Piperazinin Galleria mellonella’nın Yaşama ve Gelişimi Üzerine Etkisi

Yıl 2018, Cilt: 8 Sayı: 1, 365 - 372, 01.01.2018

Nur Emine Sefer

Öz

Piperazin hayvanların paraziter enfeksiyonlarında antihelmintik bir ilaç olarak kullanılır. Heksahidropirazin grubu bir antihelmintik olan piperazin heksahidratın farklı besinsel konsantrasyonlarının Büyük bal mumu güvesi Galleria mellonella L. Lepidoptera: Pyralidae larvalarının ergin evreye kadar yaşama oranına ve gelişme süresine etkisi incelendi. Birinci evre larvaları farklı konsantrasyonlarda piperazin % 0,001, 0,01, 0,1 ve 1 içeren yapay besinlerde erginleşinceye kadar yetiştirildi. Piperazin heksahidratın tüm besinsel konsantrasyonları larval evrede 7. evre ve larva sonrası evrelerde pup ve ergin evreler yaşama oranını önemli derecede düşürdü. Kontrol besininde larvaların % 98,75±1,08’i 7. evreye ulaşırken bu larvaların % 90,00±2,5’i pup evresine, % 83,75±3,24’ü ise ergin evreye ulaştı. Piperazinin en yüksek konsantrasyonunu içeren besin 7. evreye ulaşan larvaların oranını % 26,25±5,96’ya, pup olma oranını % 21,25±5,69’a, ergin olma oranını ise % 20,00±6,37’ye önemli derecede düşürdü. Bu antihelmintik maddenin düşük konsantrasyonlarını içeren besinler ile beslenen larvaların 7. larval evreye ulaşma süreleri ve pup olma sürelerinin istatistiksel olarak etkilenmediği belirlendi. Buna karşılık ergin evreye ulaşma süresi piperazin heksahidratın %0,1’lik konsantrasyonundan itibaren önemli derecede uzadı. Besine ilave edilen en yüksek piperazin konsantrasyonu % 1 böceğin birinci larval evreden ergin evreye kadar tüm gelişimini önemli derecede geciktirdi. Piperazinin bu konsantrasyonu ergin olma süresini 37,66±0,77 günden 41,32±2,31 güne ortalama 4 gün önemli derecede uzatmıştır. Piperazin insan ve diğer hedef olmayan organizmalara karşı düşük akut toksisiteye sahip olduğundan, konsantrasyonlarının iyi ayarlanması ile insektisit olarak kullanılabilir

Anahtar Kelimeler

Galleria mellonella, Piperazin, Yaşama oranı, Beslenme

The Effect of Piperazine on Survivorship and Development of Galleria mellonella

Öz

Piperazine is used in animal parasitic enfection as an anthelmintic drug. The effects of different dietary concentrations of piperazine, which is belonging to hexahydropirazine compound, on survivorship and developmental time throughout adult emergence of Greater wax moth, Galleria mellonella L. Lepidoptera: Pyralidae larvae were investigated. First instar larvae were reared on diets containing different concentrations of piperazine 0.001, 0.01, 0.1 ve 1 % to adult emergence. All dietary concentration of piperazine significantly decreased survivorship on larval 7th-instar and postlarval stages pupa and adults . Control diet produced 7th instars of 98.75±1.08 %, pupae of 90.00 %±2.5, and adults of 83,75±3,24 %. The diet with the highest concentration of piperazine hexahydrate significantly decreased the survivorship of 7th instars to 26,25±5,96 %, pupation to 21,25±5,69 % and adult emergence to 20,00±6,37 %. It was recorded that the diets with low concentrations of this antihelmintic had no significant effects on developmental time to 7th instar and pupation. However, developmental time to adult emergence was significantly prolonged by 0.1 % of piperazine. The highest concentration of piperazine 1 % resulted in significant increase in whole developmental time from first instar to adult emergence. This concentration of piperazine significantly prolonged adult developmental time from 37.66±0.77 to 41.32±2.31 days by approximately 4 days. Because of relatively low acute toxicity of piperazine to human and other animals as nontarget organisms

