Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy

Abstract

Aim: There is very limited information concerning gestational course of chronic inflammatory diseases of the upper airway. In the current study, we tried to reveal biomolecular changes in the nasal mucosa during pregnancy regarding NFκb and MUC5AC. This way, we may have the opportunity to suggest new treatment strategies for pregnancy induced nasal disorders. Methods: Thirty adult female rats were separated into pregnant and control groups. Serum estradiole (E2) and progesterone (PG) levels were evaluated by ELISA. Nasal expression of NFκb and MUC5AC were analyzed by polymerase chain reaction testing. Relative expression of NFκb and MUC5AC was compared between two groups. In addition, the influences of PG and E2 levels on NFκb and MUC5AC were determined. Results: NFκb was significantly low (P=0.015), while MUC5AC was significantly high in pregnant rats (P=0.029). Serum PG had a significantly negative effect on NF-κB expression (P=0.027) and a significantly positive effect on MUC5AC expression (P=0.017). There was a positive correlation between estradiol and MUC5AC (P=0.017). Conclusions: In the current study, the physiological expression of NFκb and MUC5AC was shown for the first time by PCR in rat nasal mucosa. The effect of PG and E2 on the expression of these biomolecules in the context of pregnancy was also revealed. These findings have the potential to elucidate gestational inflammatory alterations in the upper airway.

Keywords

• pregnancy
• immunity
• NFκb
• MUC5AC
• nasal mucosa

Gebelik sırasında nazal mukozada NFκb ve MUC5AC ekspresyon düzeylerinin değişimi

Öz

Amaç: Üst solunum yolunun kronik enflamatuar hastalıklarının gebelikteki seyri konusunda çok sınırlı bilgi mevcuttur. Bu çalışmada, NFκb ve MUC5AC ile ilgili olarak hamilelik sırasında nazal mukozadaki biyomoleküler değişiklikleri ortaya çıkarmaya çalıştık. Bu sayede, gebeliğe bağlı burun rahatsızlıkları için yeni tedavi stratejileri önerme fırsatımız olabilir. Yöntemler: Sıçanlar üzerinde yapılan bu çalışma, Deney Hayvanları Araştırma ve Uygulama Merkezinde yapılmıştır. Otuz yetişkin Wister albino dişi sıçan, bir kontrol grubu ve bir hamile grubu olarak ayrıldı. Serum estradiole (E2) ve progesteron (PG) düzeyleri ELISA ile değerlendirildi. NFκb ve MUC5AC'nin nazal ekspresyonu, polimeraz zincir reaksiyon testi ile analiz edildi. İstatistiksel analiz, NFkb ve MUC5AC ekspresyonunun grup bazında karşılaştırılması ile yapıldı. Ayrıca E2 ve PG seviyelerinin bu moleküller üzerindeki etkisi de araştırıldı. Bulgular: Gebe sıçanlarda NFκb anlamlı olarak düşük (P=0,015) bulunurken MUC5AC anlamlı derecede yüksek bulundu (P=0.029). Serum PG ve NF-κB ekspresyonu arasında istatistiksel olarak anlamlı negatif korelasyon (P=0,027), progesteron ile MUC5AC ekspresyonu arasında ise pozitif korelasyon (P=0,017) bulundu. Ek olarak, estradiol ve MUC5AC arasında pozitif korelasyon tespit ettik (P=0.017). Sonuçlar: Bu çalışmada, sıçan nazal mukozasında NFκb ve MUC5AC'nin fizyolojik ekspresyonu ilk kez PCR ile gösterilmiştir. E’ ve PG’nin bu biyomoleküllerin ekspresyonu üzerindeki etkisi de gebelik bağlamında ortaya konmuştur. Bu bulgular, üst solunum yolunun gestasyonel enflamatuar değişikliklerini açıklama potansiyeline sahiptir.

Anahtar Kelimeler

• Gebelik
• Bağışıklık
• NFκb
• MUC5AC
• Nazal mukoza

Kaynakça

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