PRP tedavisinin endometriyum ve yumurtalıklar üzerindeki uzun vadeli ve kısa vadeli etkileri üzerine deneysel bir çalışma

Yıl 2020, Cilt: 4 Sayı: 12, 1195 - 1200, 01.12.2020

Özcan BUDAK Veysel TOPRAK Can TÜRKLER

https://doi.org/10.28982/josam.824815

Öz

Amaç: Yumurtalıkların kök hücrelere sahip olduğu ve yumurtalık rezervi düşük olan hastaların bazı büyüme faktörleri ile olgunlaştırılarak tedavisinde kullanılabileceği bildirilmiştir. Yeni bir tedavi olan PRP, büyüme faktörleri dahil olmak üzere birçok biyoaktif bileşiğe sahiptir. Bu çalışma, sıçanlarda trombositten zengin plazma (PRP) tedavilerinin uterus ve yumurtalıkların iç yüzeyine uzun ve kısa vadeli biyokimyasal ve histopatolojik etkilerini araştırmayı amaçladı.
Yöntemler: Dişi sıçanlar (n=21) rastgele 3 gruba ayrıldı: Sham grup, kısa süreli bir PRP grubu ve bir uzun süreli PRP grubu. Çalışma sonunda intrakardiyak alandan alınan kan örneklerinde anti-Mullerian hormonu (AMH) ve folikül stimüle edici hormon (FSH) seviyeleri incelendi ve ratların yumurtalık ve uterusu incelendiğinde histopatolojik ve immünohistokimyasal bulgular.
Bulgular: Sıçanların kan örneklerinde kısa süreli PRP grubunda AMH düzeylerinin arttığını ve FSH düzeylerinin düştüğünü göstermiştir (P<0.05). Sıçanların rahim ve yumurtalıkları histopatolojik olarak incelendiğinde, kısa süreli PRP grubunda PRP tedavisinin olumlu ve faydalı etkileri tespit edildi. Ancak uzun vadeli sonuçlar dikkate alındığında PRP'nin olumlu etkileri zaman geçtikçe azalmaktadır.
Sonuç: PRP uygulamasının kısa vadede dişi sıçanlarda rahim ve yumurtalıklara faydalı etkiler sağladığını göstermiştir.

Anahtar Kelimeler

Anti-Mullerian hormone, Follicle-stimulating hormone, Over, Platelet-Rich plasma, Rat, Endometriyum

An experimental study on the long-term and short-term effects of PRP treatment on the endometrium and ovaries

Öz

Aim: It has been reported that ovaries have stem cells, and they can be used in the treatment of patients with low ovarian reserves by maturing them through some growth factors. PRP, a new therapy, has many bioactive compounds including growth factors. This study aimed to investigate the long and short-term biochemical and histopathological effects of platelet-rich plasma (PRP) treatments on the lining of the uterus and the ovaries in rats.
Methods: Female rats (n = 21) were randomly divided into 3 groups: A sham group, a short-term PRP group and a long-term PRP group. At the end of the study, anti-Mullerian hormone (AMH) and follicle-stimulating hormone (FSH) levels were examined in blood samples obtained from the intracardiac area, and the ovaries and uterus of the rats were examined to determine the histopathological and immunohistochemical findings.
Results: The results of this study demonstrated that, in the blood samples of the rats, the AMH levels increased and FSH levels decreased in the short-term PRP group (P<0.05). When the uteri and ovaries of the rats were examined histopathologically, positive and beneficial effects of the PRP therapy were found in the short-term PRP group. However, considering the long-term results, the positive effects of PRP decrease as time goes on.
Conclusion: This study showed that PRP application delivers beneficial effects to the uterus and ovaries in female rats in the short term.

Anahtar Kelimeler

Anti Mullerian Hormone, follicle stimulating hormone, ovary, platalet-rich plasma, rat, endometrium

