THE EFFECT OF OZONE MEDIATED PRECONDITIONING ON FLAP VİABİLİTY IN RATS: AN EXPERİMENTAL STUDY

Öz

Objectives: There are many type of preconditioning techniques in flap surgery. But ozone mediated preconditioning method was not studied sufficiently. The main purpose of our study is to show the effect of preoperative ozone gas on flap viability and to reveal that ozone therapy can be used as a preconditioning method in skin flaps. Methods: 40 rats were randomly divided into 4 groups and a caudal-based 11x3 cm modified Mac Farlane flap was elevated for all subjects. At the end of one week, back flaps were removed and evaluated macroscopically and histopathologically. Results: In terms of flap viability, all groups were statistically better than the control group not only macroscopically but also hystopathologically. Chronic inflammation scores, amount of granulation tissue and neovascularization were higher in all groups and especially in the preoperative ozone group compared to the control group (p <0.001). Conclusion: In our study, it has been shown that ozone mediated pretreatment method has both macroscopically increased flap survival and beneficial effects at histopathological level.

Anahtar Kelimeler

• Skin flap
• ozone gas
• ozone mediated preconditioning
• rectal ozone therapy.

OZON ARACILI ÖN KOŞULLANDIRMANIN SIÇANLARDA FLEP YAŞAYABILIRLIĞI ÜZERINE ETKISI: DENEYSEL BIR ÇALIŞMA

Öz

Giriş: Flep cerrahisinde birçok ön koşullandırma tekniği vardır. Ancak ozon aracılı ön koşullandırma yöntemi yeterince çalışılmamıştır. Çalışmamızın temel amacı, ameliyat öncesi ozon gazının flep canlılığı üzerindeki etkisini göstermek ve cilt fleplerinde ozon tedavisinin bir ön koşullandırma yöntemi olarak kullanılabileceğini ortaya koymaktır. Metot: 40 adet sıçan rastgele 4 gruba ayrıldı ve tüm deneklere kaudal tabanlı 11x3 cm modifiye Mac Farlane flebi kaldırıldı. Bir hafta sonunda sırt flepleri çıkarılarak makroskopik ve histopatolojik olarak değerlendirildi. Bulgular: Flep canlılığı açısından, tüm gruplar sadece makroskopik olarak değil aynı zamanda histopatolojik olarak da kontrol grubuna göre istatistiksel olarak daha iyiydi. Kronik inflamasyon skorları, granülasyon dokusu miktarı ve neovaskülarizasyon tüm gruplarda ve özellikle preoperatif ozon grubunda kontrol grubuna göre daha yüksekti (p<0,001). Sonuç: Çalışmamızda ozon aracılı ön tedavi yönteminin hem makroskopik olarak flep sağkalımını arttırdığı hem de histopatolojik düzeyde faydalı etkileri olduğu gösterilmiştir.

Anahtar Kelimeler

• Cilt flep
• ozon gazı
• ozon aracılı ön koşullandırma
• rektal ozon tedavisi.

Kaynakça

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