Streptozotosin ile diyabet oluşturulan ratlarda alt ekstremite iskemi reperfüzyonuna karşı fullerenol C60’ın etkileri

Yıl 2020, , 451 - 455, 01.06.2020

Hakan Kartal , Ayşegül Küçük , Aydan Kılıçarslan , Yücel Polat Nuran Süngü Gülay Kip Mustafa Arslan

https://doi.org/10.28982/josam.756665

Cited By: 2

Öz

Amaç: Suda çözünür bir fulleren olan Fullerenol C60'ın, serbest radikalleri in vitro ve in vivo temizleyebildiği gösterilmiştir. Çalışmanın amacı, fullerenol C60'ın iskemi reperfüzyon (I/R) sıçan modelinde alt iskelet kasları üzerindeki etkilerini araştırmaktı.
Yöntemler: Etik kurul onayı alındıktan sonra 30 Wistar Albino sıçan; 5 gruba ayrıldı (n: 6); Kontrol (C), diyabet (grup D), diyabet + fullerenol C60 grubu (DF), diyabet + I/R (grup DIR) ve diyabet I/R + fullerenol C60 (DIR-F). Diyabet için sıçanlara 55 mg / kg streptozotosin uygulandı. Diyabet oluşumundan dört hafta sonra sıçanlara 2 saatlik iskemi ve 2 saatlik reperfüzyon uygulandı. Reperfüzyon döneminin sonunda histopatolojik ve immünohistopatolojik incelemeler için tüm gruplardan alt ekstremite iskelet kası örnekleri alındı.
Bulgular: Miyozit ve endotelyal kaspaz 3 enzim aktiviteleri, özellikle DIR ve C, D, DF ve DIR-F grubunda yüksektir. Enflamasyon DIR grubunda C, DF ve DIR-F grubuna göre anlamlı olarak yüksektir (sırasıyla P<0,001, P=0,006, P<0,001). Myosit hasarı da DIR grubunda kontrol, C, DF ve DIR-F grubuna göre anlamlı derecede yüksektir (sırasıyla P<0,001, P=0,022, P<0,001). Vasküler dilatasyon ve konjesyon D, DF, DIR ve DIR-F grubunda kontrol grubuna göre anlamlı olarak yüksektir (P<0,001, tümü).
Sonuç: Sonuçlarımız, fullerenol C60'ın diyabetik sıçanlarda I/R'den kaynaklanan iskelet kası hasarına karşı koruyucu etkileri olduğunu doğrulamaktadır. Fullerenol C60'ın I/R hasarı üzerindeki etkilerini değerlendirmek için yapılacak gelecekteki çalışmalar, fullerenol C60'ın olası koruyucu etkilerini ve I/R hasarına bağlı doku hasarının altında yatan mekanizmaları anlamaya yardımcı olabilir.

Anahtar Kelimeler

İskemi reperfüzyon, Fullerenol C60, Kaspaz 3, Sıçan

Destekleyen Kurum

YOK

The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats

Öz

Aim: Fullerenol, a water-soluble C60-fullerene, has been demonstrated to scavenge free radicals in vitro and in vivo. The aim of the study was to investigate the effects of fullerenol C60 on lower skeletal muscles in a rat model of ischemia/reperfusion (I/R).
Methods: After approval of the ethics committee, 30 Wistar Albino rat were divided into 5 groups with six animals per each as follows: Control (C), diabetes (D), diabetes+fullerenol C60 (DF), diabetes+I/R (group DIR) and diabetes I/R+fullerenol C60 (DIR-F) groups. Streptozotocin was administered to the rats to induce diabetes at a dose of 55 mg/kg. Four weeks after the onset of diabetes, rats were subjected to 2 hours of ischemia and 2 hours of reperfusion. At the end of the reperfusion period, skeletal muscle samples were taken from the lower extremity in all groups for histopathological and immunohistopathological examinations.
Results: Myositis and endothelial caspase 3 enzyme activities were high in all groups, particularly DIR. Compared to C, DF and DIR-F groups, inflammation and myositis were significantly higher in the DIR group (P=0.001, P=0.006, P=0.001, respectively, and P=0.001, P=0.022, P=0.001, respectively). Vascular dilatation and congestion were significantly more prominent in all groups compared to the control group (P=0.001 for all).
Conclusion: Our results confirm that fullerenol C60 has protective effects against skeletal muscle damage resulting from I/R in diabetic rats. Future studies conducted to evaluate these effects may help illuminate the action mechanism of fullerenol C60 and pathophysiology underlying the tissue damage related to I/R injury.

Anahtar Kelimeler

Ischemia reperfusion, Fullerenol C60, Caspase 3, Rat

Kaynakça

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