Asiklovirin Galleria mellonella L. Larvalarının Yetiştirilmesinde Kullanılan Yapay ve Doğal Besinlerdeki Mikrobiyal Kontaminasyona Etkisi

Yıl 2016, Cilt: 6 Sayı: 1, 105 - 110, 01.01.2016

Ender Buyukguzel Kemal Buyukguzel

Öz

Klinik öneme sahip antiviral asiklovirin bal arısı kovanlarının önemli bir zararlısı olan büyük bal mumu güvesi Galleria mellonella L.’nın larvalarını laboratuvar şartlarında yetiştirmek için kullanılan yapay Bronskill besini ve doğal besinlerdeki kabartılmış boş koyu renkli eski petek; kuluçka peteği mikrobiyal kontaminasyon kaynaklarına etkisi incelendi. Larvalar asiklovirin farklı konsantrasyonlarını % 0.001-3.0 içeren besinler ile yetiştirildi. Kontrol grubunda larvalar asiklovir içermeyen besin ile yetiştirildi. Asiklovirin % 1.0’lık konsantrasyonunu içeren besinin böceğin ergin öncesi ve ergin evredeki biyolojik parametreleri üzerinde önemli bir olumsuz etkiye sahip olmadığı belirlendi. Bu sebepten dolayı böceğe karşı olumsuz etkiye sahip olmayan bu konsantrasyonun besinlerdeki antimikrobiyal etkisi incelendi. Antimikrobiyal etki beslenme ortamına, mikrobial kaynakların başlangıçtaki yoğunluklarına ve mikrobiyal organizmaların türüne göre değişti. Asiklovirin % 1.0’lık konsantrasyonu yapay besindeki maya ve küf sayısını sırasıyla 6000 ± 565.6 ve 800 ± 20.6 CFU’den 800 ± 35.3 and 50 ± 2.3 CFU’e önemli derecede düşürürken, Stahylococcus aureus 3200 ± 141.4 CFU bakterisinin çoğalmasını tamamen önledi. Yapay besinde koliform bakteriler bulunmazken doğal besinde 1100 ± 84.8 CFU olarak tespit edilen koliform bakterilerin çoğalması tamamen önlendi. Asiklovirin % 1.0’lık konsantrasyonunun besin ortamlarındaki bakteri, küf ve maya kontaminasyonunu önlemede etkili olduğu belirlendi. Bu çalışma bir antimikrobiyal maddenin böceklerin laboratuvar şartlarında yetiştirilmesi sırasında katkı maddesi olarak besine ilave edilmeden önce antimikrobiyal etkisinin belirlenmesi gerektiğinin önemini belirtmektedir

Anahtar Kelimeler

Asiklovir, Antimikrobiyal etki, Yapay ve doğal besinler, Galleria mellonella

Effect of Acyclovir on the Microbial Contamination in the Artifical and Natural Diets for Rearing of Galleria mellonella L. larvae

Öz

The effects of clinically important antiviral agent, acyclovir, on microbial contamination sources in the artificial Bronskill diet and natural diets dark old honeycomb used for rearing of a serious pest in the honey bee hives, greater wax moth Galleria mellonella L. The larvae were reared on diets conatining different concentrations of acyclovir 0.001-3.0 % . Control larvae were reared on diet without acyclovir. The diet with 1.0% of acyclovir had no significant negative effects on biological parameters of immature stages and adult stage. Therefore, dietary antimicrobial effects of this safe concentration on insect were tested. Antimicrobial effects varied with the rearing media, initial microbial sources in diets and microorganism types. Acyclovir at 1.0 g in artificial diet was significantly capable of reducing the number of yeast and molds from 6000 ± 565.6 and 800 ± 20.6 CFU to 800 ± 35.3 and 50 ± 2.3 CFU respectively. Proliferation of Stahylococcus aureus 3200 ± 141.4 CFU inhabiting this diet were completely inhibited by 1.0 g of acyclovir. Coliform bacteria are not seen in the artificial diet. This concentration of acyclovir completely inhibited proliferation of coliform in natural diet while the artificial diet lacks coliform bacteria. Acyclovir at a level of 1.0% was also of capable of preventing the bacterium, mold and yeast growth in both artificial diet and natural food. We infer from these results that the importance of determining the antimicrobial effect of an antimicrobial agent before adding it as a food additive to the diet during laboratory rearing of insects.

Anahtar Kelimeler

Galleria mellonella, acyclovir, artificial and natural diets, antimicrobial effect

Kaynakça

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