EFFECTS OF GINGER EXTRACTS ON TOTAL PROTEIN AMOUNT AND PEROXIDASE ACTIVITY IN SOLANUM LYCOPERSICUM L.

Abstract

Zingiber officinale Roscoe, belonging to the Zingiberaceae family, is a medicinal plant that also spreads in our country. In this research, extracts of Z. officinale rhizomes were prepared with different solvents (ethanol, methanol, distilled water, DMSO). Zingiber rhizome extracts were sprayed on the leaves of rio grande and marmande varieties of 10-12 weeks old Solanum lycopersicum plantlets. Total protein amount and peroxidase activity were examined by spectrophotometrically 24 and 48 hours after the application. According to the results, it was determined that the maximum increase in the total protein amount compared with the control group was 31.31% and 25.53% in marmande and rio grande varieties in ginger rhizome extract prepared with methanol, respectively. The increase in peroxidase activity was determined as 144.14% and 124.85% in marmande and rio grande varieties in ginger rhizome extract prepared with ethanol, respectively. As a result of our research, it was determined that rhizome extracts of Z. officinale can be used as natural plant activator.

Keywords

• Zingiber officinale,Solanum lycopersicum,total protein,peroxidase

Kaynakça

1. ACIBUCA, V., BUDAK, D. B., 2018. Dünya’da ve Türkiye’de Tıbbi ve Aromatik Bitkilerin Yeri ve Önemi. Çukurova Tarım ve Gıda Bilimleri Dergisi, 33(1), 37-44.
2. BRADFORD, M. M., 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantites of Protein Utilizing The Principle of Protein Dye Binding. Analytical Biochemistry, 72: 248-254.
3. DİNÇ, A.G., and AKI, C., 2015. Effects of Echinacea angustifolia Hell. extracts on total protein and peroxidase changing in Solanum lycopersicum L., Annals of Biological Research, 6 (12), 25-29.
4. DÜNDAR, Y., 2001. Fitokimyasallar ve Sağlıklı Yaşam, Afyon Kocatepe Üniversitesi, Kocatepe Tıp Dergisi, 2,131-138
5. FAYDALIOĞLU, E., SÜRÜCÜOĞLU, M. S., 2011. Geçmişten Günümüze Tıbbi ve Aromatik Bitkilerin Kullanılması ve Ekonomik Önemi. Kastamonu Üniversitesi, Orman Fakültesi Dergisi 11 (1):52-67.
6. FELTER, H.W., LOYD, J.U., 2002. Zingiber (U.S.P.) Ginger. King's American Dispensatory. www.ibiblio.org/hermed/eclectic/kinga/zingiber.
7. GANN, P.H., MA, J., CIOVANNUCCI, E., WILLETT, W., SACKS, F.M., HENNEKENS, C.H., STAMPFER, M.J., 1999. Lower prostate cancer risk in man with elevated plasma lycopene levels: results of a prospective analysis. Cancer Research, 59:(6), 1225-1230.
8. JANA, U., CHATTOPADHYAY, N.R., SHAW, B., 1999. Preliminary Studies On Anti- Inflammatory Activityy of Zingiber officinale Rosc., Vitex negundo Linn. and Tinospora cordifolia (willid) miers in Albino Rats., Indian Journal of Pharmacology 31, 232-233.

9. KABELKA, E., YANG, W., FRANCIS, D.M., 2004. Improved tomato fruit color within an inbred backcross line derived from Lycopersicon esculentum and L. hirsutum involves the interaction of loci. Journal of the American Society for Horticultural Science, 129 (2):250-257.
10. KANNER, J., KINSELLA, J.E., 1983. Lipid Deterioration Initiated by Phagocytic Cells in Muscle Foods: β-Carotene Destruction by a Myeloperoxidase-Hydrogen Peroxide-Halide System. Journal of Agricultural and Food Chemistry, 31: 370-376.
11. KAYA, Y., AL-REMİ, F., ARVAS, Y.E., DURMUŞ, M., 2018. Domates Bi̇tki̇si̇ Ve in Vi̇tro Mi̇kro Çoğaltımı (Tomato Plant and Its In Vitro Micropropagation). Journal of Engineering Technology and Applied Sciences, 3(1), 57-73.
12. KOÇ, E., ÜSTÜN, A.S., 2008. Patojenlere karşı bitkilerde savunma ve antioksidanlar. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi 24 (1), 82-100.
13. LIU, Y, WHELAN, RJ, PATTNAIK, BR, LUDWIG, K, SUBUDHI, E, ROWLAND, H, CLAUSSEN, N, ZUCKER, N, UPPAL, S, KUSHNER, DM, FELDER, M, PATANKAR MS, KAPUR A., 2012. Terpenoids from Zingiber officinale (Ginger) induce apoptosis in endometrial cancer cells through the activation of p53. PLoS ONE, 7, (12).
14. RAAOF, A., AL-NAQQASH, Z.A., JAWAD, A.M, MUHSAN, S.M., 2013. Evaluation of the Activity of Crude Alkaloids Extracts of Zingiber officinale Roscoe., Thymus vulgaris L. and Acacia arabica L. as Coagulant Agent in Lab Mice, Biomedicine and Biotechnology, 1 (2), 11-16.
15. SÖNMEZ, K., ELLİALTIOĞLU, Ş., 2014. Domates, karotenoidler ve bunları etkileyen faktörler üzerine bir inceleme, Derim, 31 (2): 107-130.
16. TÜRKÜSAY, H., TOSUN, N., 2005. Hidrojen Peroksit Uygulamalarının Domates Bakteriyel Solgunluk ve Kanser Hastalığı (Clavibacter michiganensis spp. michiganensis (Smith) Davis et al)’na Etkileri. Ege Üniversitesi Ziraat Fakültesi Dergisi, 42(2), 45-56.
17. YILDIZ, C., AKI, C., 2018. Prunus spinosa L. ve Rubus sanctus Schreb. Yaprak Ekstraktlarının Capsıcum annuum L. Çeşitlerinde Toplam Protein Miktarı ve Peroksidaz Aktivitesi Üzerine Etkilerinin Belirlenmesi, Journal of Awareness, 3(5), 609-616.
18. YILDIZ, C., AKI, C., 2019. Effects of Blackberry and Blackthorn Fruit Extracts on Protein and Peroxidase Activity In Pepper. Journal of Scientific Perspectives, 3(3), 237-244.