In this study, the antimicrobial activity, DNA cleavage, DNA binding and
antioxidant properties of a sulfonic acid-based imine compound were investigated.
The antimicrobial activity of the compound was investigated for minimum
inhibitory concentration (MIC) against
some bacteria and yeast cultures.
The DNA cleavage activity of the compound was investigated as hydrolytic
and oxidative with the gel electrophoresis method. H2O2 was used as an
oxidizing agent for detection of the cleavage activity mechanism. The
Ultraviolet-Visible (UV-Vis) field absorption spectroscopy method was used to
determine the binding effect to DNA. The sulfonic acid-based imine compound
reacted with Calf Thymus DNA (CT-DNA) which was examined by UV-Vis absorption
spectroscopy. The free radical scavenging activity was determined using the
2,2-Diphenyl-1-picrylhydrazyl (DPPH) method.
The studied compound was found to be effective on yeast and bacteria at
different concentrations. The compound was found to be more effective on
Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 bacteria.
DNA cleavage study showed that the compound cleaved DNA without any external
agents in hydrolytic and oxidative manner. UV-Vis spectroscopy studies of the
interactions between the compound and CT-DNA showed that the compound interacts
with CT-DNA via electrostatic binding. The compound to be tested was compared
with the butylated hydroxytoluene (BHT) solution used as standard. It was found
that the compound exhibits good antioxidant activity.
Sulfonic acid antimicrobial activity antioxidant activity DNA cleavage DNA binding.
In this study, the antimicrobial activity, DNA cleavage, DNA binding and
antioxidant properties of a sulfonic acid-based imine compound were investigated.
The antimicrobial activity of the compound was investigated for minimum
inhibitory concentration (MIC) against
some bacteria and yeast cultures.
The DNA cleavage activity of the compound was investigated as hydrolytic
and oxidative with the gel electrophoresis method. H2O2 was used as an
oxidizing agent for detection of the cleavage activity mechanism. The
Ultraviolet-Visible (UV-Vis) field absorption spectroscopy method was used to
determine the binding effect to DNA. The sulfonic acid-based imine compound
reacted with Calf Thymus DNA (CT-DNA) which was examined by UV-Vis absorption
spectroscopy. The free radical scavenging activity was determined using the
2,2-Diphenyl-1-picrylhydrazyl (DPPH) method.
The studied compound was found to be effective on yeast and bacteria at
different concentrations. The compound was found to be more effective on
Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 bacteria.
DNA cleavage study showed that the compound cleaved DNA without any external
agents in hydrolytic and oxidative manner. UV-Vis spectroscopy studies of the
interactions between the compound and CT-DNA showed that the compound interacts
with CT-DNA via electrostatic binding. The compound to be tested was compared
with the butylated hydroxytoluene (BHT) solution used as standard. It was found
that the compound exhibits good antioxidant activity.
Sulfonic acid antimicrobial activity antioxidant activity DNA cleavage DNA binding.
Birincil Dil | İngilizce |
---|---|
Konular | Sağlık Kurumları Yönetimi |
Bölüm | Natural Sciences |
Yazarlar | |
Yayımlanma Tarihi | 29 Ekim 2019 |
Yayımlandığı Sayı | Yıl 2019 Cilt: 3 Sayı: 4 |