Karbon Nanotüp Katkılı Biyosensör Tasarımı ve Biyosensörün Çevresel Kirletici Olan Fenol ve Türevlerinin Tespitinde Kullanılması

Yıl 2016, Cilt: 6 Sayı: 2, 399 - 405, 01.06.2016

Muhammet Samet Kılıç Şeyda Korkut

Öz

Bu çalışmada, su kaynakları ve atıksularda izlenmesi gereken önemli kirletici parametrelerden olan fenol ve fenol türevlerinin güvenli, seçici, hassas ve hızlı ölçülmesi amacıyla karbon nanotüp ile modifiye edilmiş iletken polimerik film kaplı çalışma elektrodu tabanlı fenol biyosensörü geliştirilmiştir. Fenol oksidasyonundan sorumlu olan tyrosinase enzimi çalışma elektrodu üzerine immobilize edilmiştir. Tasarlanan biyosensörde 18 farklı fenol türü analiz edilmiş, ölçüm için analitik parametreler hesaplanarak sonuçlar tartışılmıştır. Biyosensörün test edilen 5 fenol türüne tepki göstermesi, tyrosinase enziminin farklı fenolikler için geliştirilecek biyosensörlerde yüksek seçicilikte bir enzim olduğunu göstermiştir. Sistemde, karbon nanotüpün elektroaktiviteyi artırarak biyosensör sinyal gücüne katkıda bulunduğu sonucuna varılmıştır

Anahtar Kelimeler

Amperometrik sensör, Fenol biyosensörü, İletken polimerler, Tyrosinase

Design of Carbon Nanotube Embedded Biosensor and Its Usage for Detection of Phenol and Its Derivatives Which are Environmental Pollutants

Öz

In this study, a phenol biosensor based on the carbon nanotube modified conductive polymeric film coated working electrode has been developed for safe, selective, sensitive and fast detection of phenol and its derivatives, which are important pollutants should be detected in water sources and wastewaters. Enzyme tyrosinase responsible for phenol oxidation has been immobilized onto the working electrode. Various 18 phenol derivatives have been analyzed by the designed biosensor, and the results have been discussed by calculating the analytical parameters of the measurement. Response of the biosensor to 5 phenolics among the tested phenols showed that the tyrosinase was highly selective enzyme for biosensors would be developed for different phenols. It was concluded that carbon nanotube contributed to the biosensor signal by enhancing the electroactivity.

Anahtar Kelimeler

Amperometric sensor, Phenol biosensor, Conductive polymers, Tyrosinase

Kaynakça

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