Determination of in Vitro Effects of Metal Ions (Zn+2, Co+2, Cd+2, Ni+2) on the Hepatopancreas Esterase (E. C. 3.1.1.1) Activity of Callinectes sapidus (Blue Crab)

Öz

In this study, in vitro effects of zinc (Zn), cobalt (Co), cadmium (Cd), nickel (Ni) on the hepatopancreas esterase (E. C. 3.1.1.1) activity of Callinectes Sapidus (Rathbun, 1896), a crab species consumed as a food by humans, were investigated. According to the obtained results; IC50 values were found to vary between 0.83 mM (Cd) and 32.21 mM (Co), in addition to the inhibition effect of all the metal ions investigated on the hepatopancreas esterase activity. When assessed the inhibition types of the metal ions investigated, while Zn and Co caused un-competitive inhibition, Cd and Ni had exhibited mixed type inhibition. The data obtained provide biochemical data on the role of blue crab in determination of environmental metal pollution.

Anahtar Kelimeler

• Callinectes sapidus
• Hepatopancreas
• Esterase
• Metal ions

Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi

Öz

Bu çalışmada insanlar tarafından besin olarak tüketilen bir yengeç türü olan Callinectes sapidus (Rathbun, 1896)’nın hepatopankreas esteraz (E. C. 3.1.1.1) aktivitesine çinko (Zn), kobalt (Co), kadmiyum (Cd), nikel (Ni) metallerinin etkileri in vitro olarak belirlenmiştir. Elde edilen sonuçlara göre; araştırılan tüm metal iyonlarının hepatopankreas esteraz aktivitesi üzerine inhibisyon etkisi sergilemekle birlikte, IC50 değerlerinin 0.83 mM (Cd) ile 32.21 mM (Co) arasında değişim gösterdiği bulunmuştur. Metal iyonlarının inhibisyon tipleri değerlendirildiğinde, Zn ve Co un-kompetitif inhibisyona neden olurken, Cd ve Ni karışık tip inhibisyon sergilemişlerdir. Elde edilen veriler, çevresel metal kirliliğinin belirlenmesinde mavi yengecin rolüne biyokimyasal veriler sunmaktadır.

Anahtar Kelimeler

• Callinectes sapidus
• hepatopankreas
• esteraz
• metal iyonları

Kaynakça

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