Anahtar Kelimeler

Galleria mellonella, piperazine, survivorship, nutrition

Kaynakça

• Arbo, M.D., Bastos M.L., Carmo, H.F. 2012. Piperazine compounds as drugs of abuse. Drug Alcohol Depend. 22(3):174- 85.
• Austin, H., Monasterio, E., 2004. Acute psychosis following ingestion of ‘Rapture’. Australas Psychiatry. 12: 406-408.
• Bolleddula, J., DeMent, K., Driscoll, J.P., Worboys, P., Brassil P.J., Bourdet. D.L. 2014. Biotransformation and bioactivation reactions of alicyclic amines in drug molecules. Drug Metab. Rev. 46 (3): 379-419.
• Bowden, K., Ross, W.J. 1965. A comparison of anthelmintic and antibacterial activity of some phloroglucinol derivatives. J. Pharm. Pharmacol. 17(4): 239–242.
• Bronskill, J. 1961. A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pralidae). J. Lep. Soc. 15 (2: 102- 104.
• Bueding, E., Saz, H.J., Farrow, G.W. 1959. The effect of pıperazine on succinate productıon by Ascaris lumbricoides. Brit. J. Pharmacol. 14: 497-500.
• Büyükgüzel, E., Kalender, Y. 2007. Penicillin-induced oxidative stress: effects on antioxidative response of midgut tissues in larval instars of G. mellonella. J. Econ. Entomol. 100: 1533- 1541.
• Büyükgüzel, E., Kalender, Y. 2008. Galleria mellonella survivorship, development and protein content in response to dietary antibiotics. J. Entomol. Sci. 43: 27-40.
• Büyükgüzel, E., Kalender, Y. 2009. Exposure to streptomycin alters oxidative and antioxidative response in larval midgut tissues of Galleria mellonella. Pest. Biochem. Physiol. 94: 112- 118.
• Büyükgüzel, E., Kayaoğlu, S. 2014. The effect of niclosamide on some biological and physiological aspects of Galleria mellonella L. (Lepidoptera: Pyralidae). Türk Entomol Derg. 38 (1): 83-99.
• Büyükgüzel, K. 2001a. Positive Effects of Some Gyrase Inhibitors on Survival and Development of Pimpla turionellae (Hymenoptera: Ichneumonidae) Larvae Reared on an Artificial Diet. J. Econ. Entomol. 94 (1): 21-26.
• Büyükgüzel, K. 2001b. DNA Gyrase Inhibitors: Novobiocin enhances the survival of Pimpla turionellae larvae reared on an artificial diet but other antibiotics do not. J. Appl. Entomol. 125: 583-587.
• Büyükgüzel, K., Yazgan, Ş. 1996. Bazı Antibiyotiklerin Endoparazitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae)’nın yaşama ve Gelişimine Etkileri. Turk. J. Zool. 20: 1-7.
• Büyükgüzel, K., Yazgan Ş. 1999. Combinational Effects of Some Antimicrobial Agents on the Survival and Development of the Endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae). Communications (séries C.). 48:1-14.
• Campos, F., Donskov, N., Arnason, J.T., Philogene, B.J.R., Atkinson, P.M., Werstiuk, N.H. 1990. Biological effects and toxicokinetics of DIMBOA in Diadegma terebrans (Hymenoptera: Ichneumonidae), an endoparasitoid of Ostrina nubilalis (Lepidoptera: Pyralide). J. Econ. Entomol. 83: 356- 360.
• Charriere, J.D., Imdorf, A. 1997. Protection of honeycombs from moth damage. Swiss Bee Research Center Federal Dairy Research Station. Comm. 24: 1-14.
• Clark, A.G., Dick, G.L., Martindale, S.M., Smith, J.N. 1985. Glutathione s-transferases from the New Zealand grass grub. Costelytra zealandica. Insect Biochem. 15: 35-44.
• Constanti, A., Nistri A. 1976. A comparatıve study of the actıon of g-aminobutyric acid and piperazine on the lobster muscle fibre and the frog spinal cord. Br. J. Pharmac. 57: 347-358.
• Costa, H.S., Thomas, J.H., Nick, C.T. 1997. Effect of antibacterial materials on Bemisia argentifolii (Homoptera: Aleyrodidae) oviposition, growth, survival and sex ratio. J. Econ. Entomol. 90: 333-339.
• Çalık, G., Büyükgüzel, K., Büyükgüzel, E. 2016. Reduced fitness in adults from larval, Galleria mellonella (Lepidoptera: Pyralidae) reared on media amended with the antihelmintic, mebendazole. J. Econ. Entomol. 109 (1): 182-187.
• Del Castillo, J., Demello, W.C., Morales, B.T. 1963. Mechanism of the paralysing actıon of piperazine on ascaris muscle. Brit. J. Pharmacol. 22: 463-477.
• Doss, G.A., Miller, R.R., Zhang, Z., Teffera, Y., Nargund, R.P., Palucki, B., Park, M.K., Yui S. Tang, Evans, D.C., Baillie, T.A., Stearns, R.A. 2005. Metabolic activation of a 1,3-Disubstituted piperazine derivative: Evidence for a novel ring contraction to an imidazoline. Chem. Res. Toxicol. 18: 271- 276.
• Fiakpui, E. Z. 1967. Some Effects of piperazine and methyridine on the free-living nematode Caenorhabditis Briggsae (Rhabditidae). Nematologica, 13 (2): 241-255
• Grenier, S. 1977. Effects nocif de la nipagine M sur le parasitoide Phryxe caudata [Dipt.: Tachinidae]. Entomophaga, 22(2): 223- 236.
• Grenier, S., Liu, W.H. 1990. Antifungals: Mold control and safe levels in artificial media for Trichogramma [Hymenoptera: Trichogrammatidae]. Entomophaga. 35(2): 283-291.
• Grenier, S., Liu, W.H. 1991. Mold control and safe levels of antifungals in artificial media for egg parasitoids (Hymenoptera). Les Colloques de l’INRA. 56 : 141-144.
• Hamzaoğlu, M. 2012. Diritromisinin Galleria mellonella (Lepidoptera: Pyralidae)’nın bazı biyolojik ve biyokimyasal özelliklerine etkisi. Bülent Ecevit Üniversitesi, Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, Zonguldak.
• Haroz, R., Greenberg. M. 2006. New drugs of abuse in North America. Clin. Lab. Med. 26: 147-164.
• Hız, P., Erdem, M., Büyükgüzel, E., Büyükgüzel, K. 2016. Gemifloksasinin Galleria mellonella L. (Lepidoptera: Pyralidae) erginlerinin bazı biyolojik özelliklerine etkisi, Kafkas Univ. Vet. Fak. Derg. 22 (5): 777-784.
• Hondebrink L., Hermans E.J, Schmeink S, van Kleef R.G, Meulenbelt J, Westerink R.H. 2015. Structure-dependent inhibition of the human α1β2γ2 GABAA receptor by piperazine derivatives: A novel mode of action. Neurotoxicology. 51: 1-9.
• Kastamonuluoğlu, S. 2013. Terbinafinin Galleria mellonella L (Lepidoptera: Pyralidae)’nın bazı biyolojik ve biyokimyasal parametrelerine etkisi. Bülent Ecevit Üniversitesi, Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, Zonguldak.
• Kılıç, A., Büyükgüzel, K., Büyükgüzel, E. 2015. Antihelmintik triklabendazolun yapay besin ile beslenen Galleria Mellonella (Lepidoptera: Pyralidae) larvalarının yaşama ve gelişimine etkisi.Kafkas Univ. Vet. Fak. Derg. ( J. Fac. Vet. Med. Kafkas Univ.). 21(6): 841- 847 (DOI: 10.9775/kvfd.2015.13731).
• Kulkarni, N, Kushwaha, D.K, Mishara, V.K, Paunikar, S. 2012. Effect of economical modification in artificial diet of Greater wax moth Galleria mellonella (Lepidoptera: Pyralidae), Indian J. Entomol. 74 (4): 369-374.
• Lingala, S., Nerella, R., Rao, K.R.S.S. 2011. Synthesis, antimicrobial and anthelmintic activity of some novel benzimidazole derivatives. Der Pharma Chemica. 3 (4): 344-352.
• Martin, R.J. 1982. Electrophysiological effects of piperazine and diethylcarbamazine on Ascaris suum somatic muscle. Br. J. Pharmacol. 77: 255–265.
• Martin, R.J. 1985. Gamma-Aminobutyric acid-and piperazineactivated single-channel currents from Ascaris suum body muscle. Br. J. Pharmacol. 84: 445–461.
• Martin, R.J. 1997. Modes of action of anthelmintic drugs. Vet. J. 154: 11–34.
• Maurer, H.H., Thomas, K., Dietmar S., Roland F.S. 2004. Chemistry, pharmacology, toxicology, and hepatic metabolism of designer drugs of the amphetamine (Ecstasy), piperazine, and pyrrolidinophenone types: A synopsis. Ther Drug Monit. 26: 127-131.
• Ndhlala, A.R., Ghebrehiwot, H.M., Ncube,B., Aremu, A.O., Gruz, J., Šubrtová, M., Doležal, K., du Plooy, C.P., Abdelgadir, H.A., Van Staden, J. 2015. Antimicrobial, anthelmintic activities and characterisation of functional phenolic acids of Achyranthes aspera Linn.: A medicinal plant used for the treatment of wounds and ringworm in East Africa. Front Pharmacol. 6: 274.
• Nielsen, S.A., Toft, S. 2002. Responses of a detoxification ezyme to diet quality in the wolf spider. Pardosa prativaga. European Arachnology, eds. Toft S. and Scharff N., Aarhus University Pres, Aarhus, pp. 65-70.
• Ortel, J. 1995. Accumulation of cd and pb in successive stages of Galleria mellonella and metal transfer to the pupal parasitoid Pimpla turionellae. Entomol Exp. Appl. 77: 89-97.
• Parvatham, K., Veerakumari, L. 2013. Drug target prediction using elementary mode analysis in Ascaris lumbricoides energy metabolism. Biotechnol. Bioprocess Eng. 18: 491-500.
• Ramarao,N.,Nielsen-Leroux, C., Lereclus, D. 2012. The Insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. J. Vis. Exp. 70: 4392.
• Singh, P., House, H.L. 1970a. Antimicrobials safe levels in a synthetic diet of an insect, Agria affinis. J. Insect Physiol. 16: 1769-1782.
• Singh, P., House, H.L. 1970b. Effects of streptomycin and potassium sorbate in relation to nutrient levels on the larvae of Agria affinis. J. Econ. Entomol. 63: 449-454.
• Singh, P., House, H.L. 1970c. Antimicrobial agents: their detrimental effects on size of an insect, Agria affinis. Can. Entomol. 102: 1340-1344.
• Slansky, JrF., Scriber, J.M. 1985. Food consumption and utilization. Comprehensive Insect Physiology, Biochemistry and Pharmacology, eds. Kerkut G. A. and Gilbert L. I. Pergamon Pres, Oxford, pp. 87-163.
• Snedecor, G.S., Cochran, W.G. 1989. Statistical Methods, Iowa State University Press, 8th ed., Ames, IA. 158-160.
• SPSS. 1997. User’s manual, version 10. SPSS, Chicago, IL.
• Sugeçti, S., Büyükgüzel, E., Büyükgüzel, K. 2016. Laboratory Assays of the Effects of Oxfendazole on Biological Parameters of Galleria mellonella (Lepidoptera: Pyralidae). J.Entomol. Sci. 51 (2): 129-137
• Vuran, E. 2012. Ornidazolun Galleria mellonella (Lepidoptera: Pyralidae) ’nın bazı biyolojik ve biyokimyasal parametrelerine etkisi. Bülent Ecevit Üniversitesi, Fen Bilimleri Enstitüüs Yüksek Lisans Tezi, Zonguldak.
• Xie, Z.N, Nettles, Jr C.W, Morrison R.K, Irie, K, Vinson, S.B. 1986. Three methods for the In vitro culture of Trichogramma pretiosum Riley. J. Entomol. Sci. 21(2): 133-138.
• Yazgan, Ş. 1972. A chemically-defined synthetic diet and larval nutritional requirements of the endoparasitoid Itoplectis conquisitor (Hymenoptera). J. Insect Physiol 18: 2123-2141.
• Yazgan, Ş. 1981. A meridic diet and quantitative effects of tween 80, fatty acid mixtures and inorganic salts on development and survival of the endoparasitoid Pimpla turionellae. L Z angew Ent. 91: 433-441.