Kahramanmaraş Topraklarından İzole Edilen Bacillus sp. Tarafından Alfa-Amilaz Üretimi ve Karakterizasyonu

Yıl 2015, Cilt: 5 Sayı: 2, 68 - 74, 01.06.2015

Ashabil Aygan

Öz

Bacillus sp. A-3 Kahramanmaraş topraklarından izole edilmiştir. Suş 5.0-7.0 pH aralığında enzim sentezini gerçekleştirirken, maksimum amilolitik aktivite 37oC sıcaklık ve 6.5 pH değerinde gözlenmiştir. SDS-PAGE analizinde enzime ait 53.94 kDa ağırlığında tek bant elde edilmiştir. Enzimin optimum pH’sı 6.0-6.5 olarak belirlenmiştir. Enzimin maksimum rölatif aktivite gösterdiği sıcaklık değeri 35oC olup ve 25 ile 65oC arasında ortalama %68 bir rölatif aktiviteye sahiptir. Enzim 40oC ye kadar termostabil bir özellik sergilemiştir. Kimyasalların etkisi açısından ise en yüksek kalan aktivite %93 ile KCl ve en düşük ise %0.8 ile EDTA’da gözlenmiştir. Bu özellikleri ile A-3 alfa amilazı unlu mamul sanayiinde, biyoetanol üretiminde ve hurda kâğıt endüstrisinde önemli bir yer teşkil edebilecek potansiyeldedir

Anahtar Kelimeler

Alfa amilaz, Bacillus, Karakterizasyon, Mezofilik, Saflaştırma, Stabilite

Production and Characterization of Alfa-Amylase by Bacillus sp. Isolated from Kahramanmaraş Soils

Öz

Bacillus sp. A-3 isolated from soils of Kahramanmaras. The maximum amylolitic activity was obtained at 37o C and pH 6.5 as the strain showed enzyme synthesis in between pH 5.0-7.0. SDS-PAGE analysis of the enzyme showed a single band which was estimated as 53.94 kDa. Optimum pH of the enzyme was determined as 6.0-6.5. Maximum relative activity of the enzyme presented was 35o C and also average 68% of relative activity was observed in between 25-65o C. Enzyme revealed a thermostable properties up to 40o C. In terms of chemicals effect, The highest remaining activity was 93% with KCl as the lowest one was 0.8% in the presence of EDTA. According to these properties of the A-3 alpha amylase has potential to have an important place in baking, bioethanol production and waste paper industries

Anahtar Kelimeler

Bacillus, alfa amilaz, karakterizasyon, mesofilik, saflaştırma, stabilite

Kaynakça

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