözlenmiştir.

Anahtar Kelimeler

Krill yağı, İskemi, Reperfüzyon, Priapizm

Protective effects of krill oil on ischemic reperfusion injury in experimental model of priapism

Öz

Aim: The aim of the study is to evaluate the effects of krill oil on priapism induced ischemia-reperfusion injury in priapism rat model. 

Methods: Total of 24 rats were randomly divided into three groups with eight rats in each group. Group 1 was determined as the control group. Experimental priapism model was constructed in rats in Groups 2 and 3 for 1 hour and then priapism was terminated for 30 minutes to evaluate ischemia-reperfusion injury. The rats in Groups 1 and 2 were given tap water and standard chow pellets. Group 3: The same feeding procedure was applied to the experimental animals but supplemented with krill oil for one month. At the end of the experiment penectomy were performed, and blood samples were withdrawn from inferior vena cava of the rats to determine the levels of protein carbonyl (PC), malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in systemic circulation, and cavernosal tissues.

Results: Biochemical examination of penile tissues showed that MDA and PC levels in Group 3 were significantly lower than group 2, while GSH-Px activities were significantly increased (P=0.001, P=0.005, P=0.003 respectively). Serum analysis results showed that MDA and NO levels in Group 3 were significantly lower than Group 2 and SOD activities significantly increased (P=0.011, P=0.001, P=0.009, respectively).

Conclusion: In this study, protective effect of krill oil against priapism induced ischemia-reperfusion injury in cavernosal tissue was observed based on biochemical evidence.

Anahtar Kelimeler

Krill oil, Ischemia, Reperfusion, Priapism

Kaynakça

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