Kaynakça

• 1. Garcia-Velasco JA, Acevedo B, Alvarez C, Monica A , Jose B , Juan F, et al. Strategies to Manage Refractory Endometrium: State of the Art in 2016. Reprod Biomed Online. 2016;32(5):474-89.
• 2. Sills ES, Wood SH. Autologous Activated Platelet-Rich Plasma Injection Into Adult Human Ovary Tissue: Molecular Mechanism, Analysis, and Discussion of Reproductive Response. Biosci Rep. 2019;39(6):BSR20190805.
• 3. Lubkowska A, Dolegowska B, Banfi G. Growth Factor Content in PRP and Their Applicability in Medicine. J Biol Regul Homeost Agents. 2012;26(2 Suppl 1):3S-22S.
• 4. Kaya F, Kismet K, Ozer H, Soylu VG, Duymus ME, Akgun YA et al. Can Platelet-Rich Plasma Be Used Safely in Intra-Abdominal Operations? Bratisl Lek Listy. 2016;117(9):525-29.
• 5. Knezevic NN, Candido KD, Desai R, Kaye AD. Is Platelet-Rich Plasma a Future Therapy in Pain Management? Med Clin North Am. 2016;100(1):199-217.
• 6. Magalon J, Bausset O, Serratrice N, Laurent G, Houssein A, Julie V et al. Characterization and Comparison of 5 Platelet-Rich Plasma Preparations in a Single-Donor Model. Arthroscopy. 2014;30(5):629-38.
• 7. Bakacak M, Bostanci MS, İnanc F, Yaylali A, Serin S, Attar R, et al. Protective Effect of Platelet Rich Plasma on Experimental Ischemia/Reperfusion Injury in Rat Ovary. Gynecol Obstet Invest. 2016;81(3):225-31.
• 8. Hosseini L, Shirazi A, Naderi MM, Shams-Esfandabadi N, Borjian Boroujeni S, Sarvari A et al. Platelet-rich Plasma Promotes the Development of Isolated Human Primordial and Primary Follicles to the Preantral Stage. Reprod Biomed Online. 2017;35(4):343-50.
• 9. Sfakianoudis K, Simopoulou M, Nitsos N, Rapani A, Pantou A, Vaxevanoglou T et al. A Case Series on Platelet-Rich Plasma Revolutionary Management of Poor Responder Patients. Gynecol Obstet Invest. 2019;84(1):99-106.
• 10. Sfakianoudis K, Simopoulou M, Nitsos N, Rapani A, Pappas A, Pantou A, et al. Autologous Platelet-Rich Plasma Treatment Enables Pregnancy for a Woman in Premature Menopause. J Clin Med. 2018;8(1):1.
• 11. Nagata MJ, Messora MR, Furlaneto FA, Fucinia S. E. , Boscoa A. F, Garciaa V G, et al. Effectiveness of Two Methods for Preparation of Autologous Platelet-Rich Plasma: An Experimental Study in Rabbits. Eur J Dent. 2010;4(4):395-402.
• 12. Jang HY, Myoung SM, Choe JM, KimT, Cheon YP, KimYMet al. Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats. Yonsei Med J. 2017;58(6):1195-203.
• 13. Zhang S, Li P, Yuan Z, Tan J. Platelet-rich Plasma Improves Therapeutic Effects of Menstrual Blood-Derived Stromal Cells in Rat Model of Intrauterine Adhesion. Stem Cell Res Ther. 2019;10(1):61.
• 14. Irkorucu O, Ferahköşe Z, Memiş L, Ekinci O, Akin M. Reduction of Postsurgical Adhesions in a Rat Model: A Comparative Study. Clinics (Sao Paulo). 2009;64(2):143-8.
• 15. Yokoyama Y, Takahashi Y, Morishita S, Hashimoto M, Niwa K, Tamaya T. Telomerase Activity in the Human Endometrium Throughout the Menstrual Cycle. Mol Hum Reprod. 1998;4(2):173-7.
• 16. McCampbell AS, Walker CL, Broaddus RR, Cook JD, Davies PJ. Developmental Reprogramming of IGF Signaling and Susceptibility to Endometrial Hyperplasia in the Rat. Lab Invest. 2008;88(6):615-26.
• 17. Li X, Sun H, Lin N, Hou X, Wang J, Zhou B, et al. Regeneration of Uterine Horns in Rats by Collagen Scaffolds Loaded With Collagen-Binding Human Basic Fibroblast Growth Factor. Biomaterials. 2011;32(32):8172-81.
• 18. Ribeiro AA, Martin I, Derussi AA, Ferioli RB, Laufer-Amorim R, Lopes MD. Histological Characterization and Immunohistochemical Localization of ki-67 and VEGF Factors in Bitches' Corpus Luteum at Cyclic and Early and Late Gestational Diestrous. Reprod Domest Anim. 2012;47 Suppl 6:61-5.
• 19. Nazari L, Salehpour S, Hoseini S, Zadehmodarres S, Ajori L. Effects of autologous platelet-rich plasma on implantation and pregnancy in repeated implantation failure: A pilot study. Int J Reprod Biomed (Yazd). 2016;14(10):625-8.
• 20. Sipahi M. Effects of Autologous Platelet-Rich Plasma on Endometrium Thickness and Pregnancy Rates During Intrauterine Insemination. Middle Black Sea Journal of Health Science. 2019; 5(2):63-6.
• 21. Apte RS, Chen DS, Ferrara N. VEGF in Signaling and Disease: Beyond Discovery and Development. Cell. 2019;176(6):1248-64.
• 22. Sun X, Kaufman PD. Ki-67: more than a proliferation marker. Chromosoma. 2018;127(2):175-186.
• 23. Chang Y, Li J, Chen Y, Wei L, Yang X, Shi Y, et al. Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization. Int J Clin Exp Med. 2015;8(1):1286-90.
• 24. Sfakianoudis K, Simopoulou M, Nitsos N, Lazaros L, Rapani A, Pantou A et al. Successful Implantation and Live Birth Following Autologous Platelet-rich Plasma Treatment for a Patient with Recurrent Implantation Failure and Chronic Endometritis. In Vivo. 2019;33(2):515-21.
• 25. Wu CK, Wang YC, Lee JK, Chang SN, Su MY, Yeh HM, et al. Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan diastolic heart failure registry and molecular basis by cellular and animal models. Eur J Heart Fail. 2014;16(2):163-72.
• 26. Spartalis E, Damaskos C, Athanasiou A. Safety of platelet-rich plasma application in abdominal operations. Bratisl Lek Listy. 2017;118(7):437.