Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida
Abstract
Many chemicals used in industry are produced using different isomers of muconic acid. The isomers are especially precursors for many industrially important polymers. In this study, eco-friendly production of cis, cis-muconic acid (ccMA) used in the production of polymers was carried out. Statistical experimental design was applied to optimize the production of ccMA by Pseudomonas putida KT2440 in batch culture. Plackett-Burman design was used to investigate the effect of 11 variables for ccMA production in the first optimization step. The results showed that ccMA production was most influenced by the initial pH of the medium and concentrations of Na-benzoate, NaCl, and (NH4)2SO4. After these significant factors were determined, the optimization of these factors was carried out with Response Surface Methodology (RSM) as the second optimization step. RSM showed that the optimal values of the factors (the initial pH and concentrations of Na-benzoate, NaCl, and (NH4)2SO4) were 7.96, 16.83 mM, 0.7 and 1.4 g/L, respectively. The other components of the fermentation medium were 18 g/L Na2HPO4, 10 g/L KH2PO4, 20 mM glucose, 1 mM MgSO4, 150 µM CaCl2, 13 µM FeSO4, and temperature of 35 oC. ccMA production increased about 4 times using statistical optimization methods.
Keywords
cis-cis muconic acid Pseudomonas putida fermentation Plackett-Burman response surface methodology
Ethical Statement
There is not ethical statement
Supporting Institution
The Scientific Research Projects of Ankara University (BAP)
Project Number
17L0443004
Thanks
The authors thank to The Scientific Research Projects of Ankara University
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Details
| Primary Language | English |
|---|---|
| Subjects | Chemical Engineering (Other) |
| Journal Section | Research Article |
| Authors | |
| Project Number | 17L0443004 |
| Submission Date | February 2, 2026 |
| Acceptance Date | February 23, 2026 |
| Publication Date | March 21, 2026 |
| DOI | https://doi.org/10.58692/jotcsb.1880143 |
| IZ | https://izlik.org/JA86RT72WR |
| Published in Issue | Year 2026 Volume: 2026 |
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