3-(2-Carboxyethyl)phenylboronic Acid ve 4-Aminocarbonylphenylboronic Acid Bileşiklerinin Parental ve Bortezomib-Dirençli Prostat Kanseri Hücre Hatlarındaki Etkileri

Öz

Kemoterapi kanser tedavisinde yaygın olarak kullanılan bir metod olmakla birlikte hücrelerin zamanla kemoterapötik ilaçlara karşı direnç oluşturması (kemoresistans) önemli bir sorun oluşturmaktadır. Bortezomib çeşitli kanser türlerinin tedavisinde kullanılan bor elementi içeren bir bileşiktir. Ancak pek çok çalışmada bortezomib muamelesine cevap olarak hücrelerde hızlı bir şekilde direnç oluştuğu gösterilmiştir. Bu çalışmada PC3 insan prostat kanseri hücre hattı (PC3-Parental) ve daha önceki çalışmalarda bortezomib direnci kazandırılmış PC3 hücre hatları (PC3-Dirençli) kullanılmıştır. Çalışma kapsamında PC3-Parental ve PC3-Dirençli hücreler üzerinde diğer bazı bor içeren bileşiklerin sitotoksik etkileri araştırılmıştır. 48 saat süreyle 3-(2-Carboxyethyl)phenylboronic acid ile muamele sonrasında IC₅₀ değeri, PC3-Parental hücrelerde 702.7 µM; PC3-Dirençli hücrelerde ise 1156 µM olarak tespit edilmiştir. 4-Aminocarbonylphenylboronic acid bileşiğinin 48 saat muamele sonrasında IC₅₀ değeri, PC3-Parental hücrelerde 117.3 µM; PC3-Dirençli hücrelerde ise 226.3 µM olarak bulunmuştur. Özellikle 4-Aminocarbonylphenylboronic acid bileşiği daha düşük bir IC₅₀ değerine sahip olduğu için, daha detaylı analizler ile etkilerinin araştırılması faydalı olacaktır. Ayrıca, farklı ilaç kombinasyonu denemeleri ile daha düşük konsantrasyonlarda daha etkili bir kanser tedavi yaklaşımı geliştirilebileceği düşünülmektedir.

Anahtar Kelimeler

• 3-(2-Carboxyethyl)phenylboronic acid
• 4-Aminocarbonylphenylboronic acid
• bortezomib
• prostat kanseri

The Effects of 3-(2-Carboxyethyl)phenylboronic Acid and 4-Aminocarbonylphenylboronic Acid Compounds on Parental and Bortezomib-Resistant Prostate Cancer Cell Lines

Öz

Although chemotherapy is a widely used method in cancer treatment, the development of resistance to chemotherapeutic agents over time (chemoresistance) constitutes a major problem. Bortezomib is a boron element-containing compound used in the treatment of various cancer types. However, many studies have demonstrated that cells rapidly develop resistance in response to bortezomib treatment. In this study, the PC3 human prostate cancer cell line (PC3-Parental) and previously established bortezomib-resistant PC3 cell lines (PC3-Resistant) were used. Within the scope of the study, the cytotoxic effects of several other boron-containing compounds were investigated on PC3-Parental and PC3-Resistant cells. After 48 hours of treatment with 3-(2-Carboxyethyl)phenylboronic acid, the IC₅₀ value was determined as 702.7 µM in PC3-Parental cells and 1156 µM in PC3-Resistant cells. Following 48 hours of treatment with 4-Aminocarbonylphenylboronic acid, the IC₅₀ values were found to be 117.3 µM in PC3-Parental cells and 226.3 µM in PC3-Resistant cells. Since 4-Aminocarbonylphenylboronic acid exhibited a lower IC₅₀ value, further detailed analyses would be beneficial to investigate its effects more comprehensively. Additionally, it is thought that a more effective cancer treatment strategy at lower concentrations could be developed through trials of different drug combinations.

Anahtar Kelimeler

• 3-(2-Carboxyethyl)phenylboronic acid
• 4-Aminocarbonylphenylboronic acid
• bortezomib
• prostate cancer

Kaynakça

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