Deneysel epidural fibrosis modelinde dextpanthenol’ün epidural fibrosis oluşumu üzerine etkisi

Öz

Amaç: Spinal cerrahi sonrası gelişen epidural fibrosis dura ve spinal köklerin çevresini sıkıca sararak orta ve uzun vadede cerrahinin balşarı şansını azaltan nedenlerden biridir. Bu nedenle, bu deneysel çalışmada, ratlarda local ve sistemik dekspanthenol uygulamasının epidural fibrosis oluşumu üzerine etkisini karşılaştırmak amaç edinilmiştir.

Yöntemler: Yirmi sekiz adet sıçan rastlantısal olarak 4 eşit gruba ayrıldı (Kontrol, spongostan, local dekspanthenol ve sistemik dekspanthenol) ve bütün sıçanların torakal 11 düzeyine laminektomi yapıldı. Local olarak dexpanthenol 500 mg/kg dan spongostan ile uygulandı. Sistemik olarak dexpanthanol, 500 mg/kg dan günde bir defa olmak üzere 3 hafta boyunca uygulandı. Epidural fibrosis, araknoid tutulum, fibroblast hücre sayısı, vasküler endotelyal büyüme faktörü ve hidroksiprolini çeriği değerlendirildi.

Bulgular: Epidural fibrosis derecesi, fibroblast hücre sayısı ve hidroksiprolin düzeyi istatistiksel olarak anlamlı düzeyde sistemik dekspanthenol grubunda düşüktü (P=0,025).

Sonuç: Dekspanthenol epidural fibrosisi azaltmak için potansiyel bir ajan olabilir, fakat bu etkinin ortaya çıkması için birden fazla sayıda verilmesi gereklidir.

Anahtar Kelimeler

• Laminektomi,Epidural fibrosis,Fibroblast,Dekspanthenol,Sıçan

The effect of dexpanthenol on the formation of epidural fibrosis in an experimental laminectomy model in rats

Abstract

Aim: Epidural fibrosis, which develops after spinal surgery, is one of the factors which reduce the chances of successful surgery in the medium and long term by tightly surrounding the dura and spinal roots. In this experimental study, the aim was to compare the effects of local and systemic administration of dexpanthenol on epidural fibrosis formation in rats.

Methods: Twenty-eight rats were randomly divided into 4 equal groups (control, Spongostan, local dexpanthenol and systemic dexpanthenol) and laminectomy was performed at the T11 level. Local dexpanthenol (500mg/kg) was administered with Spongostan, and systemic dexpanthenol (500 mg/kg) was administered once a day for three weeks. Epidural fibrosis, arachnoidal involvement, fibroblast cell count, vascular endothelial growth factor, and hydroxyproline levels were evaluated. 

Results: The grade of epidural fibrosis, fibroblast cell counts, and hydroxyproline levels were significantly lower in the systemic dexpanthenol groups (P=0.025).

Conclusion: Dexpanthenol may be used as potential agent for reducing epidural fibrosis. However, it should be administered more than once for it to take effect.

Keywords

• Laminectomy,Epidural fibrosis,Fibroblast,Dexpanthenol,Rat

Kaynakça